Research Data Leeds Repository

Impact on microbubble physical and mechanical properties – dataset

Abou-Saleh, Radwa H. and McLaughlan, James R. and Bushby, Richard J. and Johnson, Benjamin R. and Freear, Steven and Evans, Stephen D. and Thomson, Neil H. (2019) Impact on microbubble physical and mechanical properties – dataset. University of Leeds. [Dataset]

Dataset description

The production and stability of microbubbles (MBs) is enhanced by increasing the viscosity of the surrounding solution. Glycerol is a good candidate for biomedical applications of MBs, since it is biocompatible, although the exact molecular mechanisms of its action is not fully understood. Here we investigate the influence glycerol has on lipid-shelled MB properties, using a range of techniques. Population lifetime and single bubble stability were studied using optical microscopy.  Bubble stiffness measured by AFM compression is compared with lipid monolayer behavior in a Langmuir-Blodgett trough. We deduce that increasing glycerol concentrations enhances stability of MB populations through a three-fold mechanism. Firstly, binding of glycerol to lipid headgroups in the interfacial monolayer up to 10% glycerol, increases MB stiffness but has limited impact on shell resistance to gas permeation and corresponding MB lifetime. Secondly, increased solution viscosity above 10% glycerol slows down the kinetics of gas transfer, markedly increasing MB stability. Thirdly, above 10%, glycerol induces water structuring around the lipid monolayer, forming a glassy layer which also increases MB stiffness and resistance to gas loss. At 30% glycerol, the glassy layer is ablated, lowering the MB stiffness but MB stability is further augmented. Although the molecular interactions of glycerol with the lipid monolayer modulate the MB lipid shell properties, MB lifetime continually increases from 0 to 30% glycerol, indicating that its viscosity is the dominant effect on MB solution stability. This three-fold action and biocompatibility makes glycerol ideal for therapeutic MB formation and gives new insight into the action of glycerol on lipid monolayers at the gas-liquid interface.

Keywords: Microbubbles, Glycerol, Lipid membrane, AFM, Stability, Ultrasound
Subjects: F000 - Physical sciences
F000 - Physical sciences > F200 - Materials science
F000 - Physical sciences > F300 - Physics > F310 - Applied physics
F000 - Physical sciences > F300 - Physics
F000 - Physical sciences > F300 - Physics > F320 - Chemical physics
J000 - Technologies > J700 - Biotechnology
Divisions: Faculty of Mathematics and Physical Sciences > School of Physics and Astronomy
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License: Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited: 02 Apr 2019 12:03




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