This is the repository for data related to the paper entitled “Molecular effect of glycerol on lipid monolayers at the gas-liquid interface: Impact on microbubble physical and mechanical properties” The repository consists of all raw data for all the presented results and figures; Figure 1 folder Excel spreadsheets with raw data for microbubble sizing, and another excel spreadsheet with all raw data and analysis used for Fig. 1, Panel b. Figure 2 folder All raw data for microbubble lifetime analysis. Figure 3 folder Excel sheet for the MB dissolution data presented in Figure 3. Figure 4 folder Excel sheet with raw data for each point presented in Figure 4. Figure 5 folder Excel file, with sheets for all panels presented in Figure 5. Figure 6 folder Excel file with all Langmuir trough raw data. Figure 7 folder Excel sheet with all data points extracted from Figure 6 and used to plot Figure 7.