Research Data Leeds Repository

Dataset for 'A highly active mineral-based ice nucleating agent supports in situ cell cryopreservation in a high throughput format'

Daily, Martin and Whale, Thomas F. and Kilbride, Peter and Lamb, Stephen and Picton, Helen (2023) Dataset for 'A highly active mineral-based ice nucleating agent supports in situ cell cryopreservation in a high throughput format'. University of Leeds. [Dataset] https://doi.org/10.5518/1288

Dataset description

Cryopreservation of biological matter in microlitre scale volumes of liquid would be useful for a range of applications. At present, it is challenging because small volumes of water tend to supercool, and deep supercooling is known to lead to poor post thaw cell viability. Here we show that a mineral ice nucleator can almost eliminate supercooling in 100 microlitre liquid volumes during cryopreservation. This strategy of eliminating supercooling greatly enhances cell viability relative to cryopreservation protocols with uncontrolled ice nucleation. Using infrared thermography, we demonstrate a direct relationship between extent of supercooling and post-thaw cell viability. Using a mineral nucleator delivery system we open the door to the routine cryopreservation of mammalian cells in multiwell plates for applications such as high throughput toxicology testing of pharmaceutical products and regenerative medicine.

Keywords:	cell cryopreservation ice-nucleation ice nucleation supercooling heterogeneous ice-nucleation ice nucleation toxicology screening multiwell plates
Divisions:	Faculty of Environment > School of Earth and Environment
Related resources:	Location Type https://doi.org/10.1098/rsif.2022.0682 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	08 Feb 2023 16:10
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/1069
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Daily, Martin https://orcid.org/0000-0001-8387-1394 University of Leeds https://ror.org/024mrxd33 ror Whale, Thomas F. https://orcid.org/0000-0002-1062-2685 University of Warwick https://ror.org/01a77tt86 ror Kilbride, Peter Cytiva Lamb, Stephen Cytiva Picton, Helen University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: NERC, Leverhulme Trust, Cytiva Grant numbers: NE/L002574/1, ECF-2018-127 Publication date: 2023 Resource language: English Research project title: PhD Studentship, Early Career Fellowship Publisher: University of Leeds Contact Email Address: m.i.daily@leeds.ac.uk Last Modified: 08 Feb 2023 23:35

Files

Documentation

README_Daily_2023 [5kB] README_Daily_2023 [5kB] README_Daily_2023 [5kB] Visible to: Anyone File name: README_Daily_2023.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 5kB

Data

fig4a_water.xlsx [47kB] fig4a_water.xlsx [47kB] fig4a_water.xlsx [47kB] Visible to: Anyone File name: fig4a_water.xlsx Content type: Data Description: Droplet thermal data during 96-well plate freezing run - pure water Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 47kB

fig4bfghi_LDH1.xlsx [78kB] fig4bfghi_LDH1.xlsx [78kB] fig4bfghi_LDH1.xlsx [78kB] Visible to: Anyone File name: fig4bfghi_LDH1.xlsx Content type: Data Description: Droplet thermal data during 96-well plate freezing run - LDH1 Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 78kB

fig4c_cholesterol.xlsx [35kB] fig4c_cholesterol.xlsx [35kB] fig4c_cholesterol.xlsx [35kB] Visible to: Anyone File name: fig4c_cholesterol.xlsx Content type: Data Description: Droplet thermal data during 96-well plate freezing run - Cholesterol monohydrate Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 35kB

fig4d_snomax.xlsx [35kB] fig4d_snomax.xlsx [35kB] fig4d_snomax.xlsx [35kB] Visible to: Anyone File name: fig4d_snomax.xlsx Content type: Data Description: Droplet thermal data during 96-well plate freezing run - Snomax Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 35kB

fig4e_k-felspar.xlsx [33kB] fig4e_k-felspar.xlsx [33kB] fig4e_k-felspar.xlsx [33kB] Visible to: Anyone File name: fig4e_k-felspar.xlsx Content type: Data Description: Droplet thermal data during 96-well plate freezing run - K-feldspar bcs-376 Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 33kB

fig5_nmandfreezingtemperature.xlsx [41kB] fig5_nmandfreezingtemperature.xlsx [41kB] fig5_nmandfreezingtemperature.xlsx [41kB] Visible to: Anyone File name: fig5_nmandfreezingtemperature.xlsx Content type: Data Description: Droplet freezing temperature and Nm(T) data for LDH1 and other ice-nucleants Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 41kB

fig6abc_tnuc-survival.xlsx [17kB] fig6abc_tnuc-survival.xlsx [17kB] fig6abc_tnuc-survival.xlsx [17kB] Visible to: Anyone File name: fig6abc_tnuc-survival.xlsx Content type: Data Description: Well specific data for post-thaw viable cell number, survival compared to non-frozen baseline and ice nucleation temperature for the correlation experiment HepG2 96-well plate culture Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 17kB

fig6d_welltemptimeseries.xlsx [42kB] fig6d_welltemptimeseries.xlsx [42kB] fig6d_welltemptimeseries.xlsx [42kB] Visible to: Anyone File name: fig6d_welltemptimeseries.xlsx Content type: Data Description: Thermal histories of each well (containing cyroprotectant aliquot and HepG2 cell monolayer) of the correlation experiment culture plate Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 42kB

fig7_rawcelldata_immediate.xlsx [24kB] fig7_rawcelldata_immediate.xlsx [24kB] fig7_rawcelldata_immediate.xlsx [24kB] Visible to: Anyone File name: fig7_rawcelldata_immediate.xlsx Content type: Data Description: Post-thaw viable cell assay results for the immediate post-thaw plate freezing trial Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 24kB

fig7_rawcelldata5day.xlsx [24kB] fig7_rawcelldata5day.xlsx [24kB] fig7_rawcelldata5day.xlsx [24kB] Visible to: Anyone File name: fig7_rawcelldata5day.xlsx Content type: Data Description: Post-thaw viable cell assay results for the 5-day post-thaw plate freezing trial Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 24kB

fig7a_anovaoutputs_immediatepostthaw.docx [304kB] fig7a_anovaoutputs_immediatepostthaw.docx [304kB] fig7a_anovaoutputs_immediatepostthaw.docx [304kB] Visible to: Anyone File name: fig7a_anovaoutputs_immediatepostthaw.docx Content type: Data Description: Minitab outputs for the ANOVA significance testing for the immediate post-thaw trial cell viabilty data Mime-Type: application/vnd.openxmlformats-officedocument.wordprocessingml.document License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 304kB

fig7b_averagesurvivalanova_immediatepost_thaw.docx [84kB] fig7b_averagesurvivalanova_immediatepost_thaw.docx [84kB] fig7b_averagesurvivalanova_immediatepost_thaw.docx [84kB] Visible to: Anyone File name: fig7b_averagesurvivalanova_immediatepost_thaw.docx Content type: Data Description: Minitab outputs for the ANOVA significance testing for the overall immediate post-thaw survival Mime-Type: application/vnd.openxmlformats-officedocument.wordprocessingml.document License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 84kB

fig7d_t-testoutputs_5daypostthaw.docx [39kB] fig7d_t-testoutputs_5daypostthaw.docx [39kB] fig7d_t-testoutputs_5daypostthaw.docx [39kB] Visible to: Anyone File name: fig7d_t-testoutputs_5daypostthaw.docx Content type: Data Description: Minitab outputs for the t-test significance testing for the 5-day post-thaw trial cell viabilty data Mime-Type: application/vnd.openxmlformats-officedocument.wordprocessingml.document License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 39kB

fig7e_averagesurvivalt-test_5-daypostthaw.docx [21kB] fig7e_averagesurvivalt-test_5-daypostthaw.docx [21kB] fig7e_averagesurvivalt-test_5-daypostthaw.docx [21kB] Visible to: Anyone File name: fig7e_averagesurvivalt-test_5-daypostthaw.docx Content type: Data Description: Minitab outputs for the ANOVA significance testing for the overall 5-day post-thaw survival Mime-Type: application/vnd.openxmlformats-officedocument.wordprocessingml.document License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 21kB