Title: Dataset for Dataset for 'A HIGHLY ACTIVE MINERAL-BASED ICE NUCLEATING AGENT SUPPORTS IN-SITU CELL CRYOPRESERVATION IN A HIGH THROUGHPUT FORMAT' Creator(s): Martin I. Daily [1], Thomas F. Whale [2], Peter Kilbride [3], Stephen Lamb [3], G. John Morris [3], Helen M. Picton [1], Benjamin J. Murray [1] Organisation(s): 1. University of Leeds. 2. University of Warwick. 3 Cytiva, Cambridge, UK. Rights-holder(s):Unless otherwise stated, Copyright 2023 University of Leeds Publication Year: 2023 Description: This dataset contains experimental data underlying the study ("A highly active mineral-based ice nucleating agent supports in-situ cell cryopreservation in a high throughput format" by Daily et al (2023) published in Journal of the Royal Society Interface) including droplet freezing temperature data for LDH1 and other ice-nucleants and HepG2 cell culture post-thaw cryopreservation results. Cite as: Daily et al, (2023): Dataset for "A highly active mineral-based ice nucleating agent supports in-situ cell cryopreservation in a high throughput format", University of Leeds, https://doi.org/10.5518/1288 Contact: [m.i.daily@leeds.ac.uk] 2. TERMS OF USE --------------- Copyright 2023, University of Leeds. Unless otherwise stated, this dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/. 3. PROJECT AND FUNDING INFORMATION ---------------------------------- 1: PhD Studentship 2017-2022, NERC/UKRI, NE/L002574/1 2: Early Career Fellowship, Leverhulme Trust, ECF-2018-127 3: Cryoprotect, European Research Council, 713664 4: MarineIce, European Research Council, 648661 5: Cytiva, private funding 4. CONTENTS ----------- File listing fig4a_water.xlsx: contains thermal histories of pure water droplets in 96-well plates during a cooling run, plotted in Fig. 4a. fig4bfghi_LDH1.xlsx: contains thermal histories of pure water droplets with suspended LDH1 powder in 96-well plates during a cooling run, at concentrations of 0.01, 0.1, 1% and 10% weight/weight plotted in Fig. 4b,f,g,h,i. fig4c_cholesterol.xlsx: contains thermal histories of pure water droplets with suspended cholesterol in 96-well plates during a cooling run, at a concentration of 1% w/w, plotted in Fig. 4c. fig4d_snomax.xlsx: contains thermal histories of pure water droplets with suspended snomax in 96-well plates during a cooling run, at a concentration of 1% w/w, plotted in Fig. 4d. fig4e_k-felspar.xlsx: contains thermal histories of pure water droplets with suspended K-feldspar (BCS-376 microcline) in 96-well plates during a cooling run, at a concentration of 1% w/w, plotted in Fig. 4e. fig5_nmandfreezingtemperature.xlsx: contains droplet freezing temperatures and calculations of number of active sites per gram of material (nn(T)) for LDH1, snomax, K-feldpar, quartz, cholesterol, snomax, birch pollen washing water, silver iodide (AgI), as plotted in Fig 5. Some data is taken from literature and references are provided where appropriate. fig6abc_tnuc-survival.xls: Contains well specific data for post-thaw viable cell number, survival compared to non-frozen baseline and ice nucleation temperature for the correlation experiment HepG2 96-well plate culture. Plotted in fig 6a,b,c. fig6d_welltemptimeseries.xlsx: contains thermal histories of each well (containing cyroprotectant aliquot and HepG2 cell monolayer) of the correlation experiment culture plate during cryopreservation run, as plotted in fig 6d. fig7_rawcelldata_immediate.xlsx: Contains the post-thaw viable cell assay results for the immediate post-thaw plate freezing trial, divided into ice-nucleation control technique (Icestart, manual nucleation and uncontrolled) for each plate. Plotted as boxplots in Fig 7a, example individual plate plotted in Fig 7c. fig7_rawcelldata5day.xlsx: Contains the post-thaw viable cell assay results for the 5-day post-thaw plate freezing trial, divided into ice-nucleation control technique (Icestart and uncontrolled) for each plate. Plotted as boxplots in Fig 7d, example individual plate plotted in Fig 7e. fig7a_anovaoutputs_immediatepostthaw.docx: Contains the outputs for the ANOVA significance testing for the immediate post-thaw trial cell viabilty data. Differences between groups within each plate (nucleation method) notated in Fig 7a. fig7b_averagesurvivalanova_immediatepost_thaw.docx: Contains the outputs for the ANOVA significance testing for the overall immediate post-thaw survival. Results and differences between groups plotted in Fig 7b fig7d_t-testoutputs_5daypostthaw.docx: Contains the outputs for the t-test significance testing for the 5-day post-thaw trial cell viabilty data. Differences between groups within each plate (nucleation method) notated in Fig 7d. fig7e_averagesurvivalt-test_5-daypostthaw.docx: Contains the outputs for the ANOVA significance testing for the overall 5-day post-thaw survival. Results and differences between groups plotted in Fig 7e 5. METHODS ---------- See main articles and/or references below for detailed methodologies: - ANOVA and t-test analysis was carried out on Minitab v17.20.2 - Droplet thermal histories were recorded using the IR-NIPI instrument (Harrison et al, 2018, https://doi.org/10.5194/amt-11-5629-2018) - Droplet freezing (nucleation) temperatures were recorded using the IR-NIPI or uL-NIPI cold-stages (Whale et al, 2015, https://doi.org/10.5194/amt-8-2437-2015)