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Dataset for 'A highly active mineral-based ice nucleating agent supports in situ cell cryopreservation in a high throughput format'
Citation
Daily, Martin I. and Whale, Thomas F. and Kilbride, Peter and Lamb, Stephen and Picton, Helen M. (2023) Dataset for 'A highly active mineral-based ice nucleating agent supports in situ cell cryopreservation in a high throughput format'. University of Leeds. [Dataset] https://doi.org/10.5518/1288
Dataset description
Cryopreservation of biological matter in microlitre scale volumes of liquid would be useful for a range of applications. At present, it is challenging because small volumes of water tend to supercool, and deep supercooling is known to lead to poor post thaw cell viability. Here we show that a mineral ice nucleator can almost eliminate supercooling in 100 microlitre liquid volumes during cryopreservation. This strategy of eliminating supercooling greatly enhances cell viability relative to cryopreservation protocols with uncontrolled ice nucleation. Using infrared thermography, we demonstrate a direct relationship between extent of supercooling and post-thaw cell viability. Using a mineral nucleator delivery system we open the door to the routine cryopreservation of mammalian cells in multiwell plates for applications such as high throughput toxicology testing of pharmaceutical products and regenerative medicine.
| Keywords: | cell cryopreservation ice-nucleation ice nucleation supercooling heterogeneous ice-nucleation ice nucleation toxicology screening multiwell plates | ||||||
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| Divisions: | Faculty of Environment > School of Earth and Environment | ||||||
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| License: | Creative Commons Attribution 4.0 International (CC BY 4.0) | ||||||
| Date deposited: | 08 Feb 2023 16:10 | ||||||
| URI: | https://archive.researchdata.leeds.ac.uk/id/eprint/1069 | ||||||
Files
Documentation
README_Daily_2023 [5kB]  |
README_Daily_2023 [5kB] 
README_Daily_2023 [5kB] Data
fig4a_water.xlsx [47kB] |
fig4bfghi_LDH1.xlsx [78kB] |
fig4c_cholesterol.xlsx [35kB] |
fig4d_snomax.xlsx [35kB] |
fig4e_k-felspar.xlsx [33kB] |
fig5_nmandfreezingtemperature.xlsx [41kB] |
fig6abc_tnuc-survival.xlsx [17kB] |
fig6d_welltemptimeseries.xlsx [42kB] |
fig7_rawcelldata_immediate.xlsx [24kB] |
fig7_rawcelldata5day.xlsx [24kB] |
fig7a_anovaoutputs_immediatepostthaw.docx [304kB] |
fig7b_averagesurvivalanova_immediatepost_thaw.docx [84kB] |
fig7d_t-testoutputs_5daypostthaw.docx [39kB] |
fig7e_averagesurvivalt-test_5-daypostthaw.docx [21kB] |