Research Data Leeds Repository

Dataset for a study correlating fluorescence quenching and topographic mapping of LHCII aggregates and lipid bilayers

Citation

Adams, Peter G. and Vasilev, Cvetelin and Hunter, C. Neil and Johnson, Matthew (2018) Dataset for a study correlating fluorescence quenching and topographic mapping of LHCII aggregates and lipid bilayers. University of Leeds. [Dataset] https://doi.org/10.5518/313¹

Dataset description

This dataset shows the raw data, analysed data and documentation related to figures and tables from the study "Correlated fluorescence quenching and topographic mapping of Light-Harvesting Complex II within surface-assembled aggregates and lipid bilayers". This includes: absorbance and fluorescence spectra; images from atomic force microscopy (AFM) and fluorescence microscopy; analysis of fitting of fluorescence spectroscopy data (FLIM); calculation of excitation power.

Keywords:	Light harvesting, Light-Harvesting Complex II (LHCII), photosynthesis, non-photochemical quenching (NPQ), time-resolved fluorescence, atomic force microscopy
Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C770 - Biophysical science2 C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C750 - Plant biochemistry3 C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C710 - Applied molecular biology, biophysics & biochemistry4
Divisions:	Faculty of Biological Sciences > Astbury Centre for Structural Molecular Biology5 Faculty of Engineering and Physical Sciences > School of Physics and Astronomy6
Related resources:	Location Type https://doi.org/10.1016/j.bbabio.2018.06.0117 Publication https://eprints.whiterose.ac.uk/134944/8 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	19 Jun 2018 09:02
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/3779
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Adams, Peter G. https://orcid.org/0000-0002-3940-877010 University of Leeds https://ror.org/024mrxd33 ror Vasilev, Cvetelin https://orcid.org/0000-0002-0536-882X11 University of Sheffield https://ror.org/05krs5044 ror Hunter, C. Neil https://orcid.org/0000-0003-2533-978312 University of Sheffield https://ror.org/05krs5044 ror Johnson, Matthew University of Sheffield https://ror.org/05krs5044 ror Type of data: Dataset Research funders: BBSRC, U.S. Department of Energy, BBSRC, ERC, U.S. Department of Energy, BBSRC, Leverhulme Trust, Human Frontiers Science Programme Grant numbers: BB/M013723/1 Publication date: 2018 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: p.g.adams@leeds.ac.uk13 Last Modified: 27 Jan 2025 15:16

Files

Documentation

Readme to access data [536B] 14 Readme to access data [536B] 15 Readme to access data [536B] 16 Security: Anyone File name: README.txt17 Content type: Documentation Description: Instructions on how to open the other data files Mime-Type: text/plain File size: 536B

File listing of AFM.zip [4kB] 18 File listing of AFM.zip [4kB] 19 File listing of AFM.zip [4kB] 20 Security: Anyone File name: AFM.zip.manifest.txt21 Content type: Documentation Mime-Type: text/plain File size: 4kB

Data

Spectroscopy data (cuvette-based) [590kB] 22 Spectroscopy data (cuvette-based) [590kB] 23 Spectroscopy data (cuvette-based) [590kB] 24 Security: Anyone File name: Spectroscopy_cuvette-based_Absorption-and-fluorescence-of-LHCII.opj25 Content type: Data Description: Absorption and fluorescence spectrscopy of LHCII in detergent suspension File size: 590kB

Spectroscopy data (FLIM) [21MB] 26 Spectroscopy data (FLIM) [21MB] 27 Spectroscopy data (FLIM) [21MB] 28 Security: Anyone File name: Spectroscopy_all_FLIM_data.opj29 Content type: Data Description: All fluorescence decay curves and fluorescence emission curves from LHCII samples on mica measured by FLIM (and controls). File size: 21MB

Fluorescence imaging data [464kB] 30 Fluorescence imaging data [464kB] 31 Fluorescence imaging data [464kB] 32 Security: Anyone File name: Flourescence_imaging_data.zip33 Content type: Data Description: Representative fluorescence image acquired on the FLIM of LHCII after thylakoid liposomes incubation on mica Mime-Type: application/zip File size: 464kB

Excitation energy calculations for FLIM [15kB] 34 Excitation energy calculations for FLIM [15kB] 35 Excitation energy calculations for FLIM [15kB] 36 Security: Anyone File name: Excitation_energy_calculations.xlsx37 Content type: Data Description: Spreadsheet for calculation of excitation energy for FLIM Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 15kB

Analysis of fluorescence lifetimes from FLIM [79kB] 38 Analysis of fluorescence lifetimes from FLIM [79kB] 39 Analysis of fluorescence lifetimes from FLIM [79kB] 40 Security: Anyone File name: Analysis_FLIM_lifetimes.xlsx41 Content type: Data Description: Analysis of fluorescence lifetime data. Spreadsheet showing all fits of all fluorescence decay curve raw data used in Table 1 in publication and further controls. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 79kB

AFM [321MB] 42 AFM [321MB] 43 AFM [321MB] 44 Security: Anyone File name: AFM.zip45 Content type: Data Description: Representative raw and exported AFM data of LHCII on mica - aggregates and LHCII in membranes following liposome incubation. Mime-Type: application/zip File size: 321MB

• 1. https://doi.org/10.5518/313
• 2. https://archive.researchdata.leeds.ac.uk/view/subjects/C770.html
• 3. https://archive.researchdata.leeds.ac.uk/view/subjects/C750.html
• 4. https://archive.researchdata.leeds.ac.uk/view/subjects/C710.html
• 5. https://archive.researchdata.leeds.ac.uk/view/divisions/ACSMB/
• 6. https://archive.researchdata.leeds.ac.uk/view/divisions/SPA/
• 7. https://doi.org/10.1016/j.bbabio.2018.06.011
• 8. https://eprints.whiterose.ac.uk/134944/
• 9. https://archive.researchdata.leeds.ac.uk/id/eprint/377
• 10. https://orcid.org/0000-0002-3940-8770
• 11. https://orcid.org/0000-0002-0536-882X
• 12. https://orcid.org/0000-0003-2533-9783
• 13. mailto:p.g.adams@leeds.ac.uk
• 14. https://archive.researchdata.leeds.ac.uk/377/3/README.txt
• 15. https://archive.researchdata.leeds.ac.uk/377/3/README.txt
• 16. https://archive.researchdata.leeds.ac.uk/377/3/README.txt
• 17. https://archive.researchdata.leeds.ac.uk/377/3/README.txt
• 18. https://archive.researchdata.leeds.ac.uk/377/9/AFM.zip.manifest.txt
• 19. https://archive.researchdata.leeds.ac.uk/377/9/AFM.zip.manifest.txt
• 20. https://archive.researchdata.leeds.ac.uk/377/9/AFM.zip.manifest.txt
• 21. https://archive.researchdata.leeds.ac.uk/377/9/AFM.zip.manifest.txt
• 22. https://archive.researchdata.leeds.ac.uk/377/1/Spectroscopy_cuvette-based_Absorption-and-fluorescence-of-LHCII.opj
• 23. https://archive.researchdata.leeds.ac.uk/377/1/Spectroscopy_cuvette-based_Absorption-and-fluorescence-of-LHCII.opj
• 24. https://archive.researchdata.leeds.ac.uk/377/1/Spectroscopy_cuvette-based_Absorption-and-fluorescence-of-LHCII.opj
• 25. https://archive.researchdata.leeds.ac.uk/377/1/Spectroscopy_cuvette-based_Absorption-and-fluorescence-of-LHCII.opj
• 26. https://archive.researchdata.leeds.ac.uk/377/2/Spectroscopy_all_FLIM_data.opj
• 27. https://archive.researchdata.leeds.ac.uk/377/2/Spectroscopy_all_FLIM_data.opj
• 28. https://archive.researchdata.leeds.ac.uk/377/2/Spectroscopy_all_FLIM_data.opj
• 29. https://archive.researchdata.leeds.ac.uk/377/2/Spectroscopy_all_FLIM_data.opj
• 30. https://archive.researchdata.leeds.ac.uk/377/4/Flourescence_imaging_data.zip
• 31. https://archive.researchdata.leeds.ac.uk/377/4/Flourescence_imaging_data.zip
• 32. https://archive.researchdata.leeds.ac.uk/377/4/Flourescence_imaging_data.zip
• 33. https://archive.researchdata.leeds.ac.uk/377/4/Flourescence_imaging_data.zip
• 34. https://archive.researchdata.leeds.ac.uk/377/5/Excitation_energy_calculations.xlsx
• 35. https://archive.researchdata.leeds.ac.uk/377/5/Excitation_energy_calculations.xlsx
• 36. https://archive.researchdata.leeds.ac.uk/377/5/Excitation_energy_calculations.xlsx
• 37. https://archive.researchdata.leeds.ac.uk/377/5/Excitation_energy_calculations.xlsx
• 38. https://archive.researchdata.leeds.ac.uk/377/6/Analysis_FLIM_lifetimes.xlsx
• 39. https://archive.researchdata.leeds.ac.uk/377/6/Analysis_FLIM_lifetimes.xlsx
• 40. https://archive.researchdata.leeds.ac.uk/377/6/Analysis_FLIM_lifetimes.xlsx
• 41. https://archive.researchdata.leeds.ac.uk/377/6/Analysis_FLIM_lifetimes.xlsx
• 42. https://archive.researchdata.leeds.ac.uk/377/7/AFM.zip
• 43. https://archive.researchdata.leeds.ac.uk/377/7/AFM.zip
• 44. https://archive.researchdata.leeds.ac.uk/377/7/AFM.zip
• 45. https://archive.researchdata.leeds.ac.uk/377/7/AFM.zip