Research Data Leeds Repository

Citation

Dewison, Katherine and Radford, Sheena E. (2025) Mapping the Role of Monomer Conformation in the Amyloid Formation of α-Synuclein Splice Variants. University of Leeds. [Dataset] https://doi.org/10.5518/1695

Dataset description

Dataset for 'Mapping the Role of Monomer Conformation in the Amyloid Formation of α-Synuclein Splice Variants'. Containing raw data including thioflavin T fluorescence data, percentages of insoluble material, SDS-PAGE gel images, and simulation trajectories.

Keywords:	Ionic strength; monomers; nanofibers; nucleation; peptides and proteins
Divisions:	Faculty of Biological Sciences > School of Molecular and Cellular Biology
Related resources:	Location Type https://doi.org/10.1021/jacs.5c12366 Publication https://eprints.whiterose.ac.uk/id/eprint/233284/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	04 Nov 2025 13:48
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/1485
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Dewison, Katherine https://orcid.org/0000-0002-7196-7687 University of Leeds https://ror.org/024mrxd33 ror Radford, Sheena E. https://orcid.org/0000-0002-3079-8039 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: Wellcome Trust, MRC, BBSRC, Royal Society Grant numbers: Wellcome (094232/Z/10/Z); BBSRC (BB/Z516053/1); MRC (MR/N013840/1); Wellcome (204963); Royal Society (RSRP/R1/211057) Publication date: 24 October 2025 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: fbskdew@leeds.ac.uk Last Modified: 04 Nov 2025 14:39

Files

Documentation

readme_Dewison_JACS_2025.txt [3kB] readme_Dewison_JACS_2025.txt [3kB] readme_Dewison_JACS_2025.txt [3kB] Security: Anyone File name: readme_Dewison_JACS_2025.txt Content type: Documentation Mime-Type: text/plain File size: 3kB

Data

De novo fibril formation at different protein concentrations for the alternative splice variants of αSyn (Figure 2) [459kB] De novo fibril formation at different protein concentrations for the alternative splice variants of αSyn (Figure 2) [459kB] De novo fibril formation at different protein concentrations for the alternative splice variants of αSyn (Figure 2) [459kB] Security: Anyone File name: Figure2.xlsx Content type: Data Description: Fig 2a - De novo ThT fluorescence data; Fig 2b - T50 values; and Fig 2c - percentages of insoluble material Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 459kB

Cross-seeding of the alternative splice variants of αSyn with αSyn monomer (Figure 3) [77kB] Cross-seeding of the alternative splice variants of αSyn with αSyn monomer (Figure 3) [77kB] Cross-seeding of the alternative splice variants of αSyn with αSyn monomer (Figure 3) [77kB] Security: Anyone File name: Figure3.xlsx Content type: Data Description: Fig 3a - Cross-seeded ThT fluorescence data; Fig 3b - percentages of insoluble material Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 77kB

Ionic strength dependence of amyloid formation of αSyn and its splice variants (Figure 4) [495kB] Ionic strength dependence of amyloid formation of αSyn and its splice variants (Figure 4) [495kB] Ionic strength dependence of amyloid formation of αSyn and its splice variants (Figure 4) [495kB] Security: Anyone File name: Figure4.xlsx Content type: Data Description: Fig 4a - Ionic strength-dependent ThT fluorescence data; Fig 4b - derived kappa values; Fig 4c - percentages of insoluble material Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 495kB

Monomeric conformers of the alternative splice variants of αSyn (Figure 5) [21kB] Monomeric conformers of the alternative splice variants of αSyn (Figure 5) [21kB] Monomeric conformers of the alternative splice variants of αSyn (Figure 5) [21kB] Security: Anyone File name: Figure5.xlsx Content type: Data Description: Fig 5a - Rh values identified by FIDA Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 21kB

Inter-residue distances correlate with secondary nucleation in amyloid formation (Figure 7) [58kB] Inter-residue distances correlate with secondary nucleation in amyloid formation (Figure 7) [58kB] Inter-residue distances correlate with secondary nucleation in amyloid formation (Figure 7) [58kB] Security: Anyone File name: Figure7.xlsx Content type: Data Description: Fig 7a - Spearman’s rank heatmap of the inter-residue distances between all residue pairs of the alternative splice variants of αSyn from CALVADOS 2 simulations with experimentally derived κ values Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 58kB

SDS-PAGE gels [98MB] SDS-PAGE gels [98MB] SDS-PAGE gels [98MB] Security: Anyone File name: SDS-PAGE.zip Content type: Data Description: Images of the SDS-PAGE gels used for densitometric analysis throughout the manuscript Mime-Type: application/zip File size: 98MB

De novo fibril formation assays for αSynΔ5 and αSynΔ3Δ5 at initial starting monomer concentrations of 2.5 μM to 20 μM [85kB] De novo fibril formation assays for αSynΔ5 and αSynΔ3Δ5 at initial starting monomer concentrations of 2.5 μM to 20 μM [85kB] De novo fibril formation assays for αSynΔ5 and αSynΔ3Δ5 at initial starting monomer concentrations of 2.5 μM to 20 μM [85kB] Security: Anyone File name: FigureS2.xlsx Content type: Data Description: Fig S2a - De novo ThT fluorescence at low monomer concentration; S2b - T50 values Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 85kB

Self-seeding of the alternative splice variants of αSyn [63kB] Self-seeding of the alternative splice variants of αSyn [63kB] Self-seeding of the alternative splice variants of αSyn [63kB] Security: Anyone File name: FigureS3.xlsx Content type: Data Description: Fig S3a - Self-seeding ThT fluorescence data; percentages of insoluble material from self-seeding assays Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 63kB

λ values for the splice variants of αSyn across a range of ionic strengths [25kB] λ values for the splice variants of αSyn across a range of ionic strengths [25kB] λ values for the splice variants of αSyn across a range of ionic strengths [25kB] Security: Anyone File name: FigureS5.xlsx Content type: Data Description: Fig S5 - λ values derived from the ionic strength dependent ThT data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 25kB

Self-seeding using seeds from different points of the plateau phase [40kB] Self-seeding using seeds from different points of the plateau phase [40kB] Self-seeding using seeds from different points of the plateau phase [40kB] Security: Anyone File name: FigureS7.xlsx Content type: Data Description: Fig S7 - Self-seeding ThT flurorescence data using fibrils taken from different points of the plateau Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 40kB

Taylorgrams of the alternative splice variants at different ionic strengths [517kB] Taylorgrams of the alternative splice variants at different ionic strengths [517kB] Taylorgrams of the alternative splice variants at different ionic strengths [517kB] Security: Anyone File name: FigureS9.xlsx Content type: Data Description: Fig S9 - Representative Taylorgrams of the alternative splice variants under different ionic strengths Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 517kB

Contact probability maps for αSynFL, αSynΔ3, αSynΔ5, and αSynΔ3Δ5 at 49 mM ionic strength [392kB] Contact probability maps for αSynFL, αSynΔ3, αSynΔ5, and αSynΔ3Δ5 at 49 mM ionic strength [392kB] Contact probability maps for αSynFL, αSynΔ3, αSynΔ5, and αSynΔ3Δ5 at 49 mM ionic strength [392kB] Security: Anyone File name: FigureS10.xlsx Content type: Data Description: Fig S10 - Contact probability maps for the splice variants at 49 mM ionic strength Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 392kB

Contact probability maps for αSynFL, αSynΔ3, αSynΔ5, and αSynΔ3Δ5 at 453 mM ionic strength [383kB] Contact probability maps for αSynFL, αSynΔ3, αSynΔ5, and αSynΔ3Δ5 at 453 mM ionic strength [383kB] Contact probability maps for αSynFL, αSynΔ3, αSynΔ5, and αSynΔ3Δ5 at 453 mM ionic strength [383kB] Security: Anyone File name: FigureS11.xlsx Content type: Data Description: Fig S11 - Contact probability maps for the splice variants at 453 mM ionic strength Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet File size: 383kB

Simulations [193MB] Simulations [193MB] Simulations [193MB] Security: Anyone File name: Simulations.zip Content type: Data Description: Contains all of the CALVADOS 2 trajectories and the related analyses Mime-Type: application/zip File size: 193MB