1. ABOUT THE DATASET -------------------- Title: Data for "Mapping the Role of Monomer Conformation in the Amyloid Formation of α-Synuclein Splice Variants" Creator(s): Katherine M. Dewison, Alexander I. P. Taylor, David J. Brockwell*, Sheena E. Radford* Organisation(s): 1. University of Leeds Rights-holder(s):Unless otherwise stated, Copyright 2025 University of Leeds Publication Year: 2025 Description: Dataset for 'Mapping the Role of Monomer Conformation in the Amyloid Formation of α-Synuclein Splice Variants'. Containing raw data including thioflavin T fluorescence data, percentages of insoluble material, SDS-PAGE gel images, and simulation trajectories. Cite as: Dewison, K.M., Taylor, A. I. P., Brockwell, D. J., and Radford, S. E. (2025): Data for "Mapping the Role of Monomer Conformation in the Amyloid Formation of α-Synuclein Splice Variants". University of Leeds. [Dataset] https://doi.org/10.5518/1695 Related publication: Dewison, K.M., Taylor, A. I. P., Brockwell, D. J., and Radford, S. E. (2025) Mapping the Role of Monomer Conformation in the Amyloid Formation of α-Synuclein Splice Variants. JACS (accepted). Contact: Katherine M. Dewison (fbskdew@leeds.ac.uk), Sheena E. Radford (s.e.radford@leeds.ac.uk) 2. TERMS OF USE --------------- Unless otherwise stated, this dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/. 3. PROJECT AND FUNDING INFORMATION ---------------------------------- Title: Mapping the Role of Monomer Conformation in the Amyloid Formation of α-Synuclein Splice Variants Dates: N/A Funding organisation: Wellcome Trust, MRC, BBSRC, Royal Society Grant no.: Wellcome (094232/Z/10/Z); BBSRC (BB/Z516053/1); MRC (MR/N013840/1); Wellcome (204963); Royal Society (RSRP/R1/211057) 4. CONTENTS ----------- File listing: Figure2.xlsx : Fig 2a - De novo ThT fluorescence data; Fig 2b - T50 values; and Fig 2c - percentages of insoluble material Figure3.xlsx : Fig 3a - Cross-seeded ThT fluorescence data; Fig 3b - percentages of insoluble material Figure4.xlsx : Fig 4a - Ionic strength-dependent ThT fluorescence data; Fig 4b - derived kappa values; Fig 4c - percentages of insoluble material Figure5.xlsx : Fig 5a - Rh values identified by FIDA Figure7.xlsx : Fig 7a - Spearman’s rank heatmap of the inter-residue distances between all residue pairs of the alternative splice variants of αSyn from CALVADOS 2 simulations with experimentally derived κ values SDS-PAGE.zip : Images of the SDS-PAGE gels used for densitometric analysis throughout the manuscript FigureS2.xlsx : Fig S2a - De novo ThT fluorescence at low monomer concentration; S2b - T50 values FigureS3.xlsx : Fig S3a - Self-seeding ThT fluorescence data; percentages of insoluble material from self-seeding assays FigureS5.xlsx : Fig S5 - λ values derived from the ionic strength dependent ThT data FigureS7.xlsx : Fig S7 - Self-seeding ThT flurorescence data using fibrils taken from different points of the plateau FigureS9.xlsx : Fig S9 - Representative Taylorgrams of the alternative splice variants under different ionic strengths FigureS10.xlsx : Fig S10 - Contact probability maps for the splice variants at 49 mM ionic strength FigureS11.xlsx : Fig S11 - Contact probability maps for the splice variants at 453 mM ionic strength Simulations.zip : Contains all of the CALVADOS 2 trajectories and the related analyses 5. METHODS ---------- For full method details, see the associated publication or contact the corresponding authors.