Research Data Leeds Repository

Dataset for the study of Self-quenching behaviour of a fluorescent probe incorporated within lipid membranes explored using electrophoresis and fluorescence lifetime imaging microscopy

Citation

Meredith, Sophie A. and Kusunoki, Yuka and Connell, Simon D. and Morigaki, Kenichi and Evans, Stephen D. and Adams, Peter G. (2023) Dataset for the study of Self-quenching behaviour of a fluorescent probe incorporated within lipid membranes explored using electrophoresis and fluorescence lifetime imaging microscopy. University of Leeds. [Dataset] https://doi.org/10.5518/1284¹

Dataset description

This dataset shows the raw data, analysed data and documentation related to figures and tables from the study "Self-quenching behaviour of a fluorescent probes incorporated within lipid membranes explored using electrophoresis and fluorescence lifetime imaging microscopy". This includes: fluorescence decay curves and spectra; fluorescence microscopy images; analysis of fitting of fluorescence spectroscopy data (FLIM); associated calculations.

Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C770 - Biophysical science2 C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C710 - Applied molecular biology, biophysics & biochemistry3 F000 - Physical sciences > F100 - Chemistry > F170 - Physical chemistry4
Divisions:	Faculty of Biological Sciences > Astbury Centre for Structural Molecular Biology5 Faculty of Engineering and Physical Sciences > School of Physics and Astronomy6
Related resources:	Location Type https://doi.org/10.1021/acs.jpcb.2c076527 Publication https://eprints.whiterose.ac.uk/197097/8 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	17 Feb 2023 12:40
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/10719
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Meredith, Sophie A. https://orcid.org/0000-0003-2550-909210 University of Leeds https://ror.org/024mrxd33 ror Kusunoki, Yuka Kobe University https://ror.org/03tgsfw79 ror Connell, Simon D. https://orcid.org/0000-0003-2500-572411 University of Leeds https://ror.org/024mrxd33 ror Morigaki, Kenichi https://orcid.org/0000-0002-2454-651312 Kobe University https://ror.org/03tgsfw79 ror Evans, Stephen D. https://orcid.org/0000-0001-8342-533513 University of Leeds https://ror.org/024mrxd33 ror Adams, Peter G. https://orcid.org/0000-0002-3940-877014 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: BBSRC, Japan Society for the Promotion of Science (JSPS), EPSRC Grant numbers: BB/M011151/1, JPJSBP120195707; 19H04725; 21KK0088, EP/R043337/1; EP/R03608X/1; EP/J017566/1, EP/T013958/1; BB/R000174/1 Publication date: 2023 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: p.g.adams@leeds.ac.uk15 Last Modified: 07 Jun 2024 12:23

Files

Documentation

README - Meredith 2023 [4kB] 16 README - Meredith 2023 [4kB] 17 README - Meredith 2023 [4kB] 18 Security: Anyone File name: README_Meredith_2023a.txt19 Content type: Documentation Description: Read me file which contains details about the dataset Mime-Type: text/plain File size: 4kB

Data

FLIM data control samples - SLBs with TR at defined conc [6MB] 20 FLIM data control samples - SLBs with TR at defined conc [6MB] 21 FLIM data control samples - SLBs with TR at defined conc [6MB] 22 Security: Anyone File name: FLIM_data_control_samples_SLBs_with_TR_at_defined_conc.zip23 Content type: Data Description: FLIM data related to an experiment on control samples to generate a standard curve (Figure 3 in the related published work). Raw data (.tif files) and exported images (.bmp files). Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 6MB

FLIM data - example images of showing membrane defects [859kB] 24 FLIM data - example images of showing membrane defects [859kB] 25 FLIM data - example images of showing membrane defects [859kB] 26 Security: Anyone File name: FLIM_data_example_images_of_showing_membrane_defects.zip27 Content type: Data Description: FLIM images (.bmp files) showing defects in membrane corrals (Figure S4 in the related published work). Mime-Type: application/zip File size: 859kB

FLIM data - kinetics of TR electrophoresis [10MB] 28 FLIM data - kinetics of TR electrophoresis [10MB] 29 FLIM data - kinetics of TR electrophoresis [10MB] 30 Security: Anyone File name: FLIM_data_kinetics_of_TR_electrophoresis.zip31 Content type: Data Description: FLIM data related to an experiment on the kinetics of TR migration during electrophoresis (Figure 2 in the related published work). Raw data (.tif files) and exported images (.bmp files). Mime-Type: application/zip File size: 10MB

FLIM data - TR concentration comparison - 0-28pc TR [4MB] 32 FLIM data - TR concentration comparison - 0-28pc TR [4MB] 33 FLIM data - TR concentration comparison - 0-28pc TR [4MB] 34 Security: Anyone File name: FLIM_data_TR_concentration_comparison_0-28pc_TR.zip35 Content type: Data Description: FLIM data related to an electrophoresis experiment where the TR concentration was 0.28% mol/mol (Figure 5 in the related published work). Raw data (.tif files) and exported images (.bmp files). Mime-Type: application/zip File size: 4MB

FLIM data - TR concentration comparison - 0-57pc TR [7MB] 36 FLIM data - TR concentration comparison - 0-57pc TR [7MB] 37 FLIM data - TR concentration comparison - 0-57pc TR [7MB] 38 Security: Anyone File name: FLIM_data_TR_concentration_comparison_0-57pc_TR.zip39 Content type: Data Description: FLIM data related to an electrophoresis experiment where the TR concentration was 0.57% mol/mol (Figure 5 in the related published work). Raw data (.tif files) and exported images (.bmp files). Mime-Type: application/zip File size: 7MB

FLIM data - TR concentration comparison - 0-85pc TR [4MB] 40 FLIM data - TR concentration comparison - 0-85pc TR [4MB] 41 FLIM data - TR concentration comparison - 0-85pc TR [4MB] 42 Security: Anyone File name: FLIM_data_TR_concentration_comparison_0-85pc_TR.zip43 Content type: Data Description: FLIM data related to an electrophoresis experiment where the TR concentration was 0.85% mol/mol (Figure 5 in the related published work). Raw data (.tif files) and exported images (.bmp files). Mime-Type: application/zip File size: 4MB

FLIM analysis - control sample calibration curve [157kB] 44 FLIM analysis - control sample calibration curve [157kB] 45 FLIM analysis - control sample calibration curve [157kB] 46 Security: Anyone File name: FLIM_analysis_control_sample_calibration_curve.opju47 Content type: Data Description: Processed FLIM data where an analysis of fluroescence versus concentration was performed File size: 157kB

FLIM analysis - kinetics of TR electrophoresis [971kB] 48 FLIM analysis - kinetics of TR electrophoresis [971kB] 49 FLIM analysis - kinetics of TR electrophoresis [971kB] 50 Security: Anyone File name: FLIM_analysis_kinetics_of_TR_electrophoresis.opju51 Content type: Data Description: Processed FLIM data where an analysis of the kinetics of TR electrophoresis was performed File size: 971kB

FLIM analysis - TR concentration comparison - ALL [7MB] 52 FLIM analysis - TR concentration comparison - ALL [7MB] 53 FLIM analysis - TR concentration comparison - ALL [7MB] 54 Security: Anyone File name: FLIM_analysis_TR_concentration_comparison_ALL.opju55 Content type: Data Description: Processed FLIM data including the analysis of several experiments related to TR electrophoresis and TR concentration (Figures 3-6 in the related published work) File size: 7MB

FLIM analysis - TR decay curve fit analysis [1MB] 56 FLIM analysis - TR decay curve fit analysis [1MB] 57 FLIM analysis - TR decay curve fit analysis [1MB] 58 Security: Anyone File name: FLIM_analysis_TR_decay_curve_fit_analysis.opju59 Content type: Data Description: Processed FLIM data where an analysis of fluorescence decay curves was performed File size: 1MB

• 1. https://doi.org/10.5518/1284
• 2. https://archive.researchdata.leeds.ac.uk/view/subjects/C770.html
• 3. https://archive.researchdata.leeds.ac.uk/view/subjects/C710.html
• 4. https://archive.researchdata.leeds.ac.uk/view/subjects/F170.html
• 5. https://archive.researchdata.leeds.ac.uk/view/divisions/ACSMB/
• 6. https://archive.researchdata.leeds.ac.uk/view/divisions/SPA/
• 7. https://doi.org/10.1021/acs.jpcb.2c07652
• 8. https://eprints.whiterose.ac.uk/197097/
• 9. https://archive.researchdata.leeds.ac.uk/id/eprint/1071
• 10. https://orcid.org/0000-0003-2550-9092
• 11. https://orcid.org/0000-0003-2500-5724
• 12. https://orcid.org/0000-0002-2454-6513
• 13. https://orcid.org/0000-0001-8342-5335
• 14. https://orcid.org/0000-0002-3940-8770
• 15. mailto:p.g.adams@leeds.ac.uk
• 16. https://archive.researchdata.leeds.ac.uk/1071/11/README_Meredith_2023a.txt
• 17. https://archive.researchdata.leeds.ac.uk/1071/11/README_Meredith_2023a.txt
• 18. https://archive.researchdata.leeds.ac.uk/1071/11/README_Meredith_2023a.txt
• 19. https://archive.researchdata.leeds.ac.uk/1071/11/README_Meredith_2023a.txt
• 20. https://archive.researchdata.leeds.ac.uk/1071/1/FLIM_data_control_samples_SLBs_with_TR_at_defined_conc.zip
• 21. https://archive.researchdata.leeds.ac.uk/1071/1/FLIM_data_control_samples_SLBs_with_TR_at_defined_conc.zip
• 22. https://archive.researchdata.leeds.ac.uk/1071/1/FLIM_data_control_samples_SLBs_with_TR_at_defined_conc.zip
• 23. https://archive.researchdata.leeds.ac.uk/1071/1/FLIM_data_control_samples_SLBs_with_TR_at_defined_conc.zip
• 24. https://archive.researchdata.leeds.ac.uk/1071/2/FLIM_data_example_images_of_showing_membrane_defects.zip
• 25. https://archive.researchdata.leeds.ac.uk/1071/2/FLIM_data_example_images_of_showing_membrane_defects.zip
• 26. https://archive.researchdata.leeds.ac.uk/1071/2/FLIM_data_example_images_of_showing_membrane_defects.zip
• 27. https://archive.researchdata.leeds.ac.uk/1071/2/FLIM_data_example_images_of_showing_membrane_defects.zip
• 28. https://archive.researchdata.leeds.ac.uk/1071/3/FLIM_data_kinetics_of_TR_electrophoresis.zip
• 29. https://archive.researchdata.leeds.ac.uk/1071/3/FLIM_data_kinetics_of_TR_electrophoresis.zip
• 30. https://archive.researchdata.leeds.ac.uk/1071/3/FLIM_data_kinetics_of_TR_electrophoresis.zip
• 31. https://archive.researchdata.leeds.ac.uk/1071/3/FLIM_data_kinetics_of_TR_electrophoresis.zip
• 32. https://archive.researchdata.leeds.ac.uk/1071/4/FLIM_data_TR_concentration_comparison_0-28pc_TR.zip
• 33. https://archive.researchdata.leeds.ac.uk/1071/4/FLIM_data_TR_concentration_comparison_0-28pc_TR.zip
• 34. https://archive.researchdata.leeds.ac.uk/1071/4/FLIM_data_TR_concentration_comparison_0-28pc_TR.zip
• 35. https://archive.researchdata.leeds.ac.uk/1071/4/FLIM_data_TR_concentration_comparison_0-28pc_TR.zip
• 36. https://archive.researchdata.leeds.ac.uk/1071/5/FLIM_data_TR_concentration_comparison_0-57pc_TR.zip
• 37. https://archive.researchdata.leeds.ac.uk/1071/5/FLIM_data_TR_concentration_comparison_0-57pc_TR.zip
• 38. https://archive.researchdata.leeds.ac.uk/1071/5/FLIM_data_TR_concentration_comparison_0-57pc_TR.zip
• 39. https://archive.researchdata.leeds.ac.uk/1071/5/FLIM_data_TR_concentration_comparison_0-57pc_TR.zip
• 40. https://archive.researchdata.leeds.ac.uk/1071/6/FLIM_data_TR_concentration_comparison_0-85pc_TR.zip
• 41. https://archive.researchdata.leeds.ac.uk/1071/6/FLIM_data_TR_concentration_comparison_0-85pc_TR.zip
• 42. https://archive.researchdata.leeds.ac.uk/1071/6/FLIM_data_TR_concentration_comparison_0-85pc_TR.zip
• 43. https://archive.researchdata.leeds.ac.uk/1071/6/FLIM_data_TR_concentration_comparison_0-85pc_TR.zip
• 44. https://archive.researchdata.leeds.ac.uk/1071/7/FLIM_analysis_control_sample_calibration_curve.opju
• 45. https://archive.researchdata.leeds.ac.uk/1071/7/FLIM_analysis_control_sample_calibration_curve.opju
• 46. https://archive.researchdata.leeds.ac.uk/1071/7/FLIM_analysis_control_sample_calibration_curve.opju
• 47. https://archive.researchdata.leeds.ac.uk/1071/7/FLIM_analysis_control_sample_calibration_curve.opju
• 48. https://archive.researchdata.leeds.ac.uk/1071/8/FLIM_analysis_kinetics_of_TR_electrophoresis.opju
• 49. https://archive.researchdata.leeds.ac.uk/1071/8/FLIM_analysis_kinetics_of_TR_electrophoresis.opju
• 50. https://archive.researchdata.leeds.ac.uk/1071/8/FLIM_analysis_kinetics_of_TR_electrophoresis.opju
• 51. https://archive.researchdata.leeds.ac.uk/1071/8/FLIM_analysis_kinetics_of_TR_electrophoresis.opju
• 52. https://archive.researchdata.leeds.ac.uk/1071/9/FLIM_analysis_TR_concentration_comparison_ALL.opju
• 53. https://archive.researchdata.leeds.ac.uk/1071/9/FLIM_analysis_TR_concentration_comparison_ALL.opju
• 54. https://archive.researchdata.leeds.ac.uk/1071/9/FLIM_analysis_TR_concentration_comparison_ALL.opju
• 55. https://archive.researchdata.leeds.ac.uk/1071/9/FLIM_analysis_TR_concentration_comparison_ALL.opju
• 56. https://archive.researchdata.leeds.ac.uk/1071/10/FLIM_analysis_TR_decay_curve_fit_analysis.opju
• 57. https://archive.researchdata.leeds.ac.uk/1071/10/FLIM_analysis_TR_decay_curve_fit_analysis.opju
• 58. https://archive.researchdata.leeds.ac.uk/1071/10/FLIM_analysis_TR_decay_curve_fit_analysis.opju
• 59. https://archive.researchdata.leeds.ac.uk/1071/10/FLIM_analysis_TR_decay_curve_fit_analysis.opju