Research Data Leeds Repository

FPR1 photoaffinity probe - associated data

Citation

Wright, Megan and Warriner, Stuart L. and Field, Devon and White, Jack (2023) FPR1 photoaffinity probe - associated data. University of Leeds. [Dataset] https://doi.org/10.5518/1226¹

Dataset description

NMR spectroscopy data from the synthesis of fmoc-photomethionine, HPLC traces for the synthesis of peptidic fluorescent probes for formylpeptide receptor 1, and SDS-PAGE gel electrophoresis data, flow cytometry data and microscopy data for analysis of probe binding to proteins.

Keywords:	chemical biology
Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C720 - Biological chemistry2 F000 - Physical sciences > F100 - Chemistry3 F000 - Physical sciences > F100 - Chemistry > F160 - Organic chemistry4 F000 - Physical sciences > F100 - Chemistry > F160 - Organic chemistry > F163 - Bio-organic chemistry5 F000 - Physical sciences > F100 - Chemistry > F160 - Organic chemistry > F165 - Biomolecular chemistry6 C000 - Biological sciences > C100 - Biology > C130 - Cell biology7 C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry8
Divisions:	Faculty of Engineering and Physical Sciences > School of Chemistry9
Related resources:	Location Type https://doi.org/10.1039/D2CB00199C10 Publication https://eprints.whiterose.ac.uk/195435/11 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	23 Aug 2023 15:21
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/115112
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Wright, Megan https://orcid.org/0000-0003-2731-470713 University of Leeds https://ror.org/024mrxd33 ror Warriner, Stuart L. https://orcid.org/0000-0002-7589-601114 University of Leeds https://ror.org/024mrxd33 ror Field, Devon University of Leeds https://ror.org/024mrxd33 ror White, Jack University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: EPSRC, Royal Society, Leverhulme Trust Grant numbers: 1930246, RG160914, RPG-2018-030 Publication date: 2023 Resource language: English Metadata language: English Research project title: A chemical approach to unravelling how peptide signals mediate cell-cell communication, Novel photoaffinity probes to explore ligand binding to formyl peptide receptors Publisher: University of Leeds Contact email: m.h.wright@leeds.ac.uk15 Last Modified: 03 Sep 2024 13:15

Files

Documentation

README_Wright_2023.txt [2kB] 16 README_Wright_2023.txt [2kB] 17 README_Wright_2023.txt [2kB] 18 Security: Anyone File name: README_Wright_2023.txt19 Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2kB

Data

NMR data [222kB] 20 NMR data [222kB] 21 NMR data [222kB] 22 Security: Anyone File name: NMR_data.zip23 Content type: Data Description: Nuclear magnetic spectroscopy data for PhotoMet synthesis (pdf) Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 222kB

HPLC data [74kB] 24 HPLC data [74kB] 25 HPLC data [74kB] 26 Security: Anyone File name: HPLC_data.zip27 Content type: Data Description: Analytical High performance liquid chromatography data for tracer-FITC and probe_TAMRA (excel file) Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 74kB

SDS-PAGE data [10MB] 28 SDS-PAGE data [10MB] 29 SDS-PAGE data [10MB] 30 Security: Anyone File name: SDS_PAGE_data.zip31 Content type: Data Description: SDS-polyacrylamide gel electrophoresis data for Figs.4a, 4c, S1, S3, S4, S5, S6, S7 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 10MB

flow cytometry data [117MB] 32 flow cytometry data [117MB] 33 flow cytometry data [117MB] 34 Security: Anyone File name: FCS_data.zip35 Content type: Data Description: Flow cytometry data for Fig.2, 4 and S2 (as .fcs, .pdf files) Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 117MB

Microsopy data [1MB] 36 Microsopy data [1MB] 37 Microsopy data [1MB] 38 Security: Anyone File name: Microscopy_data.zip39 Content type: Data Description: Fluorescence microscopy data for Fig. 3 as jpg files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

• 1. https://doi.org/10.5518/1226
• 2. https://archive.researchdata.leeds.ac.uk/view/subjects/C720.html
• 3. https://archive.researchdata.leeds.ac.uk/view/subjects/F100.html
• 4. https://archive.researchdata.leeds.ac.uk/view/subjects/F160.html
• 5. https://archive.researchdata.leeds.ac.uk/view/subjects/F163.html
• 6. https://archive.researchdata.leeds.ac.uk/view/subjects/F165.html
• 7. https://archive.researchdata.leeds.ac.uk/view/subjects/C130.html
• 8. https://archive.researchdata.leeds.ac.uk/view/subjects/C700.html
• 9. https://archive.researchdata.leeds.ac.uk/view/divisions/CHEM/
• 10. https://doi.org/10.1039/D2CB00199C
• 11. https://eprints.whiterose.ac.uk/195435/
• 12. https://archive.researchdata.leeds.ac.uk/id/eprint/1151
• 13. https://orcid.org/0000-0003-2731-4707
• 14. https://orcid.org/0000-0002-7589-6011
• 15. mailto:m.h.wright@leeds.ac.uk
• 16. https://archive.researchdata.leeds.ac.uk/1151/6/README_Wright_2023.txt
• 17. https://archive.researchdata.leeds.ac.uk/1151/6/README_Wright_2023.txt
• 18. https://archive.researchdata.leeds.ac.uk/1151/6/README_Wright_2023.txt
• 19. https://archive.researchdata.leeds.ac.uk/1151/6/README_Wright_2023.txt
• 20. https://archive.researchdata.leeds.ac.uk/1151/1/NMR_data.zip
• 21. https://archive.researchdata.leeds.ac.uk/1151/1/NMR_data.zip
• 22. https://archive.researchdata.leeds.ac.uk/1151/1/NMR_data.zip
• 23. https://archive.researchdata.leeds.ac.uk/1151/1/NMR_data.zip
• 24. https://archive.researchdata.leeds.ac.uk/1151/2/HPLC_data.zip
• 25. https://archive.researchdata.leeds.ac.uk/1151/2/HPLC_data.zip
• 26. https://archive.researchdata.leeds.ac.uk/1151/2/HPLC_data.zip
• 27. https://archive.researchdata.leeds.ac.uk/1151/2/HPLC_data.zip
• 28. https://archive.researchdata.leeds.ac.uk/1151/3/SDS_PAGE_data.zip
• 29. https://archive.researchdata.leeds.ac.uk/1151/3/SDS_PAGE_data.zip
• 30. https://archive.researchdata.leeds.ac.uk/1151/3/SDS_PAGE_data.zip
• 31. https://archive.researchdata.leeds.ac.uk/1151/3/SDS_PAGE_data.zip
• 32. https://archive.researchdata.leeds.ac.uk/1151/4/FCS_data.zip
• 33. https://archive.researchdata.leeds.ac.uk/1151/4/FCS_data.zip
• 34. https://archive.researchdata.leeds.ac.uk/1151/4/FCS_data.zip
• 35. https://archive.researchdata.leeds.ac.uk/1151/4/FCS_data.zip
• 36. https://archive.researchdata.leeds.ac.uk/1151/5/Microscopy_data.zip
• 37. https://archive.researchdata.leeds.ac.uk/1151/5/Microscopy_data.zip
• 38. https://archive.researchdata.leeds.ac.uk/1151/5/Microscopy_data.zip
• 39. https://archive.researchdata.leeds.ac.uk/1151/5/Microscopy_data.zip