1. ABOUT THE DATASET
--------------------

Title:	FPR1 photoaffinity probe - associated data

Creator(s): Devon Field [1], Jack White [1], Stuart Warriner[1], Megan Wright[1]

Organisation(s): 1. University of Leeds.

Rights-holder(s):

Publication Year: 2023

Description: NMR spectroscopy data from the synthesis of fmoc-photomethionine, HPLC traces for the synthesis of peptidic fluorescent probes for formylpeptide receptor 1, and SDS-PAGE gel electrophoresis data, flow cytometry data and microscopy data for analysis of probe binding to proteins.

Cite as: Devon Field, Jack White, Stuart Warriner, Megan Wright (2023) FPR1 photoaffinity probe. University of Leeds. [Dataset] https://doi.org/10.5518/1226

Related publication: Devon Field, Jack White, Stuart Warriner, Megan Wright (2023) A fluorescent photoaffinity probe for formyl peptide receptor 1 labelling in living cells. RSC Chemical Biology. doi: https://doi.org/10.1039/D2CB00199C

Contact: m.h.wright@leeds.ac.uk


2. TERMS OF USE
---------------

This dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/


3. PROJECT AND FUNDING INFORMATION
----------------------------------

Title: 

Dates: 

Funding organisation: EPSRC, Royal Society, Leverhulme Trust

Grant no.:

EPSRC DTA studentship to DF 1930246
Royal Society research Grant to MW RG160914
Leverhulme Trust Research Grant to JW RPG-2018-030


4. CONTENTS
-----------
File listing

NMR data.zip: pdf files showing 1H NMR spectra for compounds 1-7 (see related publication in section 1 above for structures).

HPLC data.zip: Analytical High performance liquid chromatography data for tracer-FITC and probe-TAMRA (excel file)

SDS PAGE data.zip: SDS-polyacrylamide gel electrophoresis data for Figs. 4a, 4c, S1, S3, S4, S5, S6, S7 (see related publication) as tif files

Flow cytometry data.zip: Flow cytometry data for Figs. 2, 4 and S2 (as .fcs, .pdf files)

Microscopy data.zip: Fluorescence microscopy data for Fig. 3 as jpg files


5. METHODS
----------

Detailed information about methods is available in the related publication in Section 1
above.