Research Data Leeds Repository

Citation

Xu, Yong and Maya-Martinez, Roberto and Guthertz, Nicolas and Heath, George R. and Manfield, Iain W. and Breeze, Alexander L. and Sobott, Frank and Foster, Richard and Radford, Sheena E. (2022) Supporting data for "Tuning the rate of aggregation of hIAPP into amyloid using small-molecule modulators of assembly". University of Leeds. [Dataset] https://doi.org/10.5518/1000

Dataset description

Human islet amyloid polypeptide (hIAPP) self-assembles into amyloid fibrils which deposit in pancreatic islets of type 2 diabetes (T2D) patients. Here, we applied chemical kinetics to study the mechanism of amyloid assembly of wild-type hIAPP and its more amyloidogenic natural variant S20G. We also report the discovery of two structurally distinct small-molecule modulators of hIAPP assembly.

Keywords:	hIAPP aggregation, Chemical kinetics, modu
Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C790 - Molecular biology, biophysics & biochemistry not elsewhere classified
Divisions:	Faculty of Biological Sciences > Astbury Centre for Structural Molecular Biology
Related resources:	Location Type https://doi.org/10.1038/s41467-022-28660-7 Publication https://eprints.whiterose.ac.uk/183086/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	31 Jan 2022 07:43
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/921
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Xu, Yong https://orcid.org/0000-0002-8389-4120 University of Leeds https://ror.org/024mrxd33 ror Maya-Martinez, Roberto https://orcid.org/0000-0002-7371-8051 University of Leeds https://ror.org/024mrxd33 ror Guthertz, Nicolas https://orcid.org/0000-0001-7247-1176 University of Leeds https://ror.org/024mrxd33 ror Heath, George R. https://orcid.org/0000-0001-6431-2191 University of Leeds https://ror.org/024mrxd33 ror Manfield, Iain W. https://orcid.org/0000-0003-3765-0325 University of Leeds https://ror.org/024mrxd33 ror Breeze, Alexander L. https://orcid.org/0000-0001-9723-3350 University of Leeds https://ror.org/024mrxd33 ror Sobott, Frank https://orcid.org/0000-0001-9029-1865 University of Leeds https://ror.org/024mrxd33 ror Foster, Richard https://orcid.org/0000-0002-2361-3884 University of Leeds https://ror.org/024mrxd33 ror Radford, Sheena E. https://orcid.org/0000-0002-3079-8039 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: Wellcome Trust Grant numbers: 204963, 062164/Z/00/Z Publication date: 2022 Resource language: English Metadata language: English Research project title: Protein-Protein Interactions in the Early Stages of Amyloidosis Publisher: University of Leeds Contact email: S.E.Radford@leeds.ac.uk Last Modified: 28 Jun 2024 09:59

Files

Documentation

List of extra data files [8kB] List of extra data files [8kB] List of extra data files [8kB] Security: Anyone File name: List_of_extra_data_files.xlsx Content type: Documentation Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8kB

Readme.txt [575B] Readme.txt [575B] Readme.txt [575B] Security: Anyone File name: Readme.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 575B

Data

Concentration-dependent aggregation of WT and S20G [100kB] Concentration-dependent aggregation of WT and S20G [100kB] Concentration-dependent aggregation of WT and S20G [100kB] Security: Anyone File name: Concentration-dependent_aggregation_of_WT_and_S20G.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 100kB

Seeded experiment of WT and S20G [46kB] Seeded experiment of WT and S20G [46kB] Seeded experiment of WT and S20G [46kB] Security: Anyone File name: Seeded_experiment_of_WT_and_S20G.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 46kB

ThT kinetics of inhibitor [230kB] ThT kinetics of inhibitor [230kB] ThT kinetics of inhibitor [230kB] Security: Anyone File name: ThT_kinetics_of_inhibitor.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 230kB

ThT kinetics of accelerator [209kB] ThT kinetics of accelerator [209kB] ThT kinetics of accelerator [209kB] Security: Anyone File name: ThT_kinetics_of_accelerator.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 209kB

Fluorescence quencing [35kB] Fluorescence quencing [35kB] Fluorescence quencing [35kB] Security: Anyone File name: Fluorescence_quencing.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 35kB

SPR [613kB] SPR [613kB] SPR [613kB] Security: Anyone File name: SPR.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 613kB

pyrene fluorescence [52kB] pyrene fluorescence [52kB] pyrene fluorescence [52kB] Security: Anyone File name: pyrene_fluorescence.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 52kB

fibril interaction with modulators [27kB] fibril interaction with modulators [27kB] fibril interaction with modulators [27kB] Security: Anyone File name: fibril_interaction_with_modulators.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 27kB

Native MS data [26MB] Native MS data [26MB] Native MS data [26MB] Security: Anyone File name: Native_MS_data.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 26MB

CID MS data [37kB] CID MS data [37kB] CID MS data [37kB] Security: Anyone File name: CID_MS_data.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 37kB

Supplementary data files [453kB] Supplementary data files [453kB] Supplementary data files [453kB] Security: Anyone File name: Supplementary_data_files.zip Content type: Data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 453kB