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Data for Timelapse (movie) of single vesicles fluorescence change upon potential application

Mazurenko, Ievgen and Jeuken, Lars (2018) Data for Timelapse (movie) of single vesicles fluorescence change upon potential application. University of Leeds. [Dataset] https://doi.org/10.5518/442

Dataset description

This data represents a typical fluorescence image obtained when analysing proton-pumping activity of single enzymes reconstituted in the liposomes. The time-lapse fluorescence image was taken within a single 5 min experiment. It consists from 2 channels (470/535 nm and 410/535 nm) x 105 time points. The reductive potential was applied from 60 to 180 s.

Keywords: Microscopy, fluorescence, single liposomes, single enzyme, pH-sensitive dye, electrochemistry
Subjects: C000 - Biological sciences
C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry
C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C770 - Biophysical science
Divisions: Faculty of Biological Sciences > School of Biomedical Sciences
Related resources:
LocationType
https://www.jove.com/video/58896/single-liposome-measurements-for-study-proton-pumping-membranePublication
http://eprints.whiterose.ac.uk/136317/Publication
License: Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited: 23 Sep 2018 20:05
URI: https://archive.researchdata.leeds.ac.uk/id/eprint/424

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