This data represents a typical fluorescence image obtained when analysing proton-pumping activity of single enzymes reconstituted in the liposomes.
Cytochrome bo3 was reconstituted into the liposomes with massic content 0.1%.
The time-lapse fluorescence image was taken within a single 5 min experiment.
It consists from 2 channels (470/535 nm and 410/535 nm) x 105 time points. The reductive potential (-0.2V vs NHE) was applied from 60 to 180 s. 

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