Research Data Leeds Repository

Citation

Mazurenko, Ievgen and Jeuken, Lars J. C. (2018) Data for Timelapse (movie) of single vesicles fluorescence change upon potential application. University of Leeds. [Dataset] https://doi.org/10.5518/442

Dataset description

This data represents a typical fluorescence image obtained when analysing proton-pumping activity of single enzymes reconstituted in the liposomes. The time-lapse fluorescence image was taken within a single 5 min experiment. It consists from 2 channels (470/535 nm and 410/535 nm) x 105 time points. The reductive potential was applied from 60 to 180 s.

Keywords:	Microscopy, fluorescence, single liposomes, single enzyme, pH-sensitive dye, electrochemistry
Subjects:	C000 - Biological sciences C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C770 - Biophysical science
Divisions:	Faculty of Biological Sciences > School of Biomedical Sciences
Related resources:	Location Type https://doi.org/10.3791/58896 Publication https://eprints.whiterose.ac.uk/136317/ Publication https://doi.org/10.3791/58896-v Audiovisual
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	23 Sep 2018 20:05
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/424
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Mazurenko, Ievgen https://orcid.org/0000-0003-2563-3130 University of Leeds https://ror.org/024mrxd33 ror Jeuken, Lars J. C. https://orcid.org/0000-0001-7810-3964 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: BBSRC Grant numbers: BB/P005454/1 Publication date: 2018 Resource language: English Metadata language: English Research project title: Single liposome measurements for the study of proton-pumping membrane enzymes using a combination of electrochemistry and fluorescent microscopy Publisher: University of Leeds Contact email: i.mazurenko@leeds.ac.uk Last Modified: 11 Oct 2024 14:52

Files

Documentation

Readme [662B] Readme [662B] Readme [662B] Security: Anyone File name: readme.txt Content type: Documentation Description: Readme Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 662B

Data

Timelapse image [2GB] Timelapse image [2GB] Timelapse image [2GB] Security: Anyone File name: _1j_CA001.tif Content type: Data Description: 16-bit multichannel (C2T105) image Mime-Type: image/tiff License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2GB

List of timestamps [898B] List of timestamps [898B] List of timestamps [898B] Security: Anyone File name: _1j_CA001.csv Content type: Data Description: Exact timestamps of every T-channel of the image Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 898B