Research Data Leeds Repository

Citation

Helliwell, Jack and Thomas, Daniel and Papathanasiou, Vaia and Homer-Vanniasinkam, Shervanthi and Desai, Amisha and Jennings, Louise and Rooney, Paul and Kearney, John and Ingham, Eileen (2017) Low concentration SDS decellularised porcine dermis. University of Leeds. [Dataset] https://doi.org/10.5518/177

Dataset description

Data on the biological and biomechanical properties of porcine dermis before and after decellularisation and decellularised human dermis: biomechanical testing data (stress strain curves); DNA and hydroxyproline content; histology images, immunohistochemical staining for alpha Gal epitope, collagen IV and laminin images

Keywords:	Dermis, Decellularisation, Skin, Xenograft
Subjects:	H000 - Engineering > H100 - General engineering > H160 - Bioengineering, biomedical engineering & clinical engineering > H162 - Biomechanics (including fluid & solid mechanics) C000 - Biological sciences > C900 - Others in Biological Sciences > C910 - Applied biological sciences H000 - Engineering > H100 - General engineering > H160 - Bioengineering, biomedical engineering & clinical engineering H000 - Engineering > H100 - General engineering > H160 - Bioengineering, biomedical engineering & clinical engineering > H161 - Biomaterials B000 - Subjects allied to medicine > B800 - Medical technology H000 - Engineering > H100 - General engineering > H160 - Bioengineering, biomedical engineering & clinical engineering > H165 - Tissue engineering & regenerative medicine H000 - Engineering > H100 - General engineering > H160 - Bioengineering, biomedical engineering & clinical engineering > H168 - Medical devices & instrumentation
Divisions:	Faculty of Biological Sciences > Institute of Medical and Biological Engineering Faculty of Engineering and Physical Sciences > Institute of Medical and Biological Engineering Faculty of Engineering and Physical Sciences > School of Mechanical Engineering > Institute of Medical and Biological Engineering
Related resources:	Location Type https://doi.org/10.1177/2041731417724011 Publication https://eprints.whiterose.ac.uk/119218/ Publication
License:	Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
Date deposited:	26 Jul 2017 15:19
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/179
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Helliwell, Jack https://orcid.org/0000-0002-3579-0183 University of Leeds https://ror.org/024mrxd33 ror Thomas, Daniel https://orcid.org/0000-0001-5584-9114 University of Leeds https://ror.org/024mrxd33 ror Papathanasiou, Vaia Leeds General Infirmary https://ror.org/04hrjej96 ror Homer-Vanniasinkam, Shervanthi https://orcid.org/0000-0001-6234-5053 Leeds General Infirmary https://ror.org/04hrjej96 ror Desai, Amisha https://orcid.org/0000-0001-8611-9958 University of Leeds https://ror.org/024mrxd33 ror Jennings, Louise https://orcid.org/0000-0003-1446-4511 University of Leeds https://ror.org/024mrxd33 ror Rooney, Paul https://orcid.org/0000-0003-3724-5717 NHS Blood and Transplant https://ror.org/0227qpa16 ror Kearney, John NHS Blood and Transplant https://ror.org/0227qpa16 ror Ingham, Eileen https://orcid.org/0000-0002-9757-3045 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: Wolfson Foundation, Wellcome Trust, EPSRC Grant numbers: WT088908/z/09/z Publication date: 2017 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: e.ingham@leeds.ac.uk Last Modified: 02 Sep 2024 14:06

Files

Documentation

Read me [854B] Read me [854B] Read me [854B] Security: Anyone File name: Readme.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) File size: 854B

Data

DNA content [16kB] DNA content [16kB] DNA content [16kB] Security: Anyone File name: DNA_assay_data.xlsx Content type: Data Description: Raw data for analysis of DNA content of decellularised porcine dermis, decellularised human dermis and fresh porcine skin Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) File size: 16kB

Hydroxyproline content [41kB] Hydroxyproline content [41kB] Hydroxyproline content [41kB] Security: Anyone File name: Hydroxyproline_assay_data.xlsx Content type: Data Description: Raw data for hydroxyproline analysis of decellularised porcine dermis, decellularised human dermis and fresh porcine skin Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) File size: 41kB

Biomechanical data - decellularised porcine dermis [1MB] Biomechanical data - decellularised porcine dermis [1MB] Biomechanical data - decellularised porcine dermis [1MB] Security: Anyone File name: Decell_porcine_mechanical_test_data.xlsx Content type: Data Description: Data from uniaxial tensile testing of decellularised porcine dermis; stress strain curves Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) File size: 1MB

Biomechanical data - fresh porcine skin [2MB] Biomechanical data - fresh porcine skin [2MB] Biomechanical data - fresh porcine skin [2MB] Security: Anyone File name: Fresh_porcine_mechanical_test_data.xlsx Content type: Data Description: Data from uniaxial tensile testing of fresh porcine skin;stress strain curves Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) File size: 2MB

Biomechanical data - decellularised human dermis [1MB] Biomechanical data - decellularised human dermis [1MB] Biomechanical data - decellularised human dermis [1MB] Security: Anyone File name: Decell_human_mechanical_test_data.xlsx Content type: Data Description: Data from uniaxial tensile testing of decellularised human dermis; stress strain curves Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) File size: 1MB

Histology images [176MB] Histology images [176MB] Histology images [176MB] Security: Anyone File name: Histology_images.zip Content type: Data Description: Images of H&E, DAPI and Sirius Millers stained sections of decellularised porcine dermis, fresh porcine skin Mime-Type: application/zip License: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) File size: 176MB

Immunohistochemistry images [231MB] Immunohistochemistry images [231MB] Immunohistochemistry images [231MB] Security: Anyone File name: Immunohistochemistry_images.zip Content type: Data Description: Images of sections of decellularised porcine dermis and fresh porcine skin stained using antibodies to collagen IV and laminin Mime-Type: application/zip License: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) File size: 231MB

Alpha-Gal images [89MB] Alpha-Gal images [89MB] Alpha-Gal images [89MB] Security: Anyone File name: alpha_gal_images.zip Content type: Data Description: Images of sections of decellularised porcine dermis and fresh porcine skin stained using GSL Lectin to detect alpha-Gal (and controls). Mime-Type: application/zip License: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) File size: 89MB