Research Data Leeds Repository

FPR1 photoaffinity probe - associated data

Wright, Megan and Warriner, Stuart and Field (nee Legge), Devon and White, Jack (2023) FPR1 photoaffinity probe - associated data. University of Leeds. [Dataset] https://doi.org/10.5518/1226

Dataset description

NMR spectroscopy data from the synthesis of fmoc-photomethionine, HPLC traces for the synthesis of peptidic fluorescent probes for formylpeptide receptor 1, and SDS-PAGE gel electrophoresis data, flow cytometry data and microscopy data for analysis of probe binding to proteins.

Keywords:	chemical biology
Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C720 - Biological chemistry F000 - Physical sciences > F100 - Chemistry F000 - Physical sciences > F100 - Chemistry > F160 - Organic chemistry F000 - Physical sciences > F100 - Chemistry > F160 - Organic chemistry > F163 - Bio-organic chemistry F000 - Physical sciences > F100 - Chemistry > F160 - Organic chemistry > F165 - Biomolecular chemistry C000 - Biological sciences > C100 - Biology > C130 - Cell biology C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry
Divisions:	Faculty of Engineering and Physical Sciences > School of Chemistry
Related resources:	Location Type https://doi.org/10.1039/D2CB00199C Publication https://eprints.whiterose.ac.uk/195435/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	23 Aug 2023 15:21
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/1151
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Wright, Megan https://orcid.org/0000-0003-2731-4707 University of Leeds https://ror.org/024mrxd33 ror Warriner, Stuart https://orcid.org/0000-0002-7589-6011 University of Leeds https://ror.org/024mrxd33 ror Field (nee Legge), Devon University of Leeds https://ror.org/024mrxd33 ror White, Jack University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: EPSRC, Royal Society, Leverhulme Trust Grant numbers: 1930246, RG160914, RPG-2018-030 Publication date: 2023 Resource language: English Research project title: A chemical approach to unravelling how peptide signals mediate cell-cell communication, Novel photoaffinity probes to explore ligand binding to formyl peptide receptors Publisher: University of Leeds Contact Email Address: m.h.wright@leeds.ac.uk Last Modified: 31 Aug 2023 13:30

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Documentation

README_Wright_2023.txt [2kB] README_Wright_2023.txt [2kB] README_Wright_2023.txt [2kB] Visible to: Anyone File name: README_Wright_2023.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2kB

Data

NMR data [222kB] NMR data [222kB] NMR data [222kB] Visible to: Anyone File name: NMR_data.zip Content type: Data Description: Nuclear magnetic spectroscopy data for PhotoMet synthesis (pdf) Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 222kB

HPLC data [74kB] HPLC data [74kB] HPLC data [74kB] Visible to: Anyone File name: HPLC_data.zip Content type: Data Description: Analytical High performance liquid chromatography data for tracer-FITC and probe_TAMRA (excel file) Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 74kB

SDS-PAGE data [10MB] SDS-PAGE data [10MB] SDS-PAGE data [10MB] Visible to: Anyone File name: SDS_PAGE_data.zip Content type: Data Description: SDS-polyacrylamide gel electrophoresis data for Figs.4a, 4c, S1, S3, S4, S5, S6, S7 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 10MB

flow cytometry data [117MB] flow cytometry data [117MB] flow cytometry data [117MB] Visible to: Anyone File name: FCS_data.zip Content type: Data Description: Flow cytometry data for Fig.2, 4 and S2 (as .fcs, .pdf files) Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 117MB

Microsopy data [1MB] Microsopy data [1MB] Microsopy data [1MB] Visible to: Anyone File name: Microscopy_data.zip Content type: Data Description: Fluorescence microscopy data for Fig. 3 as jpg files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB