Research Data Leeds Repository

Dataset associated with the "Effect of heparin and heparan sulphate on open promoter complex formation for a simple tandem gene model system using ex-situ atomic force microscopy"

Chammas, Oliver and Bonass, William A. and Thomson, Neil H. (2017) Dataset associated with the "Effect of heparin and heparan sulphate on open promoter complex formation for a simple tandem gene model system using ex-situ atomic force microscopy". University of Leeds. [Dataset] https://doi.org/10.5518/145

Dataset description

Dataset associated with the publication "Effect of heparin and heparan sulphate on open promoter complex formation for a simple tandem gene model system using ex-situ atomic force microscopy". The dataset includes the raw AFM images and statistical analysis. It is divided into six parts associated with the following AFM imaging data collected on: 1) cleaved mica; 2) polysaccharide heparin deposited on mica; 3) polysaccharide heparan sulphate deposited on mica; 4) the DNA template with and without the polysaccharides; 5) RNA polymerase with and without the polysaccharides; 6) Open promoter complexes with and without the polysaccharides.

Keywords:	Heparin, Heparan Sulphate, RNA polymerase, DNA, AFM, mica
Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C710 - Applied molecular biology, biophysics & biochemistry C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C760 - Biomolecular science C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C770 - Biophysical science F000 - Physical sciences > F300 - Physics > F310 - Applied physics
Divisions:	Faculty of Engineering and Physical Sciences > School of Physics and Astronomy Faculty of Medicine and Health > The Leeds Dental Institute
Related resources:	Location Type http://eprints.whiterose.ac.uk/115652/ Publication https://doi.org/10.1016/j.ymeth.2017.04.010 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	14 Sep 2017 15:56
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/247
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Chammas, Oliver Bonass, William A. https://orcid.org/0000-0003-4595-8266 Thomson, Neil H. https://orcid.org/0000-0001-7332-790X Type of data: Dataset Research funders: EPSRC Collection period: Date from Date to 1 November 2015 1 April 2016 Grant numbers: EP/J500124/1 Publication date: 2017 Resource language: English Metadata language: English Research project title: Concurrent DNA transcription from convergent and tandem promoters studied by atomic force microscopy Publisher: University of Leeds Contact Email Address: N.H.Thomson@leeds.ac.uk Last Modified: 14 Sep 2017 22:45

Files

Data

Bare mica controls - raw AFM images - Nanoscope [18MB] Bare mica controls - raw AFM images - Nanoscope [18MB] Bare mica controls - raw AFM images - Nanoscope [18MB] Visible to: Anyone File name: bare_mica_data_set.zip Content type: Data Description: Bare mica controls - raw AFM images - Nanoscope Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 18MB

Heparin on mica - Raw AFM images - Nanoscope [43MB] Heparin on mica - Raw AFM images - Nanoscope [43MB] Heparin on mica - Raw AFM images - Nanoscope [43MB] Visible to: Anyone File name: heparin_data_set.zip Content type: Data Description: Heparin on mica - Raw AFM images - Nanoscope Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 43MB

Heparan Sulphate on mica - Raw AFM images - Nanoscope [34MB] Heparan Sulphate on mica - Raw AFM images - Nanoscope [34MB] Heparan Sulphate on mica - Raw AFM images - Nanoscope [34MB] Visible to: Anyone File name: heparan_sulphate_data_set.zip Content type: Data Description: Heparan Sulphate on mica - Raw AFM images - Nanoscope Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 34MB

DNA on mica [262MB] DNA on mica [262MB] DNA on mica [262MB] Visible to: Anyone File name: dna_data_set.zip Content type: Data Description: DNA on mica, in the absence and presence of heparin or heparan sulphate. Raw AFM images - Nanoscope Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 262MB

RNA polymerase on mica [119MB] RNA polymerase on mica [119MB] RNA polymerase on mica [119MB] Visible to: Anyone File name: rnap_data_set.zip Content type: Data Description: RNA polymerase on mica in the absence and presence of heparin or heparan sulphate. Raw AFM images - Nanoscope. Counting Data for surface density Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 119MB

Open Promoter Complexes [173MB] Open Promoter Complexes [173MB] Open Promoter Complexes [173MB] Visible to: Anyone File name: open_promoter_complex_data_set.zip Content type: Data Description: Open Promoter Complexes in the absence and presence of heparin or heparan sulphate. Raw AFM images - Nanoscope. DNA contour length data. Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 173MB