Research Data Leeds Repository

Citation

Dewison, Katherine M., Chakraborty, Pijush, Brockwell, David J. and Radford, Sheena E. (2026) Data for 'The cassette exons of α-synuclein play distinct roles in membrane remodelling and membrane-mediated amyloid formation'. University of Leeds. [Dataset] https://doi.org/10.5518/1869

Dataset description

Dataset for 'The cassette exons of α-synuclein play distinct roles in membrane remodelling and membrane-mediated amyloid formation'. Containing raw data including circular dichroism, NMR, and thioflavin T fluorescence data.

Keywords:	amyloid; synuclein; protein; lipids
Divisions:	Faculty of Biological Sciences > School of Molecular and Cellular Biology
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	02 Nov 2026 10:41
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/1658
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Dewison, Katherine M. https://orcid.org/0000-0002-7196-7687 University of Leeds https://ror.org/024mrxd33 ror Chakraborty, Pijush https://orcid.org/0000-0003-1749-5225 University of Leeds https://ror.org/024mrxd33 ror Brockwell, David J. https://orcid.org/0000-0002-0802-5937 University of Leeds https://ror.org/024mrxd33 ror Radford, Sheena E. https://orcid.org/0000-0002-3079-8039 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: MRC, Wellcome, Royal Society Grant numbers: 094232/Z/10/Z, RSRP/R1/211057 Publication date: 2026 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: fbskdew@leeds.ac.uk Last Modified: 02 Nov 2026 10:41

Files

Documentation

readme_Dewison_ChemSci_2026.txt [2kB] readme_Dewison_ChemSci_2026.txt [2kB] readme_Dewison_ChemSci_2026.txt [2kB] Security: Anyone File name: readme_Dewison_ChemSci_2026.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2kB

Data

Far-UV CD spectra of aSyn variants at LPRs ranging from 0:1 to 100:1 ([M]:[M]) DMPS liposomes in 20 mM sodium phosphate buffer, pH 6.5, at 30 °C [58kB] Far-UV CD spectra of aSyn variants at LPRs ranging from 0:1 to 100:1 ([M]:[M]) DMPS liposomes in 20 mM sodium phosphate buffer, pH 6.5, at 30 °C [58kB] Far-UV CD spectra of aSyn variants at LPRs ranging from 0:1 to 100:1 ([M]:[M]) DMPS liposomes in 20 mM sodium phosphate buffer, pH 6.5, at 30 °C [58kB] Security: Anyone File name: Data_fig2.xlsx Content type: Data Description: Fig 2a-d - MRE for aSynFL, D3, D5, D3D5 at varying LPRs Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 58kB

NMR intensity ratios and CSPs [32kB] NMR intensity ratios and CSPs [32kB] NMR intensity ratios and CSPs [32kB] Security: Anyone File name: Data_fig3.xlsx Content type: Data Description: Fig 3 and SI Fig 2 - intensity ratios in the presence or absence of DMPS liposomes; chemical shift perturbations Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 32kB

Lipid-induced amyloid formation of the alternative splice variants of aSyn [70kB] Lipid-induced amyloid formation of the alternative splice variants of aSyn [70kB] Lipid-induced amyloid formation of the alternative splice variants of aSyn [70kB] Security: Anyone File name: Data_fig5.xlsx Content type: Data Description: Fig 5 - ThT for the splice variants incubated with DMPS at 30 degrees Celcius Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 70kB

Effect of a temperature jump on the self-assembly kinetics [166kB] Effect of a temperature jump on the self-assembly kinetics [166kB] Effect of a temperature jump on the self-assembly kinetics [166kB] Security: Anyone File name: Data_fig6.xlsx Content type: Data Description: Fig 6 - ThT for the splice variants incubated with DMPS with a temperature jump Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 166kB

Fluorescence polarisation of DPH-embedded DMPS liposomes [21kB] Fluorescence polarisation of DPH-embedded DMPS liposomes [21kB] Fluorescence polarisation of DPH-embedded DMPS liposomes [21kB] Security: Anyone File name: Data_SI_fig3.xlsx Content type: Data Description: SI fig 3 - fluorescence polarisation of DMPS liposomes in the prescence of the different splice variants Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 21kB

DLPS-induced self-assembly of the alternative splice variants of αSyn [69kB] DLPS-induced self-assembly of the alternative splice variants of αSyn [69kB] DLPS-induced self-assembly of the alternative splice variants of αSyn [69kB] Security: Anyone File name: Data_SI_fig6.xlsx Content type: Data Description: SI fig 6 - ThT for the splice variants incubated with DLPS at 30 degrees Celcius Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 69kB