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Dataset associated with 'Biomimetic curvature and tension-driven membrane fusion by silica nanoparticles’
Citation
Arribas Perez, Marcos (2021) Dataset associated with 'Biomimetic curvature and tension-driven membrane fusion by silica nanoparticles’. University of Leeds. [Dataset] https://doi.org/10.5518/929
Dataset description
Experimental data obtained from FRET spectroscopy and confocal microscopy imaging of lipid vesicles interacting with silica nanoparticles. Experimental set up and data processing and analysis described in the publication in Langmuir. Confocal microscopy movies of giant unilamellar vesicles undergoing fusion upon interaction with silica nanoparticles.
| Subjects: | C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry | ||||||
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| Divisions: | Faculty of Engineering and Physical Sciences > School of Chemistry | ||||||
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| License: | Creative Commons Attribution 4.0 International (CC BY 4.0) | ||||||
| Date deposited: | 29 Nov 2021 16:48 | ||||||
| URI: | https://archive.researchdata.leeds.ac.uk/id/eprint/905 | ||||||
Files
Data
FRET data analysis (Figure 1) [45kB]  |
Direct fusion fluorescence analysis (Figure 4) [328kB] |
Hemifusion-fusion fluorescence analysis (Figure 5) [392kB] |
Gentle merging fluorescence analysis (Figure 6) [453kB] |
Lipid mixing calculations (Figure 7) [345kB] |
Lipid mixing data analysis and plots (Figure 7) [3MB] |
Supplementary Movie 1 (Figure 2a) [637MB] |
Supplementary Movie 2 (Figure 2b) [69MB] |
Supplementary Movie 3 (Figure 2c) [4MB] |
Supplementary Movie 4 (Figure 4) [2MB] |
Supplementary Movie 4 no zoom (Figure 4) [61MB] |
Supplementary Movie 5 (Figure 5) [1GB] |
Supplementary Movie 6 (Figure 6) [255MB] |
Laurdan GP from GUVs images (Figure 3a) [243kB] |
Size distribution of silica NPs from TEM images (Figure S1) [72kB] |
Calculation of volume ratio of initial and final GUVs (Figure S5) [17kB] |
Volume ratio of initial and final GUVs data analysis and plots (Figure S5) [123kB] |
Influence of membrane tension in fusion efficiency: Quantification of lipid mixed GUVs per tile scan (Figure 3b) [12kB] |
Influence of membrane tension in fusion efficiency: Plots of lipid mixed GUVs per tile scan (Figure 3b) [606kB] |
FRET data analysis (Figure 1) [45kB] 
FRET data analysis (Figure 1) [45kB]