Research Data Leeds Repository

Citation

Ingham, Eileen and Jones, Gemma and Herbert, Anthony and Berry, Helen and Edwards, Jennifer and Fisher, John (2016) Decellularisation and characterisation of porcine superflexor tendon: a potential anterior cruciate ligament replacement. University of Leeds. [Dataset] https://doi.org/10.5518/39

Dataset description

Data on the biological and biomechanical properties of porcine superflexor tendon grafts before and after decellularisation: biomechanical testing data (ultimate tensile strength, failure strain, Young's modulus); DNA, collagen and GAG content; semi-quantitative alpha Gal analysis; in vitro and in vivo biocompatibility.

Keywords:	Anterior cruciate ligament, knee reconstruction, porcine, decellularisation, acellular graft
Subjects:	A000 - Medicine & dentistry > A100 - Pre-clinical medicine H000 - Engineering > H100 - General engineering > H160 - Bioengineering, biomedical engineering & clinical engineering > H161 - Biomaterials H000 - Engineering > H100 - General engineering > H160 - Bioengineering, biomedical engineering & clinical engineering > H162 - Biomechanics (including fluid & solid mechanics) H000 - Engineering > H100 - General engineering > H160 - Bioengineering, biomedical engineering & clinical engineering > H165 - Tissue engineering & regenerative medicine H000 - Engineering > H100 - General engineering > H160 - Bioengineering, biomedical engineering & clinical engineering > H168 - Medical devices & instrumentation B000 - Subjects allied to medicine > B800 - Medical technology C000 - Biological sciences > C900 - Others in Biological Sciences > C910 - Applied biological sciences
Divisions:	Faculty of Biological Sciences > Institute of Medical and Biological Engineering Faculty of Engineering and Physical Sciences > Institute of Medical and Biological Engineering Faculty of Engineering and Physical Sciences > School of Mechanical Engineering > Institute of Medical and Biological Engineering
Related resources:	Location Type https://doi.org/10.1089/ten.TEA.2016.0114 Publication http://eprints.whiterose.ac.uk/106269/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	25 Oct 2016 15:42
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/89
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Ingham, Eileen https://orcid.org/0000-0002-9757-3045 Jones, Gemma https://orcid.org/0000-0002-7022-921X Herbert, Anthony https://orcid.org/0000-0002-6527-2205 Berry, Helen https://orcid.org/0000-0003-2426-1881 Edwards, Jennifer https://orcid.org/0000-0003-4152-6140 Fisher, John https://orcid.org/0000-0003-3833-3700 Type of data: Dataset Research funders: Wellcome Trust, EPSRC, ERC Grant numbers: WT088908/z/09/z, 267114REGENKNEE Publication date: 2016 Resource language: English Metadata language: English Research project title: Engineering solutions for an ageing population with musculoskeletal & cardiovascular disease. 50 more years after 50., Re engineering and regenerating the knee, (regenknee) Publisher: University of Leeds Copyright holders: University of Leeds Contact email: E.ingham@leeds.ac.uk Last Modified: 22 Jun 2017 10:11

Files

Documentation

Readme [710B] Readme [710B] Readme [710B] Security: Anyone File name: Readme.txt Content type: Documentation Description: Description of dataset Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 710B

Data

Raw data and analysis [88kB] Raw data and analysis [88kB] Raw data and analysis [88kB] Security: Anyone File name: Raw_Data_and_Statistical_Analysis.xlsx Content type: Data Description: Details of tissue weights, solution volumes and analysis for the DNA assay, hydroxyproline assay, GAG assay, extract cytotoxicity data, semiquantitative alph-Gal assay. Details of biomechanical test results and analysis Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 88kB

Gross images of in vivo biocompatibility [621kB] Gross images of in vivo biocompatibility [621kB] Gross images of in vivo biocompatibility [621kB] Security: Anyone File name: Gross_images_of_tissues_explanted_from_mice.docx Content type: Data Description: Mouse numbers and images of the explanted native and decellularised sft in situ Mime-Type: application/vnd.openxmlformats-officedocument.wordprocessingml.document License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 621kB

Histology images of in vivo biocompatibility [27MB] Histology images of in vivo biocompatibility [27MB] Histology images of in vivo biocompatibility [27MB] Security: Anyone File name: Histology_images_of_tissues_explanted_from_mice.zip Content type: Data Description: Images of H&E stained sections of explanted native and decellularised tissues Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 27MB