Research Data Leeds Repository

Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids

Wehrum, Sarah and Siukstaite, Lina and Williamson, Daniel J. and Branson, Thomas R. and Sych, Taras and Madl, Josef and Wildsmith, Gemma C. and Dai, Wenyue and Kempmann, Erik and Ross, James F. and Thomsen, Maren and Webb, Michael E. and Römer, Winfried and Turnbull, W. Bruce (2022) Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids. University of Leeds. [Dataset] https://doi.org/10.5518/1250

Subjects:	F000 - Physical sciences > F100 - Chemistry > F160 - Organic chemistry > F165 - Biomolecular chemistry F000 - Physical sciences > F100 - Chemistry > F160 - Organic chemistry C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C720 - Biological chemistry
Divisions:	Faculty of Engineering and Physical Sciences > School of Chemistry
Related resources:	Location Type https://doi.org/10.1021/acssynbio.2c00266 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	17 Nov 2022 11:50
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/1046
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Wehrum, Sarah https://orcid.org/0000-0002-9671-036X University of Freiburg https://ror.org/0245cg223 ror Siukstaite, Lina https://orcid.org/0000-0002-0899-1916 University of Freiburg https://ror.org/0245cg223 ror Williamson, Daniel J. University of Leeds https://ror.org/024mrxd33 ror Branson, Thomas R. University of Leeds https://ror.org/024mrxd33 ror Sych, Taras https://orcid.org/0000-0002-7330-9063 University of Freiburg https://ror.org/0245cg223 ror Madl, Josef University of Freiburg https://ror.org/0245cg223 ror Wildsmith, Gemma C. University of Leeds https://ror.org/024mrxd33 ror Dai, Wenyue https://orcid.org/0000-0003-3904-2227 University of Leeds https://ror.org/024mrxd33 ror Kempmann, Erik University of Freiburg https://ror.org/0245cg223 ror Ross, James F. https://orcid.org/0000-0002-4784-103X University of Leeds https://ror.org/024mrxd33 ror Thomsen, Maren https://orcid.org/0000-0003-2561-7595 University of Leeds https://ror.org/024mrxd33 ror Webb, Michael E. https://orcid.org/0000-0003-3574-4686 University of Leeds https://ror.org/024mrxd33 ror Römer, Winfried https://orcid.org/0000-0002-2847-246X University of Freiburg https://ror.org/0245cg223 ror Turnbull, W. Bruce https://orcid.org/0000-0002-7352-0360 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: BBSRC, EPSRC, Wellcome Trust, European Union, German Federal Ministry of Education and Research, Ministry of Science, Research and the Arts of Baden-Württemberg, German Research Foundation Grant numbers: BB/M011151/1, BB/L015056/1, EP/G043302/1, 089308/Z/09/Z, 094232/Z/10/Z, 708051, 814029, FKZ 031A464, Az: 33-7532.20, EXC 294 Publication date: 2022 Resource language: English Publisher: University of Leeds Contact Email Address: w.b.turnbull@leeds.ac.uk Last Modified: 02 Feb 2024 09:29

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Data

ESMS data [1MB] ESMS data [1MB] ESMS data [1MB] Visible to: Anyone File name: ESMS_data.zip Content type: Data Description: Electrospray mass spectrometry data for Figs. S8, S10, S14 as PDF files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

Fluorescence data Fig. 3 [271MB] Fluorescence data Fig. 3 [271MB] Fluorescence data Fig. 3 [271MB] Visible to: Anyone File name: Fluorescence_data_Fig.3.zip Content type: Data Description: Fluroescence microscopy data for Fig. 3 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 271MB

Fluorescence data Fig. 6 [273MB] Fluorescence data Fig. 6 [273MB] Fluorescence data Fig. 6 [273MB] Visible to: Anyone File name: Fluorescence_data_Fig.6.zip Content type: Data Description: Fluroescence microscopy data for Fig. 6 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 273MB

Fluorescence data Fig. S2 [24MB] Fluorescence data Fig. S2 [24MB] Fluorescence data Fig. S2 [24MB] Visible to: Anyone File name: Fluorescence_data_Fig.S2.zip Content type: Data Description: Fluroescence microscopy data for Fig. S2 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 24MB

Fluorescence data Fig. S4 [8MB] Fluorescence data Fig. S4 [8MB] Fluorescence data Fig. S4 [8MB] Visible to: Anyone File name: Fluorescence_data_Fig.S4.zip Content type: Data Description: Fluroescence microscopy data for Fig. S4 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8MB

Fluorescence data Figs. S5 S6 [1GB] Fluorescence data Figs. S5 S6 [1GB] Fluorescence data Figs. S5 S6 [1GB] Visible to: Anyone File name: Fluorescence_data_Figs.S5S6.zip Content type: Data Description: Fluroescence microscopy data for Fig. S5 and S6 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1GB

Fluorescence data Lo membranes [6MB] Fluorescence data Lo membranes [6MB] Fluorescence data Lo membranes [6MB] Visible to: Anyone File name: Fluorescence_data_Lo_membranes.zip Content type: Data Description: Fluorescence microscopy data additional examples of Lo membranes as mp4 files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 6MB

Fluorescence data negative control [33MB] Fluorescence data negative control [33MB] Fluorescence data negative control [33MB] Visible to: Anyone File name: Fluorescence_data_Negative_control_GM1_GUV_without_StrepAB5n_protein.zip Content type: Data Description: Fluorescence microscopy data Negative_control_GM1_GUV_without_Strep-(AB5)n_protein as .avi file Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 33MB

ITC data [18kB] ITC data [18kB] ITC data [18kB] Visible to: Anyone File name: ITC_data.zip Content type: Data Description: Isothermal titration calorimetry data for Fig. 2 as .itc text files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 18kB

NMR data [11MB] NMR data [11MB] NMR data [11MB] Visible to: Anyone File name: NMR_data.zip Content type: Data Description: Nuclear magnetic spectroscopy fid data for Figs. S12 and S13 (Bruker format files) Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 11MB

SDS-PAGE data [1MB] SDS-PAGE data [1MB] SDS-PAGE data [1MB] Visible to: Anyone File name: SDS_PAGE_data.zip Content type: Data Description: SDS-polyacrylamide gel electrophoresis data for Figs. 2 and S9 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

SEC-MALLS data [2MB] SEC-MALLS data [2MB] SEC-MALLS data [2MB] Visible to: Anyone File name: SEC_MALLS_data.zip Content type: Data Description: Size exclusion chromatography- multi angle light scattering data for Fig 2 (png, pdf, csv, xlsx, afe6 files) Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2MB