1. ABOUT THE DATASET Title: Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids Creators: Sarah Wehrum, Lina Siukstaite, Daniel J Williamson, Thomas R Branson, Taras Sych, Josef Madl, Gemma C Wildsmith, Wenyue Dai, Erik Kempmann, James F Ross, Maren Thomsen, Michael E Webb, Winfried Römer, W Bruce Turnbull Organisation: University of Leeds, University of Freiburg Publication Year: 2022 Description: Data for characterising the identity, purity and membrane-modifying properties of protein complexes derived from non-toxic subunits of cholera toxin and streptavidin, including: fluorescence microscopy images of giant unilamellar vesicle experiments; isothermal titration calorimetry of complex formation; 1H and 13C NMR spectra for biotin derivatives and mass spectrometry data, SEC-MALLS analysis, and SDS-polyacrylamide gel electrophoresis for modified proteins. Cite as: Sarah Wehrum, Lina Siukstaite, Daniel J Williamson, Thomas R Branson, Taras Sych, Josef Madl, Gemma C Wildsmith, Wenyue Dai, Erik Kempmann, James F Ross, Maren Thomsen, Michael E Webb, Winfried Römer, W Bruce Turnbull (2022): Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids. University of Leeds. [Dataset] https://doi.org/10.5518/1250 Related publication: Sarah Wehrum, Lina Siukstaite, Daniel J Williamson, Thomas R Branson, Taras Sych, Josef Madl, Gemma C Wildsmith, Wenyue Dai, Erik Kempmann, James F Ross, Maren Thomsen, Michael E Webb, Winfried Römer, W Bruce Turnbull, Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids. ACS Synthetic Biology. https://doi=10.1021/acssynbio.2c00266 Contact: w.b.turnbull@leeds.ac.uk 2. TERMS OF USE This dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/.] 3. PROJECT AND FUNDING INFORMATION Funding organisation: BBSRC; EPSRC; Wellcome Trust; European Union; German Federal Ministry of Education and Research; Ministry of Science, Research and the Arts of Baden-Württemberg; German Research Foundation; Franco-German University (programs ‘Polymer Sciences’ and ‘Cotutelle de these’); the College Doctoral Europeen (PDI); the International Graduate Academy (University of Freiburg) Grant no.: EPSRC: EP/G043302/1 BBSRC: BB/M011151/1; BB/L015056/1 Wellcome: 089308/Z/09/Z; 094232/Z/10/Z European Union (Horizon 2020): 708051; 814029 German Federal Ministry of Education and Research (BMBF): FKZ 031A464 Ministry of Science, Research and the Arts of Baden-Württemberg: Az: 33-7532.20   4. CONTENTS ESMS data.zip PDF files of electrospray mass spectrometry data used to construct: Fig S8 (A2/CTB AB5 protein derived from pTRBAB5-G1S) Fig S10 (A2 peptide following oxime ligation with Biotin-1) Fig S14 (A2 peptide following oxime ligation with Biotin-2) Fluorescence data Fig. 3.zip Confocal microscopy time course data as .tif file Fluorescence data Fig. 6.zip Confocal microscopy time course data as .tif files Fluorescence data Fig. S2.zip Confocal microscopy time course data as .tif files Fluorescence data Fig. S4.zip Confocal microscopy data as .tif files Fluorescence data Figs. S5 S6.zip Confocal microscopy time course data as .tif files Fluorescence data Lo membranes.zip Confocal microscopy time course data as .mp4 files Fluorescence data Negative_control_GM1_GUV_without_Strep-(AB5)n_protein.zip Confocal microscopy time course data as .avi file ITC data.zip MicroCal isothermal titration calorimetry data files for streptavidin – biotin-AB5 titration and biotin-AB5 dilution into buffer as .itc text files NMR data.zip Bruker FID data and associated files for the 1H, 13C, COSY and HSQC spectra where available for compounds shown below. N-(3-(N-Boc-aminooxy)propyl)phthalimide 5 Boc-protected biotin-SS-oxyamine 7 SDS-PAGE data.zip SDS-polyacrylamide gel electrophoresis data for Figs. 2 and S9 as .tif files SEC-MALLS data.zip Size exclusion chromatography- multi angle light scattering data for Fig 2 (.png, .pdf, .csv, .xlsx, .afe6 files) 5. METHODS Detailed information about methods is available in the related publication in Section 1 above.