Research Data Leeds Repository

Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids

Citation

Wehrum, Sarah and Siukstaite, Lina and Williamson, Daniel J. and Branson, Thomas R. and Sych, Taras and Madl, Josef and Wildsmith, Gemma C. and Dai, Wenyue and Kempmann, Erik and Ross, James and Thomsen, Maren and Webb, Michael E. and Römer, Winfried and Turnbull, W. Bruce (2022) Membrane fusion mediated by non-covalent binding of re-engineered cholera toxin assemblies to glycolipids. University of Leeds. [Dataset] https://doi.org/10.5518/1250¹

Subjects:	F000 - Physical sciences > F100 - Chemistry > F160 - Organic chemistry > F165 - Biomolecular chemistry2 F000 - Physical sciences > F100 - Chemistry > F160 - Organic chemistry3 C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry4 C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C720 - Biological chemistry5
Divisions:	Faculty of Engineering and Physical Sciences > School of Chemistry6
Related resources:	Location Type https://doi.org/10.1021/acssynbio.2c002667 Publication https://eprints.whiterose.ac.uk/193337/8 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	17 Nov 2022 11:50
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/10469
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Wehrum, Sarah https://orcid.org/0000-0002-9671-036X10 University of Freiburg https://ror.org/0245cg223 ror Siukstaite, Lina https://orcid.org/0000-0002-0899-191611 University of Freiburg https://ror.org/0245cg223 ror Williamson, Daniel J. University of Leeds https://ror.org/024mrxd33 ror Branson, Thomas R. University of Leeds https://ror.org/024mrxd33 ror Sych, Taras https://orcid.org/0000-0002-7330-906312 University of Freiburg https://ror.org/0245cg223 ror Madl, Josef University of Freiburg https://ror.org/0245cg223 ror Wildsmith, Gemma C. University of Leeds https://ror.org/024mrxd33 ror Dai, Wenyue https://orcid.org/0000-0003-3904-222713 University of Leeds https://ror.org/024mrxd33 ror Kempmann, Erik University of Freiburg https://ror.org/0245cg223 ror Ross, James https://orcid.org/0000-0002-4784-103X14 University of Leeds https://ror.org/024mrxd33 ror Thomsen, Maren https://orcid.org/0000-0003-2561-759515 University of Leeds https://ror.org/024mrxd33 ror Webb, Michael E. https://orcid.org/0000-0003-3574-468616 University of Leeds https://ror.org/024mrxd33 ror Römer, Winfried https://orcid.org/0000-0002-2847-246X17 University of Freiburg https://ror.org/0245cg223 ror Turnbull, W. Bruce https://orcid.org/0000-0002-7352-036018 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: BBSRC, EPSRC, Wellcome Trust, European Union, German Federal Ministry of Education and Research, Ministry of Science, Research and the Arts of Baden-Württemberg, German Research Foundation Grant numbers: BB/M011151/1, BB/L015056/1, EP/G043302/1, 089308/Z/09/Z, 094232/Z/10/Z, 708051, 814029, FKZ 031A464, Az: 33-7532.20, EXC 294 Publication date: 2022 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: w.b.turnbull@leeds.ac.uk19 Last Modified: 03 Sep 2024 11:43

Files

Documentation

readme [4kB] 20 readme [4kB] 21 readme [4kB] 22 Security: Anyone File name: readme_Wehrum_etal_2022.txt23 Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 4kB

Data

ESMS data [1MB] 24 ESMS data [1MB] 25 ESMS data [1MB] 26 Security: Anyone File name: ESMS_data.zip27 Content type: Data Description: Electrospray mass spectrometry data for Figs. S8, S10, S14 as PDF files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

Fluorescence data Fig. 3 [271MB] 28 Fluorescence data Fig. 3 [271MB] 29 Fluorescence data Fig. 3 [271MB] 30 Security: Anyone File name: Fluorescence_data_Fig.3.zip31 Content type: Data Description: Fluroescence microscopy data for Fig. 3 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 271MB

Fluorescence data Fig. 6 [273MB] 32 Fluorescence data Fig. 6 [273MB] 33 Fluorescence data Fig. 6 [273MB] 34 Security: Anyone File name: Fluorescence_data_Fig.6.zip35 Content type: Data Description: Fluroescence microscopy data for Fig. 6 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 273MB

Fluorescence data Fig. S2 [24MB] 36 Fluorescence data Fig. S2 [24MB] 37 Fluorescence data Fig. S2 [24MB] 38 Security: Anyone File name: Fluorescence_data_Fig.S2.zip39 Content type: Data Description: Fluroescence microscopy data for Fig. S2 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 24MB

Fluorescence data Fig. S4 [8MB] 40 Fluorescence data Fig. S4 [8MB] 41 Fluorescence data Fig. S4 [8MB] 42 Security: Anyone File name: Fluorescence_data_Fig.S4.zip43 Content type: Data Description: Fluroescence microscopy data for Fig. S4 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8MB

Fluorescence data Figs. S5 S6 [1GB] 44 Fluorescence data Figs. S5 S6 [1GB] 45 Fluorescence data Figs. S5 S6 [1GB] 46 Security: Anyone File name: Fluorescence_data_Figs.S5S6.zip47 Content type: Data Description: Fluroescence microscopy data for Fig. S5 and S6 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1GB

Fluorescence data Lo membranes [6MB] 48 Fluorescence data Lo membranes [6MB] 49 Fluorescence data Lo membranes [6MB] 50 Security: Anyone File name: Fluorescence_data_Lo_membranes.zip51 Content type: Data Description: Fluorescence microscopy data additional examples of Lo membranes as mp4 files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 6MB

Fluorescence data negative control [33MB] 52 Fluorescence data negative control [33MB] 53 Fluorescence data negative control [33MB] 54 Security: Anyone File name: Fluorescence_data_Negative_control_GM1_GUV_without_StrepAB5n_protein.zip55 Content type: Data Description: Fluorescence microscopy data Negative_control_GM1_GUV_without_Strep-(AB5)n_protein as .avi file Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 33MB

ITC data [18kB] 56 ITC data [18kB] 57 ITC data [18kB] 58 Security: Anyone File name: ITC_data.zip59 Content type: Data Description: Isothermal titration calorimetry data for Fig. 2 as .itc text files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 18kB

NMR data [11MB] 60 NMR data [11MB] 61 NMR data [11MB] 62 Security: Anyone File name: NMR_data.zip63 Content type: Data Description: Nuclear magnetic spectroscopy fid data for Figs. S12 and S13 (Bruker format files) Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 11MB

SDS-PAGE data [1MB] 64 SDS-PAGE data [1MB] 65 SDS-PAGE data [1MB] 66 Security: Anyone File name: SDS_PAGE_data.zip67 Content type: Data Description: SDS-polyacrylamide gel electrophoresis data for Figs. 2 and S9 as tif files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

SEC-MALLS data [2MB] 68 SEC-MALLS data [2MB] 69 SEC-MALLS data [2MB] 70 Security: Anyone File name: SEC_MALLS_data.zip71 Content type: Data Description: Size exclusion chromatography- multi angle light scattering data for Fig 2 (png, pdf, csv, xlsx, afe6 files) Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2MB

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