Research Data Leeds Repository

Citation

Basu, Basudha, Lake, Alice, Szymanska, Katarzyna, Wheway, Gabrielle, Bell, Sandra, Morrison, Ewan, Bond, Jacqueline and Johnson, Colin A. (2021) Reverse genetics siRNA-based visual phenotype screen datasets identifying genes involved in supernumerary primary cilium formation. University of Leeds. [Dataset] https://doi.org/10.5518/1044

Dataset description

High content siRNA screen carried out to identify genes which when knocked-down, cause the formation of multiple primary cilia in the mIMCD3 cell-line. The dataset includes data from both the primary and secondary screens as separate files.

Keywords:	Cilia, high-content imaging
Subjects:	C000 - Biological sciences > C100 - Biology > C130 - Cell biology
Divisions:	Faculty of Medicine and Health > School of Medicine
Related resources:	Location Type https://doi.org/10.1111/ahg.12529 Publication https://eprints.whiterose.ac.uk/205224/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	18 Oct 2021 10:27
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/898
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Basu, Basudha https://orcid.org/0000-0002-5434-5202 University of Leeds https://ror.org/024mrxd33 ror Lake, Alice https://orcid.org/0000-0002-6341-8661 University of Leeds https://ror.org/024mrxd33 ror Massey University https://ror.org/052czxv31 ror Szymanska, Katarzyna https://orcid.org/0000-0001-7736-5225 University of Leeds https://ror.org/024mrxd33 ror Wheway, Gabrielle https://orcid.org/0000-0002-0494-0783 University Hospital Southampton NHS Foundation Trust https://ror.org/0485axj58 ror University of Southampton https://ror.org/01ryk1543 ror Bell, Sandra https://orcid.org/0000-0002-3022-9864 University of Leeds https://ror.org/024mrxd33 ror Morrison, Ewan https://orcid.org/0000-0001-6269-6638 University of Leeds https://ror.org/024mrxd33 ror Bond, Jacqueline https://orcid.org/0000-0001-5390-5688 University of Leeds https://ror.org/024mrxd33 ror Johnson, Colin A. https://orcid.org/0000-0002-2979-8234 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: MRC, BBSRC-SFI, European Community's Seventh Framework Programme FP7/2009 Grant numbers: MR/M000532/1, BB/P007791/1, 241955 SYSCILIA Publication date: 2021 Resource language: English Metadata language: English Research project title: Functional genomics identification and characterization of novel disease egenes, mechanisms and pathways of ciliogenesis, Structure-function relationships in the ciliary transition zone, A systems biology approach to dissect cilia function and its disruption in human genetic disease Publisher: University of Leeds Contact email: c.johnson@leeds.ac.uk Last Modified: 28 Jun 2024 14:54

Files

Data

Primary screen data [1MB] Primary screen data [1MB] Primary screen data [1MB] Security: Anyone File name: Primary_ciliascreen.txt Content type: Data Mime-Type: text/plain File size: 1MB

Secondary screen data [10kB] Secondary screen data [10kB] Secondary screen data [10kB] Security: Anyone File name: Secondary_ciliascreen.txt Content type: Data Mime-Type: text/plain File size: 10kB