Research Data Leeds Repository

De Novo Designed Peptide and Protein Hairpins Self-assemble into Sheets and Nanoparticles - dataset

Citation

Galloway, Johanna M. and Bray, Harriet E. V. and Shoemark, Deborah K. and Hodgson, Lorna R. and Coombs, Jennifer and Mantell, Judith M. and Rose, Ruth S. and Ross, James and Morris, Caroline and Harniman, Robert L. and Wood, Christopher and Arthur, Christopher and Verkade, Paul and Woolfson, Derek N. (2021) De Novo Designed Peptide and Protein Hairpins Self-assemble into Sheets and Nanoparticles - dataset. University of Leeds. [Dataset] https://doi.org/10.5518/955¹

Dataset description

AFM, TEM, fluorescence microscopy image files and spectral data published in DOI:10.1002/smll.202100472. Preprint of this is available at bioRxiv DOI:10.1101/2020.08.14.251462

Keywords:	coiled coil, computational modeling, peptide design, protein design, self-assembly
Divisions:	Faculty of Engineering and Physical Sciences > School of Chemistry2
Related resources:	Location Type https://doi.org/10.1002/smll.2021004723 Publication https://doi.org/10.1101/2020.08.14.2514624 Publication https://eprints.whiterose.ac.uk/171326/5 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	03 Feb 2021 09:53
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/8116
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Galloway, Johanna M. https://orcid.org/0000-0003-3998-08707 University of Leeds https://ror.org/024mrxd33 ror University of Bristol https://ror.org/0524sp257 ror Bray, Harriet E. V. University of Bristol https://ror.org/0524sp257 Shoemark, Deborah K. https://orcid.org/0000-0002-1240-84638 University of Bristol https://ror.org/0524sp257 Hodgson, Lorna R. https://orcid.org/0000-0001-6373-44059 University of Bristol https://ror.org/0524sp257 Coombs, Jennifer University of Bristol https://ror.org/0524sp257 Mantell, Judith M. https://orcid.org/0000-0002-9061-893310 University of Bristol https://ror.org/0524sp257 Rose, Ruth S. https://orcid.org/0000-0001-6406-137X11 Queen Mary University of London https://ror.org/026zzn846 Ross, James https://orcid.org/0000-0002-4784-103X12 University of Bristol https://ror.org/0524sp257 Morris, Caroline https://orcid.org/0000-0002-4797-031713 University of Bristol https://ror.org/0524sp257 Harniman, Robert L. https://orcid.org/0000-0002-3452-121314 University of Bristol https://ror.org/0524sp257 Wood, Christopher https://orcid.org/0000-0003-1243-310515 University of Bristol https://ror.org/0524sp257 Arthur, Christopher https://orcid.org/0000-0001-7577-847216 University of Bristol https://ror.org/0524sp257 Verkade, Paul https://orcid.org/0000-0002-2497-102617 University of Bristol https://ror.org/0524sp257 Woolfson, Derek N. https://orcid.org/0000-0002-0394-320218 University of Bristol https://ror.org/0524sp257 Type of data: Dataset Research funders: BBSRC, EPSRC Grant numbers: BB/M002969/1, BB/L014181/1, BB/L01386X/1, EP/K035746/1, EP/K03927X/1 Publication date: 2021 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: j.m.galloway@leeds.ac.uk19 Last Modified: 03 Sep 2024 13:24

Files

Documentation

File listing for all zip files [6kB] 20 File listing for all zip files [6kB] 21 File listing for all zip files [6kB] 22 Security: Anyone File name: zip_file_manifest.txt23 Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 6kB

Data

F2_TEM_peptide [135MB] 24 F2_TEM_peptide [135MB] 25 F2_TEM_peptide [135MB] 26 Security: Anyone File name: F2_TEM_peptide.zip27 Content type: Data Description: TEM images and FFTs shown in Figure 2 separated files a to p Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 135MB

F3_AFM_peptide [316MB] 28 F3_AFM_peptide [316MB] 29 F3_AFM_peptide [316MB] 30 Security: Anyone File name: F3_AFM_peptide.zip31 Content type: Data Description: AFM plots, processing and processed data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 316MB

F4_TEM_protein [52MB] 32 F4_TEM_protein [52MB] 33 F4_TEM_protein [52MB] 34 Security: Anyone File name: F4_TEM_protein.zip35 Content type: Data Description: TEM images and FFTs shown in Figure 3 separated files a to j Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 52MB

S1_curvature_model [11kB] 36 S1_curvature_model [11kB] 37 S1_curvature_model [11kB] 38 Security: Anyone File name: S1_curvature.xlsx39 Content type: Data Description: data plotted in fig S1 Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 11kB

S2_nanospray_peptide [9MB] 40 S2_nanospray_peptide [9MB] 41 S2_nanospray_peptide [9MB] 42 Security: Anyone File name: S2_peptide_nanospray_data.xlsx43 Content type: Data Description: spectra plotted in fig S2 Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 9MB

S3_HPLC_peptide [76kB] 44 S3_HPLC_peptide [76kB] 45 S3_HPLC_peptide [76kB] 46 Security: Anyone File name: S3_HPLC_data.xlsx47 Content type: Data Description: absorbance plotted in fig S3 Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 76kB

S4_AFM_sheet_1 [1MB] 48 S4_AFM_sheet_1 [1MB] 49 S4_AFM_sheet_1 [1MB] 50 Security: Anyone File name: S4_AFM_sheet_1.zip51 Content type: Data Description: AFM plotted in fig S4 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

S5_AFM_sheet_2 [9MB] 52 S5_AFM_sheet_2 [9MB] 53 S5_AFM_sheet_2 [9MB] 54 Security: Anyone File name: S5_AFM_sheet_2.zip55 Content type: Data Description: AFM plotted in fig S5 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 9MB

S6_peptide_CLEM [110MB] 56 S6_peptide_CLEM [110MB] 57 S6_peptide_CLEM [110MB] 58 Security: Anyone File name: S6_peptide_CLEM.zip59 Content type: Data Description: fluorescence and TEM plotted in fig S6 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 110MB

S8_PAGE_nanospray_protein [6MB] 60 S8_PAGE_nanospray_protein [6MB] 61 S8_PAGE_nanospray_protein [6MB] 62 Security: Anyone File name: S8_PAGE_nanospray_protein.zip63 Content type: Data Description: spectra and SDS-PAGE plotted in figure S8 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 6MB

S9_pro_HP-DT_dist_cells_CLEM [85MB] 64 S9_pro_HP-DT_dist_cells_CLEM [85MB] 65 S9_pro_HP-DT_dist_cells_CLEM [85MB] 66 Security: Anyone File name: S9_pro_HP-DT_dist_cells_CLEM.zip67 Content type: Data Description: fluorescence and TEM plotted in fig S9 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 85MB

S10_pro_HP-DT_pH [235MB] 68 S10_pro_HP-DT_pH [235MB] 69 S10_pro_HP-DT_pH [235MB] 70 Security: Anyone File name: S10_pro_HP-DT_pH.zip71 Content type: Data Description: TEM images and FFTs shown in fig S10 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 235MB

S11_proGFP_HP-DT_dist_cells_CLEM [103MB] 72 S11_proGFP_HP-DT_dist_cells_CLEM [103MB] 73 S11_proGFP_HP-DT_dist_cells_CLEM [103MB] 74 Security: Anyone File name: S11_proGFP_HP-DT_dist_cells_CLEM.zip75 Content type: Data Description: fluorescence and TEM plotted in fig S11 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 103MB

S12_proGFP_HP-DT_dist_vitro_CLEM [66MB] 76 S12_proGFP_HP-DT_dist_vitro_CLEM [66MB] 77 S12_proGFP_HP-DT_dist_vitro_CLEM [66MB] 78 Security: Anyone File name: S12_proGFP_HP-DT_dist_vitro_CLEM.zip79 Content type: Data Description: fluorescence and TEM plotted in fig S12 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 66MB

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