Research Data Leeds Repository

Data for 'Kinetic Steering of Amyloid Formation'

Citation

Taylor, Alexander I. P. and Xu, Yong and Wilkinson, Martin and Chakraborty, Pijush and Brinkworth, Alice and Willis, Leon and Zhuravleva, Anastasia and Ranson, Neil A. and Foster, Richard and Radford, Sheena E. (2025) Data for 'Kinetic Steering of Amyloid Formation'. University of Leeds. [Dataset] https://doi.org/10.5518/1604¹

Dataset description

Data for the figures in "Kinetic Steering of Amyloid Formation by Canagliflozin, a Type-2 Diabetes Drug" by Taylor et al. (JACS, 2025).

Subjects:	C000 - Biological sciences2 F000 - Physical sciences > F100 - Chemistry3
Divisions:	Faculty of Biological Sciences > Astbury Centre for Structural Molecular Biology4 Faculty of Biological Sciences > School of Molecular and Cellular Biology5 Faculty of Engineering and Physical Sciences > School of Chemistry6
Related resources:	Location Type https://doi.org/10.1021/jacs.4c167437 Publication https://eprints.whiterose.ac.uk/223329/8 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	03 Mar 2025 16:26
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/13809
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Taylor, Alexander I. P. https://orcid.org/0000-0003-3131-361910 University of Leeds https://ror.org/024mrxd33 ror Xu, Yong https://orcid.org/0000-0002-8389-412011 University of Leeds https://ror.org/024mrxd33 ror Wilkinson, Martin https://orcid.org/0000-0001-5490-613X12 University of Leeds https://ror.org/024mrxd33 ror Chakraborty, Pijush https://orcid.org/0000-0003-1749-522513 University of Leeds https://ror.org/024mrxd33 ror Brinkworth, Alice https://orcid.org/0009-0007-3385-578414 University of Leeds https://ror.org/024mrxd33 ror Willis, Leon https://orcid.org/0000-0001-6616-371615 University of Leeds https://ror.org/024mrxd33 ror Zhuravleva, Anastasia https://orcid.org/0000-0001-6165-850916 University of Leeds https://ror.org/024mrxd33 ror Ranson, Neil A. https://orcid.org/0000-0002-3640-527517 University of Leeds https://ror.org/024mrxd33 ror Foster, Richard https://orcid.org/0000-0002-2361-388418 University of Leeds https://ror.org/024mrxd33 ror Radford, Sheena E. https://orcid.org/0000-0002-3079-803919 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: Wellcome, MRC, EPSRC, Royal Society Grant numbers: Wellcome 204963, MRC MR/T011149/1, EPSRC APP12867, Royal Society RSRP\R1\211057 Publication date: 2025 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: a.taylor10@leeds.ac.uk, s.e.radford@leeds.ac.uk20 Last Modified: 06 Mar 2025 14:38

Files

Documentation

README_Taylor_2025.txt [3kB] 21 README_Taylor_2025.txt [3kB] 22 README_Taylor_2025.txt [3kB] 23 Security: Anyone File name: README_Taylor_2025.txt24 Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 3kB

Data

ThT kinetics for the preliminary SAR (Fig. S1) [30kB] 25 ThT kinetics for the preliminary SAR (Fig. S1) [30kB] 26 ThT kinetics for the preliminary SAR (Fig. S1) [30kB] 27 Security: Anyone File name: 1_ThT_preliminary_SAR.csv28 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 30kB

ROCS searches against YX-I-1 enantiomer 1 [538kB] 29 ROCS searches against YX-I-1 enantiomer 1 [538kB] 30 ROCS searches against YX-I-1 enantiomer 1 [538kB] 31 Security: Anyone File name: 2_ROCS_enantiomer_1.csv32 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 538kB

ROCS searches against YX-I-1 enantiomer 2 [534kB] 33 ROCS searches against YX-I-1 enantiomer 2 [534kB] 34 ROCS searches against YX-I-1 enantiomer 2 [534kB] 35 Security: Anyone File name: 3_ROCS_enantiomer_2.csv36 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 534kB

Compound solubility screening by absorbance spectroscopy (Fig. S3) [149kB] 37 Compound solubility screening by absorbance spectroscopy (Fig. S3) [149kB] 38 Compound solubility screening by absorbance spectroscopy (Fig. S3) [149kB] 39 Security: Anyone File name: 4_absorbance_compound_solubility.csv40 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 149kB

Compound solubility screening by Capflex (Fig. S3) [565kB] 41 Compound solubility screening by Capflex (Fig. S3) [565kB] 42 Compound solubility screening by Capflex (Fig. S3) [565kB] 43 Security: Anyone File name: 5_Capflex_compound_solubility.csv44 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 565kB

Additional TDA experiments to confirm compound solubility (Fig. S4) [1MB] 45 Additional TDA experiments to confirm compound solubility (Fig. S4) [1MB] 46 Additional TDA experiments to confirm compound solubility (Fig. S4) [1MB] 47 Security: Anyone File name: 6_TDA_compound_solubility.csv48 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

ThT kinetics to investigate the assembly mechanism of IAPP (Fig. S5) [92kB] 49 ThT kinetics to investigate the assembly mechanism of IAPP (Fig. S5) [92kB] 50 ThT kinetics to investigate the assembly mechanism of IAPP (Fig. S5) [92kB] 51 Security: Anyone File name: 7_ThT_assembly_mechanism.csv52 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 92kB

ThT kinetics to screen for inhibitors of IAPP assembly (Fig. 2, Fig. S6) [99kB] 53 ThT kinetics to screen for inhibitors of IAPP assembly (Fig. 2, Fig. S6) [99kB] 54 ThT kinetics to screen for inhibitors of IAPP assembly (Fig. 2, Fig. S6) [99kB] 55 Security: Anyone File name: 8_ThT_kinetic_screening.csv56 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 99kB

ThT kinetics to test the sequence-specificity of compounds (Fig. S7) [8kB] 57 ThT kinetics to test the sequence-specificity of compounds (Fig. S7) [8kB] 58 ThT kinetics to test the sequence-specificity of compounds (Fig. S7) [8kB] 59 Security: Anyone File name: 9_ThT_sequence_specificity.csv60 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8kB

ThT kinetics of IAPP at varying compound concentrations (Fig. 3) [115kB] 61 ThT kinetics of IAPP at varying compound concentrations (Fig. 3) [115kB] 62 ThT kinetics of IAPP at varying compound concentrations (Fig. 3) [115kB] 63 Security: Anyone File name: 10_ThT_compound_dependence.csv64 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 115kB

ThT kinetics for pelleting of IAPP at intermediate assembly stages (Fig. S9) [20kB] 65 ThT kinetics for pelleting of IAPP at intermediate assembly stages (Fig. S9) [20kB] 66 ThT kinetics for pelleting of IAPP at intermediate assembly stages (Fig. S9) [20kB] 67 Security: Anyone File name: 11_ThT_intermediate_pelleting.csv68 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 20kB

HPLC analysis for pelleting of IAPP at intermediate assembly stages (Fig. S9) [527kB] 69 HPLC analysis for pelleting of IAPP at intermediate assembly stages (Fig. S9) [527kB] 70 HPLC analysis for pelleting of IAPP at intermediate assembly stages (Fig. S9) [527kB] 71 Security: Anyone File name: 12_HPLC_intermediate_pelleting.csv72 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 527kB

SPR experiments to evaluate binding of compounds to IAPP monomer (Fig. 4) [228kB] 73 SPR experiments to evaluate binding of compounds to IAPP monomer (Fig. 4) [228kB] 74 SPR experiments to evaluate binding of compounds to IAPP monomer (Fig. 4) [228kB] 75 Security: Anyone File name: 13_SPR_monomer_binding.csv76 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 228kB

SPR titration of canagliflozin against IAPP monomer (Fig. S11) [313kB] 77 SPR titration of canagliflozin against IAPP monomer (Fig. S11) [313kB] 78 SPR titration of canagliflozin against IAPP monomer (Fig. S11) [313kB] 79 Security: Anyone File name: 14_SPR_canagliflozin_titration.csv80 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 313kB

NMR data for the paper (Fig. 4, Fig. 5, Fig. S12) [10MB] 81 NMR data for the paper (Fig. 4, Fig. 5, Fig. S12) [10MB] 82 NMR data for the paper (Fig. 4, Fig. 5, Fig. S12) [10MB] 83 Security: Anyone File name: 15_NMR_data.zip84 Content type: Data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 10MB

Absorbance spectra for co-pelleting of fibrils and compounds (Fig. 6) [51kB] 85 Absorbance spectra for co-pelleting of fibrils and compounds (Fig. 6) [51kB] 86 Absorbance spectra for co-pelleting of fibrils and compounds (Fig. 6) [51kB] 87 Security: Anyone File name: 16_absorbance_compound_pelleting.csv88 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 51kB

Capflex data showing binding of compounds to fibrils (Fig. 6) [140kB] 89 Capflex data showing binding of compounds to fibrils (Fig. 6) [140kB] 90 Capflex data showing binding of compounds to fibrils (Fig. 6) [140kB] 91 Security: Anyone File name: 17_Capflex_compound_pelleting.csv92 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 140kB

Fibril crossover distributions from cryo-EM (Fig. 7) [909B] 93 Fibril crossover distributions from cryo-EM (Fig. 7) [909B] 94 Fibril crossover distributions from cryo-EM (Fig. 7) [909B] 95 Security: Anyone File name: 18_EM_fibril_crossovers.csv96 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 909B

Fibril subunit fold distributions from cryo-EM (Fig. 8) [167B] 97 Fibril subunit fold distributions from cryo-EM (Fig. 8) [167B] 98 Fibril subunit fold distributions from cryo-EM (Fig. 8) [167B] 99 Security: Anyone File name: 19_EM_fibril_subunit_folds.csv100 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 167B

ThT kinetics of seeded IAPP assembly (Fig. S17) [21kB] 101 ThT kinetics of seeded IAPP assembly (Fig. S17) [21kB] 102 ThT kinetics of seeded IAPP assembly (Fig. S17) [21kB] 103 Security: Anyone File name: 20_ThT_seeded_aggregation.csv104 Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 21kB

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