Research Data Leeds Repository

Citation

Vafaee, Tayyebeh and Campbell, Fiona and Thomas, Daniel and Roderjan, João G. and Lopes, Sergio V. and da Costa, Francisco D. A. and Desai, Amisha and Rooney, Paul and Jennings, Louise and Fisher, John and Berry, Helen and Ingham, Eileen (2022) Implantation of decellularized porcine pulmonary heart valves in sheep - dataset. University of Leeds. [Dataset] https://doi.org/10.5518/1102

Dataset description

Data on the in vivo performance of acellular porcine pulmonary heart valve roots in the right ventricular outflow tract of juvenile sheep (sheep weights, ultrasonography analysis). Data on the explanted acellular porcine roots at 1 month, 3 months and 12 months plus data on non-implanted ovine pulmonary roots and implanted ovine pulmonary roots at 12 months including: gross analysis, biomechanical properties, histological analysis, histomorphometric analysis of cellular population using a range of antibody markers, quantitative calcium analysis.

Subjects:	H000 - Engineering > H100 - General engineering > H160 - Bioengineering, biomedical engineering & clinical engineering
Divisions:	Faculty of Biological Sciences > Institute of Medical and Biological Engineering Faculty of Engineering and Physical Sciences > Institute of Medical and Biological Engineering Faculty of Engineering and Physical Sciences > School of Mechanical Engineering > Institute of Medical and Biological Engineering
Related resources:	Location Type https://doi.org/10.1177/20417314221102680 Publication https://eprints.whiterose.ac.uk/186778/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	01 Jun 2022 20:07
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/973
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Vafaee, Tayyebeh https://orcid.org/0000-0002-0733-9803 University of Leeds https://ror.org/024mrxd33 ror Campbell, Fiona https://orcid.org/0000-0001-7600-0822 University of Leeds https://ror.org/024mrxd33 ror Thomas, Daniel https://orcid.org/0000-0001-5584-9114 University of Leeds https://ror.org/024mrxd33 ror Roderjan, João G. https://orcid.org/0000-0003-4705-5837 Pontifícia Universidade Católica do Paraná https://ror.org/02x1vjk79 ror Lopes, Sergio V. Pontifícia Universidade Católica do Paraná https://ror.org/02x1vjk79 ror da Costa, Francisco D. A. Pontifícia Universidade Católica do Paraná https://ror.org/02x1vjk79 ror Desai, Amisha https://orcid.org/0000-0001-8611-9958 University of Leeds https://ror.org/024mrxd33 ror Rooney, Paul https://orcid.org/0000-0003-3724-5717 NHS Blood & Transplant Tissue & Eye Services https://ror.org/0227qpa16 ror Jennings, Louise https://orcid.org/0000-0003-1446-4511 University of Leeds https://ror.org/024mrxd33 ror Fisher, John https://orcid.org/0000-0003-3833-3700 University of Leeds https://ror.org/024mrxd33 ror Berry, Helen https://orcid.org/0000-0003-2426-1881 University of Leeds https://ror.org/024mrxd33 ror Ingham, Eileen https://orcid.org/0000-0002-9757-3045 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: Wellcome Trust & EPSRC, EPSRC Grant numbers: WT 088908/Z/09/Z, EP/N00941X/1 Publication date: 2022 Resource language: English Metadata language: English Research project title: PoC015 “In vivo evaluation of acellular porcine pulmonary roots”. Publisher: University of Leeds Contact email: e.ingham@leeds.ac.uk Last Modified: 03 Sep 2024 12:11

Files

Documentation

Ethical considerations [140kB] Ethical considerations [140kB] Ethical considerations [140kB] Security: Anyone File name: ARRIVE_2_completed.pdf Content type: Documentation Description: Completed copy of ARRIVE 2 guidelines Mime-Type: application/pdf License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 140kB

Explanation of valve and image files labelling system [277kB] Explanation of valve and image files labelling system [277kB] Explanation of valve and image files labelling system [277kB] Security: Anyone File name: Explanation_valve_block_section_labelling_system.pdf Content type: Documentation Description: This document file explains the labelling system used for the heart valves, histology blocks and sections created. It explains the labels on .tiff images Mime-Type: application/pdf License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 277kB

Explanation of cell counting analysis [802kB] Explanation of cell counting analysis [802kB] Explanation of cell counting analysis [802kB] Security: Anyone File name: Explanation_Cell_counting_analysis.docx Content type: Documentation Mime-Type: application/vnd.openxmlformats-officedocument.wordprocessingml.document License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 802kB

Data

Summary of data from implanted sheep [271kB] Summary of data from implanted sheep [271kB] Summary of data from implanted sheep [271kB] Security: Anyone File name: Sheep_data_summary.zip Content type: Data Description: Contains three .pdf files of data from the in vivo phase of the sheep study indicating (1) the weight of the sheep over time (2) the ultrasonography data over time and (3) the results of the gross analysis of the heart valves at explantation and an .xlsx Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 271kB

Calcium analysis [13kB] Calcium analysis [13kB] Calcium analysis [13kB] Security: Anyone File name: Quantitative_Calcium_analysis_data.xlsx Content type: Data Description: Raw data on calcium content of non-implanted sheep (ovine NI), non-implanted decellularised porcine (dCELL NI) and explanted valves together with analysis of the data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 13kB

Histology and immunohistochemistry images of non-implanted sheep pulmonary heart valves [1GB] Histology and immunohistochemistry images of non-implanted sheep pulmonary heart valves [1GB] Histology and immunohistochemistry images of non-implanted sheep pulmonary heart valves [1GB] Security: Anyone File name: Non_Implanted_sheep_valves_Histology_IHC.zip Content type: Data Description: The .zip folder contains 14 subfolders (aSMA, CD34, CD80, CD163, CD271, CTGF, H&E, Ki67, Mac, Massons Trichrome, Sirius Red Millers, vimentin, von Kossa, VWF) each containing representative .tiff images of stained sections Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1GB

Histology and immunohistochemistry images of decellularised porcine pulmonary valves explanted from sheep at 1 month [1GB] Histology and immunohistochemistry images of decellularised porcine pulmonary valves explanted from sheep at 1 month [1GB] Histology and immunohistochemistry images of decellularised porcine pulmonary valves explanted from sheep at 1 month [1GB] Security: Anyone File name: Month_1_acellular_porcine_explants_Histology_IHC_V2.zip Content type: Data Description: The .zip folder contains 16 subfolders (aSMA, CD3, CD19, CD34, CD80, CD163, CD271, CTGF, H&E, Ki67, Mac, Massons Trichrome, Sirius Red Millers, vimentin, von Kossa, VWF) each containing representative .tiff images of stained sections Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1GB

Histology and immunohistochemistry images of decellularised porcine pulmonary valves explanted from sheep at 3 months [1GB] Histology and immunohistochemistry images of decellularised porcine pulmonary valves explanted from sheep at 3 months [1GB] Histology and immunohistochemistry images of decellularised porcine pulmonary valves explanted from sheep at 3 months [1GB] Security: Anyone File name: Month3_acellular_porcine_explants_Histology_IHC.zip Content type: Data Description: The .zip folder contains 16 subfolders (aSMA, CD3, CD19, CD34, CD80, CD163, CD271, CTGF, H&E, Ki67, Mac, Massons Trichrome, Sirius Red Millers, vimentin, von Kossa, VWF) each containing representative .tiff images of stained sections Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1GB

Histology and immunohistochemistry images of decellularised porcine pulmonary valves explanted from sheep at 12 months [1GB] Histology and immunohistochemistry images of decellularised porcine pulmonary valves explanted from sheep at 12 months [1GB] Histology and immunohistochemistry images of decellularised porcine pulmonary valves explanted from sheep at 12 months [1GB] Security: Anyone File name: Month12_acellular_porcine_explants_Histology_IHC.zip Content type: Data Description: The .zip folder contains 16 subfolders (aSMA, CD3, CD19, CD34, CD80, CD163, CD271, CTGF, H&E, Ki67, Mac, Massons Trichrome, Sirius Red Millers, vimentin, von Kossa, VWF) each containing representative .tiff images of stained sections Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1GB

Histology and immunohistochemistry images of ovine allograft (homograft) pulmonary valves explanted from sheep at 12 months [1GB] Histology and immunohistochemistry images of ovine allograft (homograft) pulmonary valves explanted from sheep at 12 months [1GB] Histology and immunohistochemistry images of ovine allograft (homograft) pulmonary valves explanted from sheep at 12 months [1GB] Security: Anyone File name: Month12_ovine_homograft_explants_Histology_IHC.zip Content type: Data Description: The .zip folder contains 16 subfolders (aSMA, CD3, CD19, CD34, CD80, CD163, CD271, CTGF, H&E, Ki67, Mac, Massons Trichrome, Sirius Red Millers, vimentin, von Kossa, VWF) each containing representative .tiff images of stained sections Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1GB

Cell counting analysis [1MB] Cell counting analysis [1MB] Cell counting analysis [1MB] Security: Anyone File name: Cell_counting_analysis_Final_V2.zip Content type: Data Description: This.zip folder contains 10 .xlsx files (CD3, CD19, CD34, CD80, CD163, CD271, CTGF, DAPI, Ki67, MAC) which provide the raw data and analysis for the number of cells stained positive for each marker in the control and explanted valves Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

Biomechanical testing [193kB] Biomechanical testing [193kB] Biomechanical testing [193kB] Security: Anyone File name: Material_properties_all_valve_groups_analysis.xlsx Content type: Data Description: Data on the material properties of porcine pulmonary valves and cellular ovine pulmonary valves and analysis of the data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 193kB

Cell counting images Month1 acellular porcine explants [2GB] Cell counting images Month1 acellular porcine explants [2GB] Cell counting images Month1 acellular porcine explants [2GB] Security: Anyone File name: Cell_counting_images_Month1_acellular_porcine_explants.zip Content type: Data Description: Images used for cell counting analysis Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2GB

Cell counting images Month3 acellular porcine explants [2GB] Cell counting images Month3 acellular porcine explants [2GB] Cell counting images Month3 acellular porcine explants [2GB] Security: Anyone File name: Cell_counting_images_Month3_acellular_porcine_explants.zip Content type: Data Description: Images used for cell counting analysis Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2GB

Cell counting images Month12 acellular porcine explants [2GB] Cell counting images Month12 acellular porcine explants [2GB] Cell counting images Month12 acellular porcine explants [2GB] Security: Anyone File name: Cell_counting_images_Month12_acellular_porcine_explants.zip Content type: Data Description: Images used for cell counting analysis Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2GB

Cell counting images Month12 ovine homograft control explants [2GB] Cell counting images Month12 ovine homograft control explants [2GB] Cell counting images Month12 ovine homograft control explants [2GB] Security: Anyone File name: Cell_counting_images_Month12_ovine_homograft_control_explants.zip Content type: Data Description: Images used for cell counting analysis Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2GB

Cell counting images Non implanted ovine controls [3GB] Cell counting images Non implanted ovine controls [3GB] Cell counting images Non implanted ovine controls [3GB] Security: Anyone File name: Cell_counting_images_Non_implanted_ovine_controls.zip Content type: Data Description: Images used for cell counting analysis Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 3GB