Research Data Leeds Repository

Modeling the Mechanical Stiffness of Pancreatic Ductal Adenocarcinoma: dataset

Kpeglo, Delanyo (2022) Modeling the Mechanical Stiffness of Pancreatic Ductal Adenocarcinoma: dataset. University of Leeds. [Dataset] https://doi.org/10.5518/952

Dataset description

The dataset are of raw images and collated data. The raw images are of bright field images and confocal images of the PDAC spheroid cultures with and without TGF-β supplement.The collated data are of the size of the cultures, the oscillatory shear rheology data of the mechanical stiffness assessment of the cultures, and results of efficacy of gemicitabine on the mechanically stiff PDAC cultures.

Keywords:	Tissue mechanics; Tumour microenvironment; Pancreatic ductal adenocarcinoma; Pancreatic stellate cells, Transformnig growth factor β1; Oscillatory shear rheology
Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry
Related resources:	Location Type https://doi.org/10.1016/j.mbplus.2022.100109 Publication https://eprints.whiterose.ac.uk/190100/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	17 Mar 2022 16:29
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/944
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Kpeglo, Delanyo https://orcid.org/0000-0001-8803-5264 d.kpeglo@leeds.ac.uk University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: EPSRC Grant numbers: EP/P023266/1 Publication date: 2022 Resource language: English Metadata language: English Publisher: University of Leeds Contact Email Address: pydk@leeds.ac.uk Last Modified: 01 Mar 2024 11:04

Files

Documentation

List of files [114kB] List of files [114kB] List of files [114kB] Visible to: Anyone File name: list_of_files.rtf Content type: Documentation Mime-Type: application/rtf License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 114kB

Data

Figure 1 and Table S1 [224MB] Figure 1 and Table S1 [224MB] Figure 1 and Table S1 [224MB] Visible to: Anyone File name: Figure_1_and_Table_S1.zip Content type: Data Description: Images, size and doubling time of the spheroids Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 224MB

Figure 2 [3MB] Figure 2 [3MB] Figure 2 [3MB] Visible to: Anyone File name: Figure_2.zip Content type: Data Description: Confocal images of PDAC spheroids Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 3MB

Figure 3, 4 and S4-S9 [3MB] Figure 3, 4 and S4-S9 [3MB] Figure 3, 4 and S4-S9 [3MB] Visible to: Anyone File name: Figure_3_4_and_S4-S9.zip Content type: Data Description: Rheology data for the cultures with and without TGF-β1 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 3MB

Figure 5 and S10-S12 [626MB] Figure 5 and S10-S12 [626MB] Figure 5 and S10-S12 [626MB] Visible to: Anyone File name: Figure_5_and_S10-S12.zip Content type: Data Description: Confocal images of PDAC cultures stained for collagen Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 626MB

Figure 6 [85MB] Figure 6 [85MB] Figure 6 [85MB] Visible to: Anyone File name: Figure_6.zip Content type: Data Description: Confocal images of PDAC cultures stained for HIF-1 alpha and efficay of gemcitabine Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 85MB

Figure S1 and Table S1 [299MB] Figure S1 and Table S1 [299MB] Figure S1 and Table S1 [299MB] Visible to: Anyone File name: Figure_S1_and_Table_S1.zip Content type: Data Description: Images of the spheroids with and without TGF-β1 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 299MB

Figure S2 [35MB] Figure S2 [35MB] Figure S2 [35MB] Visible to: Anyone File name: Figure_S2.zip Content type: Data Description: Confocal images Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 35MB

Figure S3 [11kB] Figure S3 [11kB] Figure S3 [11kB] Visible to: Anyone File name: Figure_S3.zip Content type: Data Description: ATP viabilty assessment of spheroids Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 11kB