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Evaluating Phospholipid-Functionalized Gold Nanorods for in vivo Applications
Citation
Roach, Lucien and Booth, Mary E. and Ingram, Nicola and Paterson, Daniel A. and Batchelor, Damien V. B. and Moorcroft, Samuel C. T. and Bushby, Richard J. and Coletta, P. Louise and Critchley, Kevin and Evans, Stephen D. (2020) Evaluating Phospholipid-Functionalized Gold Nanorods for in vivo Applications. University of Leeds. [Dataset] https://doi.org/10.5518/922
Dataset description
Gold nanorods (AuNRs) have attracted a great deal of attention due to their potential for use in a wide range of biomedical applications. However, their production typically requires the use of the relatively toxic cationic surfactant cetyltrimethylammonium bromide (CTAB) leading to continued demand for protocols to detoxify them for in vivo applications. In this study, a robust and facile protocol for the displacement of CTAB from the surface of AuNRs using phospholipids is presented. After the displacement, CTAB was not detectable by NMR spectroscopy, SERS, or using pH-dependent ζ-potential measurements. The phospholipid functionalized AuNRs demonstrated superior stability and biocompatibility (IC50 > 200 μg⋅mL−1) compared to both CTAB and polyelectrolyte functionalized AuNRs and were well tolerated in vivo. Furthermore, they have high NIR absorbance and produce large amounts of heat under NIR illumination, hence such particles are well suited for plasmonic medical applications.
| Keywords: | gold nanorods, phospholipids, biocompatibility, stability, in vivo | ||||||
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| Subjects: | C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C710 - Applied molecular biology, biophysics & biochemistry F000 - Physical sciences > F300 - Physics > F350 - Medical physics |
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| Divisions: | Faculty of Engineering and Physical Sciences > School of Physics and Astronomy | ||||||
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| License: | Creative Commons Attribution 4.0 International (CC BY 4.0) | ||||||
| Date deposited: | 19 Feb 2021 16:24 | ||||||
| URI: | https://archive.researchdata.leeds.ac.uk/id/eprint/816 | ||||||
Files
Documentation
README.txt [10kB]  |
README.txt [10kB] 
README.txt [10kB] Data
A1__NMR__all_spectra [9MB] |
A2__NMR_spectrum__CTAB [538kB] |
A3__NMR_spectrum__Oleate [1MB] |
A4__NMR_spectrum__CTAB-oleate_AuNRs [431kB] |
A5__NMR_spectrum__DOPC [981kB] |
A6__NMR_spectrum__DSPE-mPEG [1MB] |
A7__NMR_spectrum__Phospholipid_AuNRs [974kB] |
B__SERS_spectra [299kB] |
C__zeta_potential_measurements [69kB] |
D1__CTAB-oleate_AuNRs [91kB] |
D2__CTAB-oleate_1mM_free_CTAB [101kB] |
D3__PSS_AuNRs [87kB] |
D4__DOPC_DSPE-mPEG_AuNRs [90kB] |
E__in-vitro_viabilities [12kB] |
F__DIC_and_darkfield_microscopy [534MB] |
G__TEM_cells_AuNRs [165MB] |
H__Haemolysis [756B] |
I__photothermal_experiments [450kB] |
J__ICP-MS [4kB] |
K__CTAB-Oleate_AuNRs [3MB] |
L__CTAB_AuNRs [97MB] |