Research Data Leeds Repository

Data associated with 'A short motif in the N-terminal region of alpha-synuclein plays a critical role in aggregation and function'

Doherty, Ciaran P. A. and Ulamec, Sabine M. and Maya-Martinez, Roberto and Good, Sarah C. and Makepeace, Jemma and Khan, G. Nasir and Oosten-Hawle, Patricija van and Radford, Sheena E. and Brockwell, David J. (2019) Data associated with 'A short motif in the N-terminal region of alpha-synuclein plays a critical role in aggregation and function'. University of Leeds. [Dataset] https://doi.org/10.5518/707

Dataset description

Raw data for the Nature Structural Molecular Biology paper "A short motif in the N-terminal region of alpha-synuclein plays a critical role in aggregation and function”.

For more information see readme.txt

Keywords:	Alpha Synuclein, Parkinson's disease, aggregation, amyloid, PRE, inhibition, NMR
Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry
Divisions:	Faculty of Biological Sciences > Astbury Centre for Structural Molecular Biology
Related resources:	Location Type https://doi.org/10.1038/s41594-020-0384-x Publication http://eprints.whiterose.ac.uk/155980/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	09 Mar 2020 14:47
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/649
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Doherty, Ciaran P. A. https://orcid.org/0000-0001-5685-4716 Ulamec, Sabine M. Maya-Martinez, Roberto https://orcid.org/0000-0002-7371-8051 Good, Sarah C. https://orcid.org/0000-0002-2263-0690 Makepeace, Jemma Khan, G. Nasir Oosten-Hawle, Patricija van https://orcid.org/0000-0002-5713-6457 Radford, Sheena E. https://orcid.org/0000-0002-3079-8039 Brockwell, David J. https://orcid.org/0000-0002-0802-5937 Type of data: Dataset Research funders: BBSRC, ERC, Wellcome Trust, N3CR Grant numbers: BBSRC (BB/K02101X/1, BB/M011151/1); ERC (322408 ); Wellcome Trust (204963, 215062/Z/18/Z, 094232); N3CR (NC/P001203/1) Publication date: 2019 Resource language: English Publisher: University of Leeds Contact Email Address: C.P.Doherty@leeds.ac.uk Last Modified: 10 Jun 2020 17:11

Files

Documentation

readme.txt [2kB] readme.txt [2kB] readme.txt [2kB] Visible to: Anyone File name: readme.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2kB

Data

Fig 1 aggregation propensity [14kB] Fig 1 aggregation propensity [14kB] Fig 1 aggregation propensity [14kB] Visible to: Anyone File name: 1_Fig1-aggregation_propensity.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 14kB

Fig 2 ThT rawdata [270kB] Fig 2 ThT rawdata [270kB] Fig 2 ThT rawdata [270kB] Visible to: Anyone File name: 2_fig2-ThT-rawdata.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 270kB

Fig 3 disulphide locked dimers ThT [315kB] Fig 3 disulphide locked dimers ThT [315kB] Fig 3 disulphide locked dimers ThT [315kB] Visible to: Anyone File name: 3_Fig3_disulphide_locked_dimers_ThT.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 315kB

Fig 4 PRE-WT [113kB] Fig 4 PRE-WT [113kB] Fig 4 PRE-WT [113kB] Visible to: Anyone File name: 4_Fig4-PRE-WT.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 113kB

Fig 5 PRE-dd [36kB] Fig 5 PRE-dd [36kB] Fig 5 PRE-dd [36kB] Visible to: Anyone File name: 5_Fig5-PRE-dd.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 36kB

Fig 6 aggregatesFRAP [70kB] Fig 6 aggregatesFRAP [70kB] Fig 6 aggregatesFRAP [70kB] Visible to: Anyone File name: 6_Fig6-aggregatesFRAP.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 70kB

Fig 6 motility data [22kB] Fig 6 motility data [22kB] Fig 6 motility data [22kB] Visible to: Anyone File name: 7_Fig6-motility_data.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 22kB

Fig 7 liposome CD [36kB] Fig 7 liposome CD [36kB] Fig 7 liposome CD [36kB] Visible to: Anyone File name: 8_Fig7-liposome_CD.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 36kB

Fig 8 ThT [365kB] Fig 8 ThT [365kB] Fig 8 ThT [365kB] Visible to: Anyone File name: 9_Fig8-ThT.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 365kB

Fig 8 liposomes HSQC [20kB] Fig 8 liposomes HSQC [20kB] Fig 8 liposomes HSQC [20kB] Visible to: Anyone File name: 10_Fig8-liposomes-HSQC.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 20kB

Fig S1 ThT-all [801kB] Fig S1 ThT-all [801kB] Fig S1 ThT-all [801kB] Visible to: Anyone File name: 11_FigS1-ThT-all.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 801kB

Fig S2-seeding [90kB] Fig S2-seeding [90kB] Fig S2-seeding [90kB] Visible to: Anyone File name: 12_FigS2-seeding.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 90kB

Fig S3-ThT control [243kB] Fig S3-ThT control [243kB] Fig S3-ThT control [243kB] Visible to: Anyone File name: 13_FigS3-ThT_control.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 243kB

Figure S5, intensity ratios of paramagnetic: diamagnetic data from PRE NMR experiments for alpha-synuclein P1P2GS variant, presented as XY data. [19kB] Figure S5, intensity ratios of paramagnetic: diamagnetic data from PRE NMR experiments for alpha-synuclein P1P2GS variant, presented as XY data. [19kB] Figure S5, intensity ratios of paramagnetic: diamagnetic data from PRE NMR experiments for alpha-synuclein P1P2GS variant, presented as XY data. [19kB] Visible to: Anyone File name: 14_FigS5_P1P2-GS_PRE.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 19kB

Figure S6, HN-Γ2 rates for WT aSyn labelled with MTSL at position 40 (A40C) and WT and delta delta variants labelled with MTSL at position 129 (A129C). [19kB] Figure S6, HN-Γ2 rates for WT aSyn labelled with MTSL at position 40 (A40C) and WT and delta delta variants labelled with MTSL at position 129 (A129C). [19kB] Figure S6, HN-Γ2 rates for WT aSyn labelled with MTSL at position 40 (A40C) and WT and delta delta variants labelled with MTSL at position 129 (A129C). [19kB] Visible to: Anyone File name: 14_FigS5_P1P2-GS_PRE.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 19kB

Figure S7, see figure 7 for raw CD MRE data. Raw DLS data presented as XY data. [10kB] Figure S7, see figure 7 for raw CD MRE data. Raw DLS data presented as XY data. [10kB] Figure S7, see figure 7 for raw CD MRE data. Raw DLS data presented as XY data. [10kB] Visible to: Anyone File name: 16_FigS7-liposome-DLS.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 10kB

Figure 17 [16MB] Figure 17 [16MB] Figure 17 [16MB] Visible to: Anyone File name: 17_methods-MS-data.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 16MB