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Data associated with 'Cryopreservation of primary cultures of mammalian somatic cells in 96-well plates benefits from control of ice nucleation'

Daily, Martin I. and Whale, Thomas F. and Partanen, Riitta and Harrison, Alexander D. and Kilbride, Peter and Lamb, Stephen and Morris, G. John and Picton, Helen M. and Murray, Benjamin J. (2020) Data associated with 'Cryopreservation of primary cultures of mammalian somatic cells in 96-well plates benefits from control of ice nucleation'. University of Leeds. [Dataset] https://doi.org/10.5518/782

Dataset description

Cryopreservation of mammalian cells has, to date, typically been conducted in cryovials, but there are applications where cryopreservation of primary cells in multiwell plates would be advantageous. However excessive supercooling in the small volumes of liquid in each well of the multiwell plates is inevitable without intervention and tends to result in high and variable cell mortality. We describe a technique for cryopreservation of adhered primary bovine granulosa cells in 96-well plates by controlled rate freezing using controlled nucleation. This dataset contains experimental data derived from granulosa cell culture cryopreservation experiments and measurements of ice nucleation temperature measurements observed within 96-well plates and other cryocontainers

Divisions: Faculty of Environment > School of Earth and Environment > Institute for Climate and Atmospheric Science
Faculty of Medicine and Health > School of Medicine
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https://doi.org/10.1016/j.cryobiol.2020.02.008Publication
http://eprints.whiterose.ac.uk/157958/Publication
License: Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited: 06 Mar 2020 15:50
URI: http://archive.researchdata.leeds.ac.uk/id/eprint/647

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