Research Data Leeds Repository

Citation

Daily, Martin I. and Whale, Thomas F. and Partanen, Riitta and Harrison, Alexander D. and Kilbride, Peter and Lamb, Stephen and Morris, G. John and Picton, Helen M. and Murray, Benjamin J. (2020) Data associated with 'Cryopreservation of primary cultures of mammalian somatic cells in 96-well plates benefits from control of ice nucleation'. University of Leeds. [Dataset] https://doi.org/10.5518/782

Dataset description

Cryopreservation of mammalian cells has, to date, typically been conducted in cryovials, but there are applications where cryopreservation of primary cells in multiwell plates would be advantageous. However excessive supercooling in the small volumes of liquid in each well of the multiwell plates is inevitable without intervention and tends to result in high and variable cell mortality. We describe a technique for cryopreservation of adhered primary bovine granulosa cells in 96-well plates by controlled rate freezing using controlled nucleation. This dataset contains experimental data derived from granulosa cell culture cryopreservation experiments and measurements of ice nucleation temperature measurements observed within 96-well plates and other cryocontainers

Divisions:	Faculty of Environment > School of Earth and Environment > Institute for Climate and Atmospheric Science Faculty of Medicine and Health > School of Medicine
Related resources:	Location Type https://doi.org/10.1016/j.cryobiol.2020.02.008 Publication http://eprints.whiterose.ac.uk/157958/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	06 Mar 2020 15:50
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/647
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Daily, Martin I. https://orcid.org/0000-0001-8387-1394 University of Leeds https://ror.org/024mrxd33 ror Whale, Thomas F. https://orcid.org/0000-0002-1062-2685 University of Warwick https://ror.org/01a77tt86 ror Partanen, Riitta University of Leeds https://ror.org/024mrxd33 ror Harrison, Alexander D. https://orcid.org/0000-0003-1522-0391 University of Leeds https://ror.org/024mrxd33 ror Kilbride, Peter https://orcid.org/0000-0002-6951-9551 Asymptote https://ror.org/047acnn05 ror Lamb, Stephen Asymptote https://ror.org/047acnn05 ror Morris, G. John Asymptote https://ror.org/047acnn05 ror Picton, Helen M. https://orcid.org/0000-0002-7593-775X University of Leeds https://ror.org/024mrxd33 ror Murray, Benjamin J. https://orcid.org/0000-0002-8198-8131 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: ERC, ERC, Asymptote Ltd (GE Healthcare), NERC, NERC, Leverhulme Trust Grant numbers: 713664, 648661, NE/L002574/1, NE/M010473/1, ECF-2018-127 Publication date: 2020 Resource language: English Metadata language: English Research project title: CryoProtect, MarineIce, PhD Studentship, PhD Studentship, Early Career Fellowship Publisher: University of Leeds Contact email: martin.i.daily1@leeds.ac.uk Last Modified: 23 Jul 2024 12:29

Files

Documentation

readme.txt [1kB] readme.txt [1kB] readme.txt [1kB] Security: Anyone File name: readme.txt Content type: Documentation Mime-Type: text/plain File size: 1kB

Data

Raw cell number data [13kB] Raw cell number data [13kB] Raw cell number data [13kB] Security: Anyone File name: raw_cell_data.csv Content type: Data Description: Raw granulosa culture data consiting of cell counts for individual wells within cell batches Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 13kB

96-well plate freezing temperature data [5kB] 96-well plate freezing temperature data [5kB] 96-well plate freezing temperature data [5kB] Security: Anyone File name: 96wellplate_freezing_temperatures.csv Content type: Data Description: IR-NIPI derived 96-well well freezing temperatures plotted in Fig. 2 of paper Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 5kB

96-well plate temperature log with controlled ice nucleation [19kB] 96-well plate temperature log with controlled ice nucleation [19kB] 96-well plate temperature log with controlled ice nucleation [19kB] Security: Anyone File name: IRNIPI_plate_temperature_log_controlled_nucleation.csv Content type: Data Description: IR-NIPI derived 96-well plate temperature log plotted in Fig 3 where ice nucleation was manualy controlled Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 19kB

96-well plate temperature log with uncontrolled ice nucleation [19kB] 96-well plate temperature log with uncontrolled ice nucleation [19kB] 96-well plate temperature log with uncontrolled ice nucleation [19kB] Security: Anyone File name: IRNIPI_plate_temperature_log_uncontrolled_nucleation.csv Content type: Data Description: IR-NIPI derived 96-well plate temperature log plotted in Fig 3 where ice nucleation was uncontrolled Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 19kB

Cryocontainer purified water freezing temperatures (96-well plates, cryovials and cryobags) [1kB] Cryocontainer purified water freezing temperatures (96-well plates, cryovials and cryobags) [1kB] Cryocontainer purified water freezing temperatures (96-well plates, cryovials and cryobags) [1kB] Security: Anyone File name: cryocontainer_water_freezing_temps.csv Content type: Data Description: new data of freezing temperatures of water in 96-well plate, cryovials and cryobags plotted in Fig. 5 Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1kB

ANOVA and post-hoc analysis results for granulosa batches a-h [6kB] ANOVA and post-hoc analysis results for granulosa batches a-h [6kB] ANOVA and post-hoc analysis results for granulosa batches a-h [6kB] Security: Anyone File name: ANOVA_output_groups.zip Content type: Data Description: output files from ANOVA analysis of the granulosa culture data (Minitab v. 17.1.10) Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 6kB

t-test_all_groups_survial.txt [324B] t-test_all_groups_survial.txt [324B] t-test_all_groups_survial.txt [324B] Security: Anyone File name: t-test_all_groups_survial.txt Content type: Data Description: output file from T-test of survival data for all groups (Minitab v. 17.1.10) Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 324B