Research Data Leeds Repository

Citation

Raveendran, Mukhil and Lee, Andrew J. and Wälti, Christoph and Actis, Paolo (2019) Data associated with ‘Analysis of 2-D DNA Origami with Nanopipettes’. University of Leeds. [Dataset] https://doi.org/10.5518/577

Dataset description

Ion current recordings of DNA origami translocation experiments. Nanopipettes are a useful tool for the detection and analysis of biological molecules at the single-molecule level. Here, we employ nanopipettes fabricated from glass capillaries to identify differently structured 2D DNA origami through the distinctive amplitude and dwell time of the ion current peak resulting from the translocation of the origami through the nanopipette pore. We demonstrate that the ion current peak originating from frame-like DNA origami comprises two individual peaks in contrast to solid tiles of similar size that only lead to a single peak in the ion current. Interestingly, the fine details of the shape of the double peak characteristic of the translocation of DNA origami frames are correlated with the structural features of the DNA origami. In particular, the size of the central cavity governs the lag time between the two constituent peaks of the double-peak. This work demonstrates the ability of glass nano- pipettes to identify and characterize differently structured DNA origami of similar size, advancing the potential of nanopipettes for high-sensitivity single-molecule studies.

Keywords:	DNA nanotechnology; DNA origami; nanopipette; nanopore; single-molecule analysis
Divisions:	Faculty of Engineering and Physical Sciences > School of Electronic and Electrical Engineering
Related resources:	Location Type https://eprints.whiterose.ac.uk/133975/ Publication https://doi.org/10.1002/celc.201800732 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	06 Jan 2020 10:22
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/623
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Raveendran, Mukhil https://orcid.org/0000-0003-3968-3673 University of Leeds https://ror.org/024mrxd33 ror Lee, Andrew J. https://orcid.org/0000-0003-2268-4645 University of Leeds https://ror.org/024mrxd33 ror Wälti, Christoph https://orcid.org/0000-0001-9286-5359 University of Leeds https://ror.org/024mrxd33 ror Actis, Paolo https://orcid.org/0000-0002-7146-1854 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Publication date: 2019 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: elmra@leeds.ac.uk Last Modified: 30 Oct 2024 08:33

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Documentation

readme [760B] readme [760B] readme [760B] Security: Anyone File name: readme.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 760B

Data

Plain tiles: Ion-current data [884MB] Plain tiles: Ion-current data [884MB] Plain tiles: Ion-current data [884MB] Security: Anyone File name: plain_tiles.zip Content type: Data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 884MB

Frames: Ion-current data [1GB] Frames: Ion-current data [1GB] Frames: Ion-current data [1GB] Security: Anyone File name: frames.zip Content type: Data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1GB