Research Data Leeds Repository

Citation

Moorcroft, Samuel C. T. and Roach, Lucien and Ong, Zhan Yuin and Jayne, David and Evans, Stephen D. (2019) Nanoparticle loaded hydrogel for the triggered release of liposomal antimicrobial peptides. University of Leeds. [Dataset] https://doi.org/10.5518/706

Dataset description

This data contains information regarding the development of a liposome encapsulating an antimicrobial peptide (2a-3b) and the protection the liposome provides against proteolytic degradation (4). The data also described the photothermal properties of a gel containing peptide liposomes and Auɴʀs (5a, 5b) as well as their release profiles (5d) and toxicities of the gel and components (6a, 6b). Thedata also describes the use of the gel to treat S. aureus (7a, 8) and P. aeruginosa (7b). The remaining data describe the supplementary materials (S1-S5).

Keywords:	Antimicrobial dressing, hydrogel, lipid coated nanorods, liposomes, antimicrobial peptides, photothermal, stimuli-responsive delivery
Subjects:	F000 - Physical sciences > F300 - Physics > F350 - Medical physics
Divisions:	Faculty of Engineering and Physical Sciences > School of Physics and Astronomy
Related resources:	Location Type https://doi.org/10.1021/acsami.9b22587 Publication https://eprints.whiterose.ac.uk/159754/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	29 Oct 2019 10:43
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/595
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Moorcroft, Samuel C. T. https://orcid.org/0000-0002-4028-2124 University of Leeds https://ror.org/024mrxd33 ror Roach, Lucien https://orcid.org/0000-0002-9166-6662 University of Leeds https://ror.org/024mrxd33 ror Ong, Zhan Yuin https://orcid.org/0000-0001-8666-4382 University of Leeds https://ror.org/024mrxd33 ror Jayne, David https://orcid.org/0000-0002-8725-3283 University of Leeds https://ror.org/024mrxd33 ror Evans, Stephen D. https://orcid.org/0000-0001-8342-5335 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: EPSRC Grant numbers: 1819417 Publication date: 2019 Resource language: English Metadata language: English Research project title: SMART sutures Publisher: University of Leeds Contact email: py12sctm@leeds.ac.uk Last Modified: 30 Oct 2024 11:39

Files

Documentation

Readme.txt [4kB] Readme.txt [4kB] Readme.txt [4kB] Security: Anyone File name: Readme.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 4kB

Data

Liposome calcien leakage [8kB] Liposome calcien leakage [8kB] Liposome calcien leakage [8kB] Security: Anyone File name: Liposome_calcien_leakage.xlsx Content type: Data Description: Calcein leakage from liposomes containing different amounts of cholesterol Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8kB

Liposome calcein release [9kB] Liposome calcein release [9kB] Liposome calcein release [9kB] Security: Anyone File name: Liposome_calcein_release.xlsx Content type: Data Description: Calcein release in the presence of S. aureus and heated to 55C. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 9kB

IK8 liposome encapsulation [9kB] IK8 liposome encapsulation [9kB] IK8 liposome encapsulation [9kB] Security: Anyone File name: IK8_liposome_encapsulation.xlsx Content type: Data Description: Encapsulation of IK8 when varing the intialy concentration of peptide during lipid film hydration. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 9kB

Liposome stability [9kB] Liposome stability [9kB] Liposome stability [9kB] Security: Anyone File name: Liposome_stability_IK8.xlsx Content type: Data Description: Diameter and PDI of liposomes over 3 weeks. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 9kB

Liposome protection of encapsulated IK8 [9kB] Liposome protection of encapsulated IK8 [9kB] Liposome protection of encapsulated IK8 [9kB] Security: Anyone File name: Liposome_protection_of_encapsulated_IK8.xlsx Content type: Data Description: IK8 remaining after incubtation with the proteolytic enzyme trypsin. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 9kB

Varying temperature increase with laser intensity [88kB] Varying temperature increase with laser intensity [88kB] Varying temperature increase with laser intensity [88kB] Security: Anyone File name: Varying_temperature_increase_with_laser_intensity.xlsx Content type: Data Description: The temperature of the gel can be controlled by varyingthe applied laser intensity. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 88kB

Repeated temperature increase [366kB] Repeated temperature increase [366kB] Repeated temperature increase [366kB] Security: Anyone File name: Repeated_temperature_increase.xlsx Content type: Data Description: The repeatable heating profile of the gel under several round of laser irradiation at the same intensity. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 366kB

Calcein release from liposomes in gel [8kB] Calcein release from liposomes in gel [8kB] Calcein release from liposomes in gel [8kB] Security: Anyone File name: Calcein_release_from_liposomes_ingel.xlsx Content type: Data Description: The release of calcein from liposomes embedded within different wt%of gel. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8kB

Gel toxicity [8kB] Gel toxicity [8kB] Gel toxicity [8kB] Security: Anyone File name: Gel_toxicity.xlsx Content type: Data Description: Toxicity of the gel against human dermal fibroblasts Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8kB

IK8 and AuNR toxicity [9kB] IK8 and AuNR toxicity [9kB] IK8 and AuNR toxicity [9kB] Security: Anyone File name: IK8_and_AuNR_toxicity.xlsx Content type: Data Description: Toxicity of the IK8 and AuNRs against human dermal fibroblasts Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 9kB

Treatment of S. aureus [8kB] Treatment of S. aureus [8kB] Treatment of S. aureus [8kB] Security: Anyone File name: Treatment_of_S_aureus.xlsx Content type: Data Description: The log reductions of S. aureus after treatment with the therapeutic gel. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8kB

Treatment of P. aeruginosa [8kB] Treatment of P. aeruginosa [8kB] Treatment of P. aeruginosa [8kB] Security: Anyone File name: Treatment_of_P_aeruginosa.xlsx Content type: Data Description: The log reductions of P. aeruginosa after treatment with the therapeutic gel. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8kB

Multiple treatments of S. aureus [8kB] Multiple treatments of S. aureus [8kB] Multiple treatments of S. aureus [8kB] Security: Anyone File name: Multiple_treatments_of_S_aureus.xlsx Content type: Data Description: The log reductions of multiple rounds of S. aureus after treatment with the therapeutic gel. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8kB

IK8 concentration curve [34kB] IK8 concentration curve [34kB] IK8 concentration curve [34kB] Security: Anyone File name: IK8_concentration_curve.xlsx Content type: Data Description: The HPLC data used to produce a calibration curve of IK8. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 34kB

AuNR UV-vis [10kB] AuNR UV-vis [10kB] AuNR UV-vis [10kB] Security: Anyone File name: AuNR_UV-vis.xlsx Content type: Data Description: UV-vis of AuNRs before and after lipid coating Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 10kB

AAS calibration curve [31kB] AAS calibration curve [31kB] AAS calibration curve [31kB] Security: Anyone File name: AAS_calibration_curve.xlsx Content type: Data Description: The calibration curve obtained using atomic absorbtion spectroscopy and used to quantify the concentration of Au in a AuNR sample. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 31kB

Liposome calcein leakage in buffer, AMP and cells [8kB] Liposome calcein leakage in buffer, AMP and cells [8kB] Liposome calcein leakage in buffer, AMP and cells [8kB] Security: Anyone File name: Liposome_calcein_leakage_in_buffer_AMP_and_cells.xlsx Content type: Data Description: Calcein leakage from liposomes incubated with PBS, 5 mM IK8 and human dermal fibroblasts. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8kB

Sa and Pa MIC [37kB] Sa and Pa MIC [37kB] Sa and Pa MIC [37kB] Security: Anyone File name: Sa_and_Pa_MIC.xlsx Content type: Data Description: Growth of S. aureus and P. aeruginosa when treated with different concentrations of IK8. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 37kB

Liposome calcein release with irradiation time [34kB] Liposome calcein release with irradiation time [34kB] Liposome calcein release with irradiation time [34kB] Security: Anyone File name: Liposome_calcein_release_with_irradiation_time.xlsx Content type: Data Description: Calcein leakage of the gel containing calcein liposomes and AuNRs when irradiated for different time periods. Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 34kB