This readme.txt file was generated on 2019/10/02 by SCT Moorcroft ------------------- GENERAL INFORMATION ------------------- Title of Dataset:Nanoparticle loaded hydrogel for the triggered release of liposomal antimicrobial peptides SCT Moorcroft, University of Leeds, EC Stoner Building, Uni of Leeds, Leeds, LS2 9JT. py12sctm@leeds.ac.uk (Name, Institution, Address, Email) Principal Investigator:Prof. SD Evans Associate or Co-investigator:Prof. DG Jayne, Dr. ZY Ong Alternate Contact(s): Date of data collection: 2017/10/01 - 2019/06/01 Geographic location of data collection: Leeds, West Yorkshire, UK. Information about funding sources or sponsorship that supported the collection of the data:EPSRC funded, grant code 1819417 -------------------- DATA & FILE OVERVIEW -------------------- File list (filenames, directory structure (for zipped files) and brief description of all data files): Liposome calcien leakage.xlsx, Calcein leakage from liposomes containing different amounts of cholesterol Ĺiposome calcein release.xlsx, Calcein release in the presence of S. aureus and heated to 55C. IK8 liposome encapsulation.xlsx, Encapsulation of IK8 when varing the intialy concentration of peptide during lipid film hydration. Liposome stability IK8.xlsx, Diameter and PDI of liposomes over 3 weeks. Liposome protection of encapsulated IK8.xlsx, IK8 remaining after incubtation with the proteolytic enzyme trypsin. Varying temperature increase with laser intensity.xlsx, The temperature of the gel can be controlled by varyingthe applied laser intensity. Repeated temperature increase.xlsx, The repeatable heating profile of the gel under several round of laser irradiation at the same intensity. Calcein release from liposomes in gel.xlsx, The release of calcein from liposomes embedded within different wt%of gel. Gel toxicity.xlsx, Toxicity of the gel against human dermal fibroblasts IK8 and AuNR toxicity.xlsx, Toxicity of the IK8 and AuNRs against human dermal fibroblasts Treatment of S. aureus.xlsx, The log reductions of S. aureus after treatment with the therapeutic gel. Treatment of P. aeruginosa.xlsx, The log reductions of P. aeruginosa after treatment with the therapeutic gel. Multiple treatments of S. aureus.xlsx, The log reductions of P. aeruginosa after treatment with the therapeutic gel. IK8 concentration curve.xlsx, The HPLC data used to produce a calibration curve of IK8. AuNR UV-vis.xlsx, UV-vis of AuNRs before and after lipid coating AAS calibration curve.xlsx, The calibration curve obtained using atomic absorbtion spectroscopy and used to quantify the concentration of Au in a AuNR sample. Liposome calcein leakage in buffer, AMP and cells.xlsx, Calcein leakage from liposomes incubated with PBS, 5 mM IK8 and human dermal fibroblasts. Sa and Pa MIC.xlsx, Growth of S. aureus and P. aeruginosa when treated with different concentrations of IK8. Liposome calcein release with irradiation time.xlsx, Calcein leakage of the gel containing calcein liposomes and AuNRs when irradiated for different time periods. -------------------------- METHODOLOGICAL INFORMATION -------------------------- Description of methods used for collection/generation of data: IK8 encapsulation was quantified using HPLC. Leakage and release assays were performed by measuring the fluorescent response of calcein loaded liposomes. Liposome sizing was performed using DLS. Nanorod characterisation was performed using TEM imaging, AAS and UV-vis spectroscopy. All photothermal heating characterisation was measured through the addition of thermocouples to wells containing nanoparticles under irradiation. Toxicity assays were performed using WST-1. The CFU of bacteria was determined through micro dilution techniques and agar plating. Methods for processing the data: All graphs and data processing was performed using OriginPro 2016. People involved with sample collection, processing, analysis and/or submission: All data was collected by SCT Moorcroft