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Dataset associated with 'Interactions between callose and cellulose revealed through the analysis of biopolymer mixtures'

Abou-Saleh, Radwa H. and Hernandez-Gomez, Mercedes and Amsbury, Sam and Paniagua, Candelas and Bourdon, Matthieu and Miyashima, Shunsuke and Helariutta, Yka and Fuller, Martin and Budtova, Tatiana and Connell, Simon and Ries, Michael E. and Benitez-Alfonso, Yoselin (2018) Dataset associated with 'Interactions between callose and cellulose revealed through the analysis of biopolymer mixtures'. University of Leeds. [Dataset] https://doi.org/10.5518/225

Dataset description

The properties of (1,3)-β-glucans (i.e. callose) remain largely unknown despite their importance in plant development and defence. Mixtures of (1,3)-β-glucan and cellulose, in ionic liquid solution and hydrogels, were used as proxies to understand the physico-mechanical properties of callose in cell walls. With callose addition, the stiffness of callose-cellulose hydrogels was reduced, to a greater extent than predicted from the ideal mixing rule (i.e. the weighted average of the individual components’ properties). In contrast, yield behaviour after the elastic limit is more ductile in cellulose-callose hydrogels compared with sudden failure in 100% cellulose hydrogels. The viscoelastic behaviour and the diffusion of the ions in mixed ionic liquid solutions strongly indicate interactions between the polymers. These interactions are likely linked to changes in cellulose organization in the presence of callose recorded by FTIR analysis of hydrogels and transgenic cell walls. The implications in the context of callose biological function are discussed.

Keywords: Cellulose, (1,3)-β-glucans, Callose, Pachyman, Avicel, ionic liquid solutions, cell walls, Hydrogels, Biopolymers, rheology, AFM-nanoindentation, 1H-NMR, polymer interactions, toughness.
Subjects: C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry > C770 - Biophysical science
Divisions: Faculty of Biological Sciences > Centre for Plant Sciences
Related resources:
LocationType
https://doi.org/10.1038/s41467-018-06820-yPublication
http://eprints.whiterose.ac.uk/134699/Publication
License: Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited: 04 Sep 2018 14:12
URI: https://archive.researchdata.leeds.ac.uk/id/eprint/414

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