Research Data Leeds Repository

Citation

Ormiston-Lee, Lucy and Berry, Helen (2025) Development and in vitro assessment of novel decellularised adipose sheets for adipose tissue regeneration paper data. University of Leeds. [Dataset] https://doi.org/10.5518/1645

Dataset description

Background: Decellularised porcine adipose tissue (DAT) is a potential regenerative treatment for adipose tissue regeneration, but decellularisation often involves methods that compromise the integrity of the extracellular matrix architecture. The aim of this study was to develop a protocol to produce a sheet form of DAT and to assess the cellular response to DAT.

Methods: A novel method of producing DAT was first assessed using histology and biochemical assays to validate its success. An extract cytotoxicity assay was used to detect whether DAT was cytotoxic towards two fibroblast cell lines: 3T3 and L929. Following this the DAT was cultured with 3T3 and 3T3-L1 (pre-adipocyte) cell-lines for 28 days. Cell metabolism was analysed, and cell viability and differentiation potential were investigated using live/dead and BODIPY neutral lipid staining. Finally, the angiogenic potential of DAT was assessed using a chick chorioallantoic membrane assay.

Results: DAT was successfully produced in sheet form (n=6) with ~2 % lipid, <0.1 µg / mg sodium dodecyl sulphate and <50 ng DNA/mg of tissue remaining. The DAT was shown to be non-cytotoxic to both 3T3 and L929 and supported the attachment and migration of 3T3 and 3T3-L1 cell lines in vitro. DAT also supported the accumulation of lipids within 3T3-L1 cells and the directional growth of blood vessels ex ovo (n=6 DAT, n=5 CAM).

Conclusions: A suitable method to produce DAT has been developed which results in DAT sheets that are non-cytotoxic, cytocompatible and have potential to support adipocyte differentiation and angiogenesis in vivo.

Keywords:	Decellularisation, adipose tissue, regenerative materials.
Subjects:	C000 - Biological sciences
Divisions:	Faculty of Biological Sciences > Institute of Medical and Biological Engineering Faculty of Engineering and Physical Sciences > Institute of Medical and Biological Engineering Faculty of Engineering and Physical Sciences > School of Mechanical Engineering > Institute of Medical and Biological Engineering
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	04 Aug 2026 20:07
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/1584
Additional details Additional details Alternative title: Developing a decellularised adipose tissue. Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Ormiston-Lee, Lucy https://orcid.org/0000-0002-0187-5269 lucy.o.lee@gmail.com University of Leeds https://ror.org/024mrxd33 ror Berry, Helen https://orcid.org/0000-0003-2426-1881 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Contributors: Contribution Name ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Ormiston-Lee, Lucy M https://orcid.org/0000-0002-0187-5269 University of Leeds Brockett, Claire L https://orcid.org/0000-0002-6664-7259 University of Leeds Furmidge, Rachel https://orcid.org/0009-0001-1826-6515 University of Sheffield Berry, Helen E https://orcid.org/0000-0003-2426-1881 University of Sheffield Hearnden, Vanessa L https://orcid.org/0000-0003-0838-7783 University of Sheffield Workman, Victoria L https://orcid.org/0000-0002-9135-8993 University of Sheffield Edwards, Jennifer H https://orcid.org/0000-0003-4152-6140 PLOS Research funders: University of Leeds Grant numbers: UoL funded internally Publication date: 2025 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: bs17lo@leeds.ac.uk Last Modified: 05 Aug 2026 07:24

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Data

Figure 1 D-E - DAPI - DAT analysis [217MB] Figure 1 D-E - DAPI - DAT analysis [217MB] Figure 1 D-E - DAPI - DAT analysis [217MB] Security: Anyone File name: Figure-1-2.zip Content type: Data Description: Includes DAPI & H&E histology images, DNA quantification, lipid quantification, SDS quantification, measurements of basement membrane and sterility testing with thioglycolate broth. Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 217MB

Figure 3 - Cytotoxicity [150MB] Figure 3 - Cytotoxicity [150MB] Figure 3 - Cytotoxicity [150MB] Security: Anyone File name: Figure-3.zip Content type: Data Description: Extract cytotoxicity images and ATPlite assay data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 150MB

Figure 4-5 - 3T3 + DAT [523MB] Figure 4-5 - 3T3 + DAT [523MB] Figure 4-5 - 3T3 + DAT [523MB] Security: Anyone File name: Figure-4-5.zip Content type: Data Description: H&E images of 3T3 migration into DAT, alamarblue of 3T3 in DAT, and live dead images/quantification Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 523MB

Figure 6-7 - 3T3-L1 +DAT [188MB] Figure 6-7 - 3T3-L1 +DAT [188MB] Figure 6-7 - 3T3-L1 +DAT [188MB] Security: Anyone File name: Figure-6-7.zip Content type: Data Description: alamarblue of 3T3-L1 in DAT, live dead images/quantification and BODIPY stained images Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 188MB

Figure 8 - CAM [2GB] Figure 8 - CAM [2GB] Figure 8 - CAM [2GB] Security: Anyone File name: Figure-8.zip Content type: Data Description: Chick chorioallantoic membrane assay images, H&E images and analysis of directional vessels/vessel density Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2GB