1. ABOUT THE DATASET
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Title:	Development and in vitro assessment of novel decellularised adipose sheets for adipose tissue regeneration paper data

Creator(s): L M. Ormiston-Lee [1,2], R. Furmidge [3,4], V L. Workman [3], C L. Brockett [4,5], J H. Edwards [1,2], V L. Hearnden [3,4], H E. Berry [1,2]

Organisation(s): 1. School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, UK, LS2 9JT
2. Institute of Medical and Biological Engineering, University of Leeds, Leeds, UK, LS2 9JT
3. Department of Chemical, Material and Biological Engineering, University of Sheffield, Sheffield, UK, S1 3JD
4. INSIGNEO institute, University of Sheffield, Sheffield, UK, S1 3JD
5. Department of Mechanical Engineering, University of Sheffield, Sheffield, UK, S1 3JD


Rights-holder(s): Copyright 2026 University of Leeds

Publication Year: 2026

Description: Characterisation of decellularised porcine adipose tissue (DAT). DAPI & H&E staining of DAT and native adipose tissue (NAT). DNA quantification of DAT and NAT, measured using DNA extraction with DNeasy kit and measurement with Quantit pico green assay. Lipid quantification of free lipids in DAT and NAT. Measurement of the basement membrane gaps in the DAT compared to adipocytes in NAT, measured using imageJ. SDS quantification in DAT using a stainsALL assay. Thioglycolate broth and DAT incubation images showing sterility. Extract cytotoxicity assay in 3T3 and L929 cells, data showing phase-contrast images of cell morphology and ATPlite assay. H&E images showing migration of 3T3 cells into DAT over 28 days. Alamarblue metabolism, live/dead images and quantification of proportion of live-dead cells of 3T3 and 3T3-L1 cells grown in DAT. BODIPY staining of 3T3-L1 cells in DAT after 28 days showing adipocyte differentiation. Chick chorioallantoic membrane (CAM) assay images, H&E images of CAM assay and imageJ analysis of CAM showing directional vessel growth and vessel density.

Cite as: L M. Ormiston-Lee, R. Furmidge, V L. Workman, C L. Brockett, J H. Edwards, V L. Hearnden, H E. Berry (2026) Dataset for 'Development and in vitro assessment of novel decellularised adipose sheets for adipose tissue regeneration'. University of Leeds. [Dataset] https://doi.org/10.5518/1645 

Related publication: L M. Ormiston-Lee, R. Furmidge, V L. Workman, C L. Brockett, J H. Edwards, V L. Hearnden, H E. Berry, Development and in vitro assessment of novel decellularised adipose sheets for adipose tissue regeneration, eCM, 2023 (Submitted; minor reviewer comments).




2. TERMS OF USE
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[A standard copyright notice and licence statement with URL can be used, e.g. Copyright [publication year] [University of Leeds, name of other rights-holder(s)]. Unless otherwise stated, this dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/.]


3. PROJECT AND FUNDING INFORMATION
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Title:  Development and in vitro assessment of novel decellularised adipose sheets for adipose tissue regeneration

Dates: Oct 2021 - Nov 2025

Funding organisation: University of Leeds

Grant no.: N/A

This dataset was not created in the course of a funded project.


4. CONTENTS
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File listing

Figure 1-2 - DAT analysis : Includes DAPI & H&E histology images, DNA quantification, lipid quantification, SDS quantification, measurements of basement membrane and sterility testing with thioglycolate broth.
Figure 3 - Cytotoxicity : Extract cytotoxicity images and ATPlite assay data.
Figure 4-5 - 3T3 + DAT : H&E images of 3T3 migration into DAT, alamarblue of 3T3 in DAT, and live dead images/quantification.
Figure 6-7 - 3T3-L1 +DAT : alamarblue of 3T3-L1 in DAT, live dead images/quantification and BODIPY stained images. 
Figure 8 - CAM : Chick chorioallantoic membrane assay images, H&E images and analysis of directional vessels/vessel density.


5. METHODS
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Refer to the related publication: L M. Ormiston-Lee, R. Furmidge, V L. Workman, C L. Brockett, J H. Edwards, V L. Hearnden, H E. Berry, Development and in vitro assessment of novel decellularised adipose sheets for adipose tissue regeneration, eCM, 2023 (Submitted; minor reviewer comments).