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Data for 'BAM-BepA complexes in outer membrane protein quality control'
Citation
Fenn, Katherine, Higgins, Victoria, Machin, Jonathan, Calabrese, Antonio N., Berry, Alan, Radford, Sheena E. and Ranson, Neil A. (2026) Data for 'BAM-BepA complexes in outer membrane protein quality control'. University of Leeds. [Dataset] https://doi.org/10.5518/1838
Dataset description
Correct folding of outer membrane proteins (OMPs) by the β-barrel assembly machinery (BAM) is essential for maintaining the outer membrane (OM) barrier function of sidearm bacteria. When OMP biogenesis is perburbed, the β-barrel assembly enhancing protease A(BepA) binds to BAM to mediate quality control but how BepA interacts with BAM and degrades substrate OMPs remains unclear. Here we present, AF3 predictions of BepA, BepA with BAM from 11 bacterial species, BepA with 15 residues peptides encompassing the entire sequence of 3 OMPs (BamA, LptD, OmpX) and HPLC data from BepA and BepA mutants degrading a series of OMP peptides.
| Keywords: | Alphafold, BAM, BepA, OMPs, cryoEM | ||||||
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| Subjects: | C000 - Biological sciences > C100 - Biology > C130 - Cell biology C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry |
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| Divisions: | Faculty of Biological Sciences > School of Molecular and Cellular Biology | ||||||
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| License: | Creative Commons Attribution 4.0 International (CC BY 4.0) | ||||||
| Date deposited: | 04 Jun 2026 20:05 | ||||||
| URI: | https://archive.researchdata.leeds.ac.uk/id/eprint/1559 | ||||||
Files
Documentation
readme_Fenn-etal_2026.txt [6kB]  |
readme_Fenn-etal_2026.txt [6kB] 
readme_Fenn-etal_2026.txt [6kB] Data
AF3_BAM_BepA_otherspecies.zip [4GB] |
AF3_BepA.zip [11MB] |
AF3_BepA_peptides.zip [3GB] |
AF3_OtherPlugEnzymes.zip [110MB] |
HPLC_rawdata.zip [4MB] |
AF3_BAM_BepA.pdb [1MB] |