Research Data Leeds Repository

Citation

Byrd, Emily, Crossley, Joel A., Chau, Chalmers C., Actis, Paolo and Calabrese, Antonio N. (2026) An ALS-Associated Mutation in the C-terminal alpha-helix of TDP-43 Uncouples Condensate Formation and Amyloid Assembly. University of Leeds. [Dataset] https://doi.org/10.5518/1785

Dataset description

This data set includes native mass spectrometry and ion mobility mass spectrometry data collected on a Synapt G2 Si HDMS instrument (Waters Corporation at the University of Leeds, processed using MassLynx 4.1. Thioflavin T fluorescence data collected on a FLUOstar omega plate reader (BMG Labtech, Ortenburg, Germany). Nephelometry measurements collected on a Nephelostar plate reader (BMG Labtech, Ortenburg, Germany). SLAB simulations generated using the CALVADOS python package and the CALVADOS2 force field.

Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry C000 - Biological sciences > C100 - Biology > C130 - Cell biology
Divisions:	Faculty of Biological Sciences > School of Molecular and Cellular Biology
Related resources:	Location Type https://doi.org/10.1002/pro.70565 Publication https://eprints.whiterose.ac.uk/id/eprint/239556/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	13 May 2026 20:07
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/1548
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Byrd, Emily https://orcid.org/0000-0002-9876-1400 University of Leeds https://ror.org/024mrxd33 ror Crossley, Joel A. https://orcid.org/0000-0002-6656-1578 University of Leeds https://ror.org/024mrxd33 ror Chau, Chalmers C. https://orcid.org/0000-0002-3134-6798 University of Leeds https://ror.org/024mrxd33 ror Actis, Paolo https://orcid.org/0000-0002-7146-1854 University of Leeds https://ror.org/024mrxd33 ror Calabrese, Antonio N. https://orcid.org/0000-0003-2437-7761 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: Wellcome, BBSRC, Royal Society Grant numbers: 220628/Z/20/Z, BB/Y00034X/1, BB/X003086/1, BB/X003086/1, RGS\R2\222357 Publication date: 2026 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: A.Calabrese@leeds.ac.uk Last Modified: 14 May 2026 08:26

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Documentation

readme_1785.txt [10kB] readme_1785.txt [10kB] readme_1785.txt [10kB] Security: Anyone File name: readme_1785.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 10kB

Data

IM-MS_data.zip [5GB] IM-MS_data.zip [5GB] IM-MS_data.zip [5GB] Security: Anyone File name: IM-MS_data.zip Content type: Data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 5GB

TDP-43_LCD_SLAB_pH55_10us.zip [200MB] TDP-43_LCD_SLAB_pH55_10us.zip [200MB] TDP-43_LCD_SLAB_pH55_10us.zip [200MB] Security: Anyone File name: TDP-43_LCD_SLAB_pH55_10us.zip Content type: Data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 200MB

ThT_Raw.xlsx [224kB] ThT_Raw.xlsx [224kB] ThT_Raw.xlsx [224kB] Security: Anyone File name: ThT_Raw.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 224kB

Nephelometry_CTD_20_170_nacl.xlsx [128kB] Nephelometry_CTD_20_170_nacl.xlsx [128kB] Nephelometry_CTD_20_170_nacl.xlsx [128kB] Security: Anyone File name: Nephelometry_CTD_20_170_nacl.xlsx Content type: Data Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 128kB