Research Data Leeds Repository

Dataset for 'Evaluation of Lipid-Stabilised Tripropionin Nanodroplets as a Delivery Route for Combretastatin A4'

Mico, Victoria and Charalambous, Antonia and Peyman, Sally A. and Abou-Saleh, Radwa H. and Markham, Alexander F. and Coletta, Louise and Evans, Stephen D. (2017) Dataset for 'Evaluation of Lipid-Stabilised Tripropionin Nanodroplets as a Delivery Route for Combretastatin A4'. University of Leeds. [Dataset] https://doi.org/10.5518/98

Dataset description

This data set corresponds to the work titled "Evaluation of Lipid-Stabilised Tripropionin Nanodroplets as a Delivery Route for Combretastatin A4", and contains data on the characterisation of LONDs, specifically sizing, concentration measurements, stability over time and appearance under TEM. Data on the encapsualtion of Combretastatin A4 in tripropionin LONDs is included, as well as in vitro work on the use of these LONDs as vehicle for the delivery of the drug in vitro.

Keywords:	Drug delivery, nanoemulsion, combretastatin
Subjects:	F000 - Physical sciences > F500 - Astronomy > F510 - Astrophysics
Divisions:	Faculty of Engineering and Physical Sciences > School of Physics and Astronomy
Related resources:	Location Type https://doi.org/10.1016/j.ijpharm.2017.05.009 Publication http://eprints.whiterose.ac.uk/116230/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	11 May 2017 11:25
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/148
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Mico, Victoria https://orcid.org/0000-0001-9648-7287 Charalambous, Antonia https://orcid.org/0000-0001-5895-3697 Peyman, Sally A. https://orcid.org/0000-0002-1600-5100 Abou-Saleh, Radwa H. https://orcid.org/0000-0002-8471-2659 Markham, Alexander F. https://orcid.org/0000-0002-6639-3199 Coletta, Louise https://orcid.org/0000-0002-3858-6073 Evans, Stephen D. https://orcid.org/0000-0001-8342-5335 Type of data: Dataset Research funders: EPSRC Collection period: Date from Date to 1 April 2013 30 September 2016 Grant numbers: EP/K023845/1 Publication date: 2017 Resource language: English Research project title: Microbubbles for Hydrophobic Drug Delivery and Enhanced Diagnostics; Towards Personalised Healthcare for the Treatment of Colorectal Cancer Publisher: University of Leeds Contact Email Address: py11vme@leeds.ac.uk Last Modified: 11 May 2017 22:45

Files

Documentation

readme.txt [692B] readme.txt [692B] readme.txt [692B] Visible to: Anyone File name: readme.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 692B

Data

Figure 2 - Changes in size distribution of squalane LONDs [2kB] Figure 2 - Changes in size distribution of squalane LONDs [2kB] Figure 2 - Changes in size distribution of squalane LONDs [2kB] Visible to: Anyone File name: 2squalaneDLS.csv Content type: Data Description: DLS sizing of a squalane LONDs samples, at different production stages Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2kB

Figure 3- Variation of LONDs size with the production pressure [6kB] Figure 3- Variation of LONDs size with the production pressure [6kB] Figure 3- Variation of LONDs size with the production pressure [6kB] Visible to: Anyone File name: 3pressureDLS.csv Content type: Data Description: DLS sizing of squalane and tripropionin LONDs, at different production pressures Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 6kB

Figure 4- Size and concentration changes of tripropionin and squalane LONDs at different production pressures [515B] Figure 4- Size and concentration changes of tripropionin and squalane LONDs at different production pressures [515B] Figure 4- Size and concentration changes of tripropionin and squalane LONDs at different production pressures [515B] Visible to: Anyone File name: 4sizeconcentrationNanosight.csv Content type: Data Description: NTA sizing and concentration measurement of trirpopionin and squalane LONDs, at different production pressures Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 515B

Figure 5a- Stability of LONDs for 6 weeks [8kB] Figure 5a- Stability of LONDs for 6 weeks [8kB] Figure 5a- Stability of LONDs for 6 weeks [8kB] Visible to: Anyone File name: 5astability6.csv Content type: Data Description: DLS sizing of squalane and tripropionin LONDs over 6 weeks Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8kB

Figure 5b - Stability of LONDs at 37C [18kB] Figure 5b - Stability of LONDs at 37C [18kB] Figure 5b - Stability of LONDs at 37C [18kB] Visible to: Anyone File name: 5bstability37.csv Content type: Data Description: DLS sizing of squalane and tripropionin LONDs under physiological conditions during 2h Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 18kB

Figure 6a - TEM squalane LONDs [1MB] Figure 6a - TEM squalane LONDs [1MB] Figure 6a - TEM squalane LONDs [1MB] Visible to: Anyone File name: 6asqualaneLONDs.tif Content type: Data Description: TEM images of squalane LONDs, negatively stained with uranyl acetate Mime-Type: image/tiff License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

Figure 6b - TEM tripropionin LONDs [1MB] Figure 6b - TEM tripropionin LONDs [1MB] Figure 6b - TEM tripropionin LONDs [1MB] Visible to: Anyone File name: 6btripropioninLONDs.tif Content type: Data Description: TEM images of tripropionin LONDs, negatively stained with uranyl acetate Mime-Type: image/tiff License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

Figure 7 - Escalating dose of CA4 LONDs on SVR cells [1MB] Figure 7 - Escalating dose of CA4 LONDs on SVR cells [1MB] Figure 7 - Escalating dose of CA4 LONDs on SVR cells [1MB] Visible to: Anyone File name: 7SVR.jpg Content type: Data Description: Fluorescence confocal microscope images of SVR trated with different doses of tripropionin CA4 LONDs Mime-Type: image/jpeg License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

Figure 8 - Quantification of microtubule length following treatment with CA4 in tripropionin LONDs [11kB] Figure 8 - Quantification of microtubule length following treatment with CA4 in tripropionin LONDs [11kB] Figure 8 - Quantification of microtubule length following treatment with CA4 in tripropionin LONDs [11kB] Visible to: Anyone File name: 8microtubuleslength.csv Content type: Data Description: Length of cellular microtubules after treatment with CA4 LONDs, measured from the images utilising ImageJ Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 11kB

Table 1 - LONDs formation summary [953kB] Table 1 - LONDs formation summary [953kB] Table 1 - LONDs formation summary [953kB] Visible to: Anyone File name: Table1.zip Content type: Data Description: Sizing and concentration measurements data used to producd the summary of LONDs formation for the different oil type. Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 953kB

Supplementary - CA4 in tripropionin calibration [51kB] Supplementary - CA4 in tripropionin calibration [51kB] Supplementary - CA4 in tripropionin calibration [51kB] Visible to: Anyone File name: supplementarycalibrationcurve.csv Content type: Data Description: Absorption of Combretastatin CA4 in triacetin, dissolved in DMSO, and used to produced a calibration curve to determine the concentration of CA4 encapsulated in LONDs samples Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 51kB

Supplementary - CA4 in tripropionin LONDs [15kB] Supplementary - CA4 in tripropionin LONDs [15kB] Supplementary - CA4 in tripropionin LONDs [15kB] Visible to: Anyone File name: supplementaryencapsulated.csv Content type: Data Description: Absorption of Combretastatin CA4 encapsulated in tripropionin LONDs samples. Maximum absorption is used to calculate the amount of drug encapsualted within the LONDs Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 15kB