Research Data Leeds Repository

Increased effect of microbubble-assisted gemcitabine delivery with repeated ultrasound exposure in a pancreatic cancer organ-on-a-chip model

Citation

Kpeglo, Delanyo and Haddrick, Malcolm and Knowles, Margaret A. and Evans, Stephen D. and Peyman, Sally A. (2025) Increased effect of microbubble-assisted gemcitabine delivery with repeated ultrasound exposure in a pancreatic cancer organ-on-a-chip model. University of Leeds. [Dataset] https://doi.org/10.5518/1218¹

Dataset description

The dataset are of raw images and collated data. The raw images are of subsequent assessment of the microfluidic PDAC cultures using a bright-field, epi-fluorescence, and confocal microscopy. The collated data include viability assessments of the microfluidic PDAC cultures following treatment with gemcitabine, microbubbles, and ultrasound exposure.

Keywords:	Organ-on-a-chip, microphysiological systems, tumour mechanics, tumour microenvironment, pancreatic ductal adenocarcinoma, microbubble and ultrasound-assisted therapeutic delivery
Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry2
Divisions:	Faculty of Engineering and Physical Sciences > School of Physics and Astronomy3
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	29 May 2025 07:43
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/14194
Additional details Additional details Alternative title: Increased effect of microbubble-assisted gemcitabine delivery with repeated ultrasound exposure in a pancreatic cancer organ-on-a-chip model Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Kpeglo, Delanyo https://orcid.org/0000-0001-8803-52645 University of Leeds https://ror.org/024mrxd33 ror Haddrick, Malcolm https://orcid.org/0000-0002-1146-69366 Medicines Discovery Catapult https://ror.org/00a3raj28 ror Knowles, Margaret A. https://orcid.org/0000-0002-9363-86577 University of Leeds https://ror.org/024mrxd33 ror Evans, Stephen D. https://orcid.org/0000-0001-8342-53358 University of Leeds https://ror.org/024mrxd33 ror Peyman, Sally A. https://orcid.org/0000-0002-1600-51009 Heriot-Watt University https://ror.org/04mghma93 ror Type of data: Dataset Research funders: Engineering and Physical Sciences Research Council Grant numbers: EP/P023266/1, EP/V026739/1 Publication date: 2025 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: D.Kpeglo@leeds.ac.uk10 Last Modified: 29 May 2025 07:57

Files

Documentation

README_Kpeglo-etal_2025.txt [3kB] 11 README_Kpeglo-etal_2025.txt [3kB] 12 README_Kpeglo-etal_2025.txt [3kB] 13 Security: Anyone File name: README_Kpeglo-etal_2025.txt14 Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 3kB

Data

Figure_1_and_Figure_S1.zip [388kB] 15 Figure_1_and_Figure_S1.zip [388kB] 16 Figure_1_and_Figure_S1.zip [388kB] 17 Security: Anyone File name: Figure_1_and_Figure_S1.zip18 Content type: Data Description: Schematic of microbubble and US treatment of the on-chip PDAC culture and CAD schematic of device used for the PDAC culture Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 388kB

Figure_2.zip [18MB] 19 Figure_2.zip [18MB] 20 Figure_2.zip [18MB] 21 Security: Anyone File name: Figure_2.zip22 Content type: Data Description: Bright-field images and ATP viability assessment of the microfluidic PDAC culture Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 18MB

Figure_3_and_Figure_S2_and_Supplementary_videos_1_and_2.zip [377MB] 23 Figure_3_and_Figure_S2_and_Supplementary_videos_1_and_2.zip [377MB] 24 Figure_3_and_Figure_S2_and_Supplementary_videos_1_and_2.zip [377MB] 25 Security: Anyone File name: Figure_3_and_Figure_S2_and_Supplementary_videos_1_and_2.zip26 Content type: Data Description: Images of the ATTO488 labelled microbubbles on-chip with the microfluidic PDAC culture or BME gel only Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 377MB

Figure_4_and_Figure 5_and_Figure_6_and_Figure_7.zip [326MB] 27 Figure_4_and_Figure 5_and_Figure_6_and_Figure_7.zip [326MB] 28 Figure_4_and_Figure 5_and_Figure_6_and_Figure_7.zip [326MB] 29 Security: Anyone File name: Figure_4_and_Figure_5_and_Figure_6_and_Figure_7.zip30 Content type: Data Description: Timeline of the seeding of PDAC cells for culture and treatment, ATP viability assessment of 2D cultured and microfluidic cultured PDAC cells treated with gemcitabine, microbubbles, and ultrasound exposure, and quantification and images of the microfluidi Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 326MB

• 1. https://doi.org/10.5518/1218
• 2. https://archive.researchdata.leeds.ac.uk/view/subjects/C700.html
• 3. https://archive.researchdata.leeds.ac.uk/view/divisions/SPA/
• 4. https://archive.researchdata.leeds.ac.uk/id/eprint/1419
• 5. https://orcid.org/0000-0001-8803-5264
• 6. https://orcid.org/0000-0002-1146-6936
• 7. https://orcid.org/0000-0002-9363-8657
• 8. https://orcid.org/0000-0001-8342-5335
• 9. https://orcid.org/0000-0002-1600-5100
• 10. mailto:D.Kpeglo@leeds.ac.uk
• 11. https://archive.researchdata.leeds.ac.uk/1419/5/README_Kpeglo-etal_2025.txt
• 12. https://archive.researchdata.leeds.ac.uk/1419/5/README_Kpeglo-etal_2025.txt
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• 14. https://archive.researchdata.leeds.ac.uk/1419/5/README_Kpeglo-etal_2025.txt
• 15. https://archive.researchdata.leeds.ac.uk/1419/1/Figure_1_and_Figure_S1.zip
• 16. https://archive.researchdata.leeds.ac.uk/1419/1/Figure_1_and_Figure_S1.zip
• 17. https://archive.researchdata.leeds.ac.uk/1419/1/Figure_1_and_Figure_S1.zip
• 18. https://archive.researchdata.leeds.ac.uk/1419/1/Figure_1_and_Figure_S1.zip
• 19. https://archive.researchdata.leeds.ac.uk/1419/2/Figure_2.zip
• 20. https://archive.researchdata.leeds.ac.uk/1419/2/Figure_2.zip
• 21. https://archive.researchdata.leeds.ac.uk/1419/2/Figure_2.zip
• 22. https://archive.researchdata.leeds.ac.uk/1419/2/Figure_2.zip
• 23. https://archive.researchdata.leeds.ac.uk/1419/3/Figure_3_and_Figure_S2_and_Supplementary_videos_1_and_2.zip
• 24. https://archive.researchdata.leeds.ac.uk/1419/3/Figure_3_and_Figure_S2_and_Supplementary_videos_1_and_2.zip
• 25. https://archive.researchdata.leeds.ac.uk/1419/3/Figure_3_and_Figure_S2_and_Supplementary_videos_1_and_2.zip
• 26. https://archive.researchdata.leeds.ac.uk/1419/3/Figure_3_and_Figure_S2_and_Supplementary_videos_1_and_2.zip
• 27. https://archive.researchdata.leeds.ac.uk/1419/4/Figure_4_and_Figure_5_and_Figure_6_and_Figure_7.zip
• 28. https://archive.researchdata.leeds.ac.uk/1419/4/Figure_4_and_Figure_5_and_Figure_6_and_Figure_7.zip
• 29. https://archive.researchdata.leeds.ac.uk/1419/4/Figure_4_and_Figure_5_and_Figure_6_and_Figure_7.zip
• 30. https://archive.researchdata.leeds.ac.uk/1419/4/Figure_4_and_Figure_5_and_Figure_6_and_Figure_7.zip