Research Data Leeds Repository

Citation

Taylor, Alexander I. P., Xu, Yong, Wilkinson, Martin, Chakraborty, Pijush, Brinkworth, Alice, Willis, Leon, Zhuravleva, Anastasia, Ranson, Neil A., Foster, Richard and Radford, Sheena E. (2025) Data for 'Kinetic Steering of Amyloid Formation'. University of Leeds. [Dataset] https://doi.org/10.5518/1604

Dataset description

Data for the figures in "Kinetic Steering of Amyloid Formation by Canagliflozin, a Type-2 Diabetes Drug" by Taylor et al. (JACS, 2025).

Subjects:	C000 - Biological sciences F000 - Physical sciences > F100 - Chemistry
Divisions:	Faculty of Biological Sciences > Astbury Centre for Structural Molecular Biology Faculty of Biological Sciences > School of Molecular and Cellular Biology Faculty of Engineering and Physical Sciences > School of Chemistry
Related resources:	Location Type https://doi.org/10.1021/jacs.4c16743 Publication https://eprints.whiterose.ac.uk/223329/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	03 Mar 2025 16:26
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/1380
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Taylor, Alexander I. P. https://orcid.org/0000-0003-3131-3619 University of Leeds https://ror.org/024mrxd33 ror Xu, Yong https://orcid.org/0000-0002-8389-4120 University of Leeds https://ror.org/024mrxd33 ror Wilkinson, Martin https://orcid.org/0000-0001-5490-613X University of Leeds https://ror.org/024mrxd33 ror Chakraborty, Pijush https://orcid.org/0000-0003-1749-5225 University of Leeds https://ror.org/024mrxd33 ror Brinkworth, Alice https://orcid.org/0009-0007-3385-5784 University of Leeds https://ror.org/024mrxd33 ror Willis, Leon https://orcid.org/0000-0001-6616-3716 University of Leeds https://ror.org/024mrxd33 ror Zhuravleva, Anastasia https://orcid.org/0000-0001-6165-8509 University of Leeds https://ror.org/024mrxd33 ror Ranson, Neil A. https://orcid.org/0000-0002-3640-5275 University of Leeds https://ror.org/024mrxd33 ror Foster, Richard https://orcid.org/0000-0002-2361-3884 University of Leeds https://ror.org/024mrxd33 ror Radford, Sheena E. https://orcid.org/0000-0002-3079-8039 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: Wellcome, MRC, EPSRC, Royal Society Grant numbers: Wellcome 204963, MRC MR/T011149/1, EPSRC APP12867, Royal Society RSRP\R1\211057 Publication date: 2025 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: a.taylor10@leeds.ac.uk, s.e.radford@leeds.ac.uk Last Modified: 06 Mar 2025 14:38

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Documentation

README_Taylor_2025.txt [3kB] README_Taylor_2025.txt [3kB] README_Taylor_2025.txt [3kB] Security: Anyone File name: README_Taylor_2025.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 3kB

Data

ThT kinetics for the preliminary SAR (Fig. S1) [30kB] ThT kinetics for the preliminary SAR (Fig. S1) [30kB] ThT kinetics for the preliminary SAR (Fig. S1) [30kB] Security: Anyone File name: 1_ThT_preliminary_SAR.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 30kB

ROCS searches against YX-I-1 enantiomer 1 [538kB] ROCS searches against YX-I-1 enantiomer 1 [538kB] ROCS searches against YX-I-1 enantiomer 1 [538kB] Security: Anyone File name: 2_ROCS_enantiomer_1.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 538kB

ROCS searches against YX-I-1 enantiomer 2 [534kB] ROCS searches against YX-I-1 enantiomer 2 [534kB] ROCS searches against YX-I-1 enantiomer 2 [534kB] Security: Anyone File name: 3_ROCS_enantiomer_2.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 534kB

Compound solubility screening by absorbance spectroscopy (Fig. S3) [149kB] Compound solubility screening by absorbance spectroscopy (Fig. S3) [149kB] Compound solubility screening by absorbance spectroscopy (Fig. S3) [149kB] Security: Anyone File name: 4_absorbance_compound_solubility.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 149kB

Compound solubility screening by Capflex (Fig. S3) [565kB] Compound solubility screening by Capflex (Fig. S3) [565kB] Compound solubility screening by Capflex (Fig. S3) [565kB] Security: Anyone File name: 5_Capflex_compound_solubility.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 565kB

Additional TDA experiments to confirm compound solubility (Fig. S4) [1MB] Additional TDA experiments to confirm compound solubility (Fig. S4) [1MB] Additional TDA experiments to confirm compound solubility (Fig. S4) [1MB] Security: Anyone File name: 6_TDA_compound_solubility.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

ThT kinetics to investigate the assembly mechanism of IAPP (Fig. S5) [92kB] ThT kinetics to investigate the assembly mechanism of IAPP (Fig. S5) [92kB] ThT kinetics to investigate the assembly mechanism of IAPP (Fig. S5) [92kB] Security: Anyone File name: 7_ThT_assembly_mechanism.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 92kB

ThT kinetics to screen for inhibitors of IAPP assembly (Fig. 2, Fig. S6) [99kB] ThT kinetics to screen for inhibitors of IAPP assembly (Fig. 2, Fig. S6) [99kB] ThT kinetics to screen for inhibitors of IAPP assembly (Fig. 2, Fig. S6) [99kB] Security: Anyone File name: 8_ThT_kinetic_screening.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 99kB

ThT kinetics to test the sequence-specificity of compounds (Fig. S7) [8kB] ThT kinetics to test the sequence-specificity of compounds (Fig. S7) [8kB] ThT kinetics to test the sequence-specificity of compounds (Fig. S7) [8kB] Security: Anyone File name: 9_ThT_sequence_specificity.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8kB

ThT kinetics of IAPP at varying compound concentrations (Fig. 3) [115kB] ThT kinetics of IAPP at varying compound concentrations (Fig. 3) [115kB] ThT kinetics of IAPP at varying compound concentrations (Fig. 3) [115kB] Security: Anyone File name: 10_ThT_compound_dependence.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 115kB

ThT kinetics for pelleting of IAPP at intermediate assembly stages (Fig. S9) [20kB] ThT kinetics for pelleting of IAPP at intermediate assembly stages (Fig. S9) [20kB] ThT kinetics for pelleting of IAPP at intermediate assembly stages (Fig. S9) [20kB] Security: Anyone File name: 11_ThT_intermediate_pelleting.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 20kB

HPLC analysis for pelleting of IAPP at intermediate assembly stages (Fig. S9) [527kB] HPLC analysis for pelleting of IAPP at intermediate assembly stages (Fig. S9) [527kB] HPLC analysis for pelleting of IAPP at intermediate assembly stages (Fig. S9) [527kB] Security: Anyone File name: 12_HPLC_intermediate_pelleting.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 527kB

SPR experiments to evaluate binding of compounds to IAPP monomer (Fig. 4) [228kB] SPR experiments to evaluate binding of compounds to IAPP monomer (Fig. 4) [228kB] SPR experiments to evaluate binding of compounds to IAPP monomer (Fig. 4) [228kB] Security: Anyone File name: 13_SPR_monomer_binding.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 228kB

SPR titration of canagliflozin against IAPP monomer (Fig. S11) [313kB] SPR titration of canagliflozin against IAPP monomer (Fig. S11) [313kB] SPR titration of canagliflozin against IAPP monomer (Fig. S11) [313kB] Security: Anyone File name: 14_SPR_canagliflozin_titration.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 313kB

NMR data for the paper (Fig. 4, Fig. 5, Fig. S12) [10MB] NMR data for the paper (Fig. 4, Fig. 5, Fig. S12) [10MB] NMR data for the paper (Fig. 4, Fig. 5, Fig. S12) [10MB] Security: Anyone File name: 15_NMR_data.zip Content type: Data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 10MB

Absorbance spectra for co-pelleting of fibrils and compounds (Fig. 6) [51kB] Absorbance spectra for co-pelleting of fibrils and compounds (Fig. 6) [51kB] Absorbance spectra for co-pelleting of fibrils and compounds (Fig. 6) [51kB] Security: Anyone File name: 16_absorbance_compound_pelleting.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 51kB

Capflex data showing binding of compounds to fibrils (Fig. 6) [140kB] Capflex data showing binding of compounds to fibrils (Fig. 6) [140kB] Capflex data showing binding of compounds to fibrils (Fig. 6) [140kB] Security: Anyone File name: 17_Capflex_compound_pelleting.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 140kB

Fibril crossover distributions from cryo-EM (Fig. 7) [909B] Fibril crossover distributions from cryo-EM (Fig. 7) [909B] Fibril crossover distributions from cryo-EM (Fig. 7) [909B] Security: Anyone File name: 18_EM_fibril_crossovers.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 909B

Fibril subunit fold distributions from cryo-EM (Fig. 8) [167B] Fibril subunit fold distributions from cryo-EM (Fig. 8) [167B] Fibril subunit fold distributions from cryo-EM (Fig. 8) [167B] Security: Anyone File name: 19_EM_fibril_subunit_folds.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 167B

ThT kinetics of seeded IAPP assembly (Fig. S17) [21kB] ThT kinetics of seeded IAPP assembly (Fig. S17) [21kB] ThT kinetics of seeded IAPP assembly (Fig. S17) [21kB] Security: Anyone File name: 20_ThT_seeded_aggregation.csv Content type: Data Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 21kB