Research Data Leeds Repository

Ire1-LD activation by unfolded protein substrate and molecular chaperone BiP

Citation

Zhuravleva, Anastasia and Hurst, Nicholas and Dawes, Sam and Grey, Gabriel (2024) Ire1-LD activation by unfolded protein substrate and molecular chaperone BiP. University of Leeds. [Dataset] https://doi.org/10.5518/1533¹

Dataset description

Biophysical characterization of how the molecular chaperone BiP and a model Ire1 substrate interdependently control activation of the ER stress sensor Ire1

Keywords:	Ire1, Hsp70, UPR
Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry2
Divisions:	Faculty of Biological Sciences > School of Molecular and Cellular Biology3
Related resources:	Location Type https://doi.org/10.1101/2024.02.16.5796204 Publication https://doi.org/10.26508/lsa.2024027025 Publication https://eprints.whiterose.ac.uk/216208/6 Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	24 Jul 2024 12:36
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/13077
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Zhuravleva, Anastasia https://orcid.org/0000-0001-6165-85098 University of Leeds https://ror.org/024mrxd33 ror Hurst, Nicholas https://orcid.org/0000-0003-3850-66409 University of Leeds https://ror.org/024mrxd33 ror Dawes, Sam University of Leeds https://ror.org/024mrxd33 ror Grey, Gabriel University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: BBSRC Grant numbers: BB/M021874/1 Publication date: 2024 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: fbsazh@leeds.ac.uk10 Last Modified: 24 Jan 2025 11:11

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Documentation

readme [10kB] 11 readme [10kB] 12 readme [10kB] 13 Security: Anyone File name: readme-DOI_1533.txt14 Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 10kB

Data

Construct details [19kB] 15 Construct details [19kB] 16 Construct details [19kB] 17 Security: Anyone File name: Constructinfo.xlsx18 Content type: Data Description: A.a. sequences of all protein constructs used in the study Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 19kB

Data for Supplementary Fig 12 [1MB] 19 Data for Supplementary Fig 12 [1MB] 20 Data for Supplementary Fig 12 [1MB] 21 Security: Anyone File name: SupplementaryFig12.zip22 Content type: Data Description: Full images of SDS-PAGE gels for Supplementary Fig 12 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

Data for Supplementary Fig 14 [762kB] 23 Data for Supplementary Fig 14 [762kB] 24 Data for Supplementary Fig 14 [762kB] 25 Security: Anyone File name: SupplementaryFig14.zip26 Content type: Data Description: Full images of SDS-PAGE gels for Supplementary Fig 14 Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 762kB

MST data (Figure 1a) [18kB] 27 MST data (Figure 1a) [18kB] 28 MST data (Figure 1a) [18kB] 29 Security: Anyone File name: Figure1aMST.xlsx30 Content type: Data Description: Raw data for Figure 1a Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 18kB

DLS data (Figure 1b and 2a) [525kB] 31 DLS data (Figure 1b and 2a) [525kB] 32 DLS data (Figure 1b and 2a) [525kB] 33 Security: Anyone File name: Figure1band2aDLSraw.zip34 Content type: Data Description: Raw data for Figures 1b and 2a Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 525kB

Data for Figure 2b [22kB] 35 Data for Figure 2b [22kB] 36 Data for Figure 2b [22kB] 37 Security: Anyone File name: Figure2b.xlsx38 Content type: Data Description: Solubility assay data for Figure 2b Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 22kB

Data for Figure 2c [18MB] 39 Data for Figure 2c [18MB] 40 Data for Figure 2c [18MB] 41 Security: Anyone File name: Figure2c.png42 Content type: Data Description: Full image of SDS-PAGE gel for Figure 2c Mime-Type: image/png License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 18MB

Data for Figure 2d [14MB] 43 Data for Figure 2d [14MB] 44 Data for Figure 2d [14MB] 45 Security: Anyone File name: Figure2d.png46 Content type: Data Description: Full image of SDS-PAGE gel for Figure 2d Mime-Type: image/png License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 14MB

Data for Figure 3b [34kB] 47 Data for Figure 3b [34kB] 48 Data for Figure 3b [34kB] 49 Security: Anyone File name: Figure3bATP.xlsx50 Content type: Data Description: ATPase measurements for Figure 3b Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 34kB

Data for Figure 4c [30kB] 51 Data for Figure 4c [30kB] 52 Data for Figure 4c [30kB] 53 Security: Anyone File name: Figure4c.xlsx54 Content type: Data Description: Conservation scores for Figure 4c Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 30kB

Data for Supplementary Fig 11b [28kB] 55 Data for Supplementary Fig 11b [28kB] 56 Data for Supplementary Fig 11b [28kB] 57 Security: Anyone File name: SupplementaryFig11b.xlsx58 Content type: Data Description: NMR peak intensities for Supplementary Fig 11b Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 28kB

Consurf Outputs for Supplementary Figure 13 [1MB] 59 Consurf Outputs for Supplementary Figure 13 [1MB] 60 Consurf Outputs for Supplementary Figure 13 [1MB] 61 Security: Anyone File name: SupplementaryFig13ConsurfOutputs.zip62 Content type: Data Description: Consurf Outputs Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

Data for Supplementary Fig 15b [27kB] 63 Data for Supplementary Fig 15b [27kB] 64 Data for Supplementary Fig 15b [27kB] 65 Security: Anyone File name: SupplementaryFig15b.xlsx66 Content type: Data Description: NMR peak intensities for Supplementary Fig 15b Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 27kB

Data for Supplementary Fig 15c [29kB] 67 Data for Supplementary Fig 15c [29kB] 68 Data for Supplementary Fig 15c [29kB] 69 Security: Anyone File name: SupplementaryFig15c.xlsx70 Content type: Data Description: NMR data used to calculate temperature coefficients shown in Figure 15c Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 29kB

Data for Supplementary Figure 16 [44kB] 71 Data for Supplementary Figure 16 [44kB] 72 Data for Supplementary Figure 16 [44kB] 73 Security: Anyone File name: SupplementaryFig16.xlsx74 Content type: Data Description: IUPred3, ESpritz, MoreRONN and PONDR predictions Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 44kB

NMR data for Supplementary Figure 8a [3MB] 75 NMR data for Supplementary Figure 8a [3MB] 76 NMR data for Supplementary Figure 8a [3MB] 77 Security: Anyone File name: SupplementaryFig8assNMRraw.zip78 Content type: Data Description: Bruker NMR spectrum, raw and NMRPile formats Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 3MB

Data for Supplementary Fig 9 [437kB] 79 Data for Supplementary Fig 9 [437kB] 80 Data for Supplementary Fig 9 [437kB] 81 Security: Anyone File name: SupplementaryFig9.xlsx82 Content type: Data Description: FIDA data for Supplementary Figure 9 Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 437kB

Data for Supplementary Figure 16 [87kB] 83 Data for Supplementary Figure 16 [87kB] 84 Data for Supplementary Figure 16 [87kB] 85 Security: Anyone File name: SupplementaryFig16DISOPRED3.png86 Content type: Data Description: DISOPRED3 prediction Mime-Type: image/png License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 87kB

Data for Supplementary Figure 17b [21kB] 87 Data for Supplementary Figure 17b [21kB] 88 Data for Supplementary Figure 17b [21kB] 89 Security: Anyone File name: SupplementaryFig17.xlsx90 Content type: Data Description: Bradford assay results for Supplementary Figure 17b Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 21kB

Data for Supplementary Table 1 [6kB] 91 Data for Supplementary Table 1 [6kB] 92 Data for Supplementary Table 1 [6kB] 93 Security: Anyone File name: SupplementaryTable1.csv94 Content type: Data Description: BipPred results Mime-Type: text/csv License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 6kB

NMR data for Supplementary Figure 11 [63MB] 95 NMR data for Supplementary Figure 11 [63MB] 96 NMR data for Supplementary Figure 11 [63MB] 97 Security: Anyone File name: SupplementaryFig11NMR.zip98 Content type: Data Description: Bruker NMR spectrum, raw and NMRPile formats Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 63MB

Data for Supplementary Figure 4 [45kB] 99 Data for Supplementary Figure 4 [45kB] 100 Data for Supplementary Figure 4 [45kB] 101 Security: Anyone File name: SupplementaryFig4.xlsx102 Content type: Data Description: Turbidity meadurements for Supplementary Figure 4 Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 45kB

Data for Supplementary Figures 6 and 7 [667kB] 103 Data for Supplementary Figures 6 and 7 [667kB] 104 Data for Supplementary Figures 6 and 7 [667kB] 105 Security: Anyone File name: SupplementaryFig6-7.xlsx106 Content type: Data Description: Turbidity meadurements for Supplementary Figures 6 and 7 Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 667kB

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