Research Data Leeds Repository

Modelling and breaking down the biophysical barriers to drug delivery in pancreatic cancer

Kpeglo, Delanyo (2024) Modelling and breaking down the biophysical barriers to drug delivery in pancreatic cancer. University of Leeds. [Dataset] https://doi.org/10.5518/1297

Dataset description

The dataset are of raw images and collated data. The raw images are of subsequent assessment of the microfluidic PDAC cultures using a bright-field, epi-fluorescence, and confocal microscopy. The collated data are of the viability assessments, immunofluorescence assessmetns, of the microfluidic PDAC cultures following treatment with gemcitabine and losartan, and of the interstitial flow assessments.

Keywords:	Organ-on-chip; tumour mechanics; tumour microenvironment; pancreatic ductal adenocarcinoma; matrix modifier
Divisions:	Faculty of Engineering and Physical Sciences > School of Physics and Astronomy
Related resources:	Location Type https://doi.org/10.1039/D3LC00660C Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	31 Jan 2024 21:06
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/1233
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Kpeglo, Delanyo https://orcid.org/0000-0001-8803-5264 University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: Engineering and Physical Sciences Research Council, Engineering and Physical Sciences Research Council, Royal Society of Chemistry Grant numbers: EP/P023266/1, EP/V026739/1, E21-6195497927 Publication date: 2024 Resource language: English Publisher: University of Leeds Contact Email Address: phydk@leeds.ac.uk Last Modified: 11 Mar 2024 10:48

Files

Documentation

file_manifest.txt [84kB] file_manifest.txt [84kB] file_manifest.txt [84kB] Visible to: Anyone File name: file_manifest.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 84kB

Data

Figure_1.zip [6MB] Figure_1.zip [6MB] Figure_1.zip [6MB] Visible to: Anyone File name: Figure_1.zip Content type: Data Description: CAD schematic of device, time line of seeding cells into the device for culture and assessments, and image of culture model 24 hours after seeding Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 6MB

Figure_2_Figure_3_Figure_5_and_S2_and_S3.zip [958MB] Figure_2_Figure_3_Figure_5_and_S2_and_S3.zip [958MB] Figure_2_Figure_3_Figure_5_and_S2_and_S3.zip [958MB] Visible to: Anyone File name: Figure_2_Figure_3_Figure_5_and_S2_and_S3.zip Content type: Data Description: ATP viability assessment of the developing microfluidic PDAC culture, confocal images of the live/dead viability assessment of the PDAC cultrue, and of the immunofluorescence assessment of the microfluidic PDAC culture for collagen and HIF-1 alpha Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 958MB

Figure_4_Figure_8B_Table_1_Supplementary_Figure_4_and_Supplementary_videos_1_and_2.zip [67MB] Figure_4_Figure_8B_Table_1_Supplementary_Figure_4_and_Supplementary_videos_1_and_2.zip [67MB] Figure_4_Figure_8B_Table_1_Supplementary_Figure_4_and_Supplementary_videos_1_and_2.zip [67MB] Visible to: Anyone File name: Figure_4_Figure_8B_Table_1_Supplementary_Figure_4_and_Supplementary_videos_1_and_2.zip Content type: Data Description: Images, videos, and data of the interstitial flow assessment of the microfluidic PDAC culture without and with losartan treatment - the hydraulic conductivity of the culture interstitial space and the intensity profile of the perfusion of fluorescein-medi Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 67MB

Figure_6_Figure_7_Figure_8A_and_S1_S5_and_S6.zip [3GB] Figure_6_Figure_7_Figure_8A_and_S1_S5_and_S6.zip [3GB] Figure_6_Figure_7_Figure_8A_and_S1_S5_and_S6.zip [3GB] Visible to: Anyone File name: Figure_6_Figure_7_Figure_8A_and_S1_S5_and_S6.zip Content type: Data Description: ATP viability assessment of the microfluidic PDAC culture treated with gemcitabine and losartan, confocal images of the immunostained microfluidic and off-chip PDAC cultures for collagen and HIF-1 alpha, and the moduli of the cultures following treatment Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 3GB