Research Data Leeds Repository

Dynamic coupling of fast channel gating with slow ATP-turnover underpins protein transport through the Sec-translocon

Crossley, Joel A. and Allen, William J. and Watkins, Daniel W. and Sabir, Tara and Radford, Sheena E. and Tuma, Roman and Collinson, Ian and Fessl, Tomas (2023) Dynamic coupling of fast channel gating with slow ATP-turnover underpins protein transport through the Sec-translocon. University of Leeds. [Dataset] https://doi.org/10.5518/1410

Dataset description

The Sec-translocon is a highly conserved membrane complex for polypeptide transport across, or into, lipid bilayers. In bacteria, the core protein-channel complex SecYEG resides in the inner-membrane, through which secretion is powered by the cytosolic ATPase SecA. Here, we present a single-molecule FRET dataset which shows that the SecYEG-channel fluctuates between open and closed states much faster than ATP turnover, while the nucleotide status of SecA modulates the rates of opening and closure.

Keywords:	Protein dynamics, Protein translocation, SecYEG, SecA, Single‐molecule FRET
Subjects:	C000 - Biological sciences
Divisions:	Faculty of Biological Sciences > Astbury Centre for Structural Molecular Biology Faculty of Biological Sciences > School of Molecular and Cellular Biology
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	27 Nov 2023 21:06
URI:	http://archive.researchdata.leeds.ac.uk/id/eprint/1197
Additional details Additional details Creators: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Crossley, Joel A. https://orcid.org/0000-0002-6656-1578 University of Leeds Allen, William J. https://orcid.org/0000-0002-9513-4786 University of Bristol Watkins, Daniel W. https://orcid.org/0000-0003-3825-5036 University of Bristol Sabir, Tara https://orcid.org/0000-0002-3268-1344 Leeds Beckett University Radford, Sheena E. https://orcid.org/0000-0002-3079-8039 University of Leeds Tuma, Roman https://orcid.org/0000-0003-0047-0013 University of South Bohemia Collinson, Ian https://orcid.org/0000-0002-3931-0503 University of Bristol Fessl, Tomas https://orcid.org/0000-0001-6969-4870 University of South Bohemia Type of data: Dataset Research funders: BBSRC, Royal Society Professorial Research Fellowship, European Regional Development Fund, Czech Science Foundation Grant numbers: BB/T008059/1, BB/V001531/1, BB/T006889/1, RSRP\R1\211057, CZ.02.1.01/0.0/0.0/15_003/0000441 Publication date: 2023 Resource language: English Publisher: University of Leeds Contact Email Address: j.a.crossley@leeds.ac.uk Last Modified: 28 Nov 2023 12:16

Files

Data

smFRET data [10GB] smFRET data [10GB] smFRET data [10GB] Visible to: Anyone File name: smFRET_data.zip Content type: Data Description: A .zip file containing .hdf5 files containing photon arrival times for single-molecule FRET experiments. Each experimental condition is comprised of five technical repeats. Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 10GB

README_Crossley_2023.txt [3kB] README_Crossley_2023.txt [3kB] README_Crossley_2023.txt [3kB] Visible to: Anyone File name: README_Crossley_2023.txt Content type: Data Mime-Type: text/plain File size: 3kB