1. ABOUT THE DATASET -------------------- Title: Energy landscape steering mediates dynamic coupling in ATP-driven protein translocation. [Dataset]. https://doi.org/10.5518/1410 Creator(s): Joel A. Crossley[1,2,3], William J. Allen[4], Daniel W. Watkins[4], Tara Sabir[3], Sheena E. Radford[1], Roman Tuma[1,2], Ian Collinson[4] and Tomas Fessl[2] Organisation(s): [1] Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, United Kingdom [2] Faculty of Science, University of South Bohemia, České Budějovice, 370 05, Czech Republic [3] School of Clinical and Applied Sciences, Leeds Beckett University, Leeds, LS1 3HE, United Kingdom [4] School of Biochemistry, University of Bristol, Bristol, BS8 1QU, United Kingdom Rights-holder(s): Unless otherwise stated, Copyright 2023 University of Leeds Publication Year: 2023 Description: The Sec-translocon is a highly conserved membrane complex for polypeptide transport across, or into, lipid bilayers. In bacteria, the core protein-channel complex SecYEG resides in the inner-membrane, through which secretion is powered by the cytosolic ATPase SecA. Here, we present a single-molecule FRET dataset which shows that the SecYEG-channel fluctuates between open and closed states much faster than ATP turnover, while the nucleotide status of SecA modulates the rates of opening and closure. Cite as: Joel A. Crossley, William J. Allen, Daniel W. Watkins, Tara Sabir, Sheena E. Radford, Roman Tuma, Ian Collinson and Tomas Fessl (2023): Energy landscape steering mediates dynamic coupling in ATP-driven protein translocation. University of Leeds. [Dataset] https://doi.org/10.5518/1410. Related publication: Title: 'Energy landscape steering mediates dynamic coupling in ATP-driven protein translocation'. Year: 2023. Authors: Joel A. Crossley, William J. Allen, Daniel W. Watkins, Tara Sabir, Sheena E. Radford, Roman Tuma, Ian Collinson and Tomas Fessl. (EMBO, accepted). Contact: j.a.crossley@leeds.ac.uk, fessl@prf.jcu.cz and ian.collinson@bristol.ac.uk 2. TERMS OF USE --------------- Copyright 2023 University of Leeds. Unless otherwise stated, this dataset is licensed under a Creative Commons Attribution 4.0 International Licence: https://creativecommons.org/licenses/by/4.0/. 3. PROJECT AND FUNDING INFORMATION ---------------------------------- This work was funded by the BBSRC: J.A.C., R.T. and S.E.R. (BB/T008059/1); W.J.A. and I.C. (BB/V001531/1); D.W.W and I.C. (BB/T006889/1). SER holds a Royal Society Professorial Research Fellowship (RSRP\R1\211057). J.A.C., T.F. and R.T. are supported by the European Regional Development Fund-Project (CZ.02.1.01/0.0/0.0/15_003/0000441) and the Czech Science Foundation (20-11563Y). J.A.C. and T.S. also acknowledge Leeds Beckett University for funding. 4. CONTENTS ----------- File listing smFRET_data.zip - A .zip file containing .hdf5 files containing photon arrival times for single-molecule FRET experiments. Each experimental condition is comprised of five technical repeats. 5. METHODS ---------- Single-molecule Förster resonance energy transfer (smFRET) experiments were performed on a custom-built confocal epi-illuminated microscope in a standard inverted-stage configuration with a pulsed interleaved excitation regime.