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Dynamic coupling of fast channel gating with slow ATP-turnover underpins protein transport through the Sec-translocon

Citation

Crossley, Joel A. and Allen, William J. and Watkins, Daniel W. and Sabir, Tara and Radford, Sheena E. and Tuma, Roman and Collinson, Ian and Fessl, Tomas (2023) Dynamic coupling of fast channel gating with slow ATP-turnover underpins protein transport through the Sec-translocon. University of Leeds. [Dataset] https://doi.org/10.5518/1410

Dataset description

The Sec-translocon is a highly conserved membrane complex for polypeptide transport across, or into, lipid bilayers. In bacteria, the core protein-channel complex SecYEG resides in the inner-membrane, through which secretion is powered by the cytosolic ATPase SecA. Here, we present a single-molecule FRET dataset which shows that the SecYEG-channel fluctuates between open and closed states much faster than ATP turnover, while the nucleotide status of SecA modulates the rates of opening and closure.

Keywords: Protein dynamics, Protein translocation, SecYEG, SecA, Single‐molecule FRET
Subjects: C000 - Biological sciences
Divisions: Faculty of Biological Sciences > Astbury Centre for Structural Molecular Biology
Faculty of Biological Sciences > School of Molecular and Cellular Biology
Related resources:
LocationType
https://doi.org/10.1038/s44318-023-00004-1Publication
https://eprints.whiterose.ac.uk/207414/Publication
License: Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited: 27 Nov 2023 21:06
URI: https://archive.researchdata.leeds.ac.uk/id/eprint/1197

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