Research Data Leeds Repository

Citation

Machin, Jonathan (2023) Protein–lipid charge interactions control the folding of outer membrane proteins into asymmetric membranes. University of Leeds. [Dataset] https://doi.org/10.5518/1168

Dataset description

Raw data files

Subjects:	C000 - Biological sciences > C700 - Molecular biology, biophysics & biochemistry
Divisions:	Faculty of Biological Sciences > Astbury Centre for Structural Molecular Biology
Related resources:	Location Type https://doi.org/10.1038/s41557-023-01319-6 Publication https://eprints.whiterose.ac.uk/203469/ Publication
License:	Creative Commons Attribution 4.0 International (CC BY 4.0)
Date deposited:	05 Sep 2023 20:06
URI:	https://archive.researchdata.leeds.ac.uk/id/eprint/1156
Additional details Additional details Creators or authors: Creators ORCID Other ID Email Primary affiliation Primary affiliation ID Primary affiliation ID type Secondary affiliation Secondary affiliation ID Secondary affiliation ID type Machin, Jonathan https://orcid.org/0000-0002-1920-149X University of Leeds https://ror.org/024mrxd33 ror Type of data: Dataset Research funders: Wellcome Trust, Royal Society Grant numbers: J.M.M is funded by Wellcome (222373/Z/21/Z). CryoEM imaging was carried out at the Astbury Biostructure Laboratory, which was funded by the University of Leeds and Wellcome (108466/Z/15/Z & 221524/Z/20/Z), SER holds a Royal Society Professorial Research Felowship Publication date: 2023 Resource language: English Metadata language: English Publisher: University of Leeds Contact email: bsjma@leeds.ac.uk Last Modified: 20 May 2024 10:54

Files

Documentation

README.txt [1kB] README.txt [1kB] README.txt [1kB] Security: Anyone File name: README.txt Content type: Documentation Mime-Type: text/plain License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1kB

Data

0figure_data [2MB] 0figure_data [2MB] 0figure_data [2MB] Security: Anyone File name: 0figure_data.zip Content type: Data Description: excel files containing the underlying data used to generate each figure Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 2MB

absorbance [106kB] absorbance [106kB] absorbance [106kB] Security: Anyone File name: absorbance.zip Content type: Data Description: all raw absorbance data (Stewart assay and exchange of DPPE-Rho doped liposomes) Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 106kB

bioinformatics [6MB] bioinformatics [6MB] bioinformatics [6MB] Security: Anyone File name: bioinformatics.zip Content type: Data Description: tables containing accession codes for all bioinformatic datasets Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 6MB

dmpe-pc_FRET_asymmetry [33kB] dmpe-pc_FRET_asymmetry [33kB] dmpe-pc_FRET_asymmetry [33kB] Security: Anyone File name: dmpe-pc_FRET_asymmetry.zip Content type: Data Description: raw data for controls and samples demonstrating the asymmetry of DMPE/DMPC liposomes Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 33kB

folding kinetics [609kB] folding kinetics [609kB] folding kinetics [609kB] Security: Anyone File name: folding_kinetics.zip Content type: Data Description: all raw folding kinetic data and fitted parameters Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 609kB

gels [161MB] gels [161MB] gels [161MB] Security: Anyone File name: gels.zip Content type: Data Description: raw images of all gels and resultant densitometry analysis Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 161MB

laurdan [88kB] laurdan [88kB] laurdan [88kB] Security: Anyone File name: laurdan.zip Content type: Data Description: raw and processed fluorescent data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 88kB

liposome_zeta_DLS [101kB] liposome_zeta_DLS [101kB] liposome_zeta_DLS [101kB] Security: Anyone File name: liposome_zeta_DLS.zip Content type: Data Description: all DLS and zeta data for liposome validation Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 101kB

Molecular dynamics [8GB] Molecular dynamics [8GB] Molecular dynamics [8GB] Security: Anyone File name: Molecular_dynamics.zip Content type: Data Description: mdp and tpr files for all simualations, and final gro files with thinned trajectories (every 20 frames) Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 8GB

supplementery_tables [1MB] supplementery_tables [1MB] supplementery_tables [1MB] Security: Anyone File name: supplementry_tables.zip Content type: Data Description: the three external (excel) supplementary table files Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 1MB

thermodynamics_fluorescence.zip [163kB] thermodynamics_fluorescence.zip [163kB] thermodynamics_fluorescence.zip [163kB] Security: Anyone File name: thermodynamics_fluorescence.zip Content type: Data Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 163kB

zeta_prediction [13kB] zeta_prediction [13kB] zeta_prediction [13kB] Security: Anyone File name: zeta_prediction.zip Content type: Data Description: raw zeta potential data collated for model, and the model code Mime-Type: application/zip License: Creative Commons Attribution 4.0 International (CC BY 4.0) File size: 13kB